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EMAGE:18990

Tprn taperin ( MGI:2139535)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18990 EMAGE:18990 EMAGE:18990 EMAGE:18990 EMAGE:18990
"Pseudo-wholemount" of euxassay_015936. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_015936_01 euxassay_015936_02 euxassay_015936_03 euxassay_015936_04
EMAGE:18990 EMAGE:18990 EMAGE:18990 EMAGE:18990 EMAGE:18990
euxassay_015936_05 euxassay_015936_06 euxassay_015936_07 euxassay_015936_08 euxassay_015936_09
EMAGE:18990 EMAGE:18990 EMAGE:18990 EMAGE:18990 EMAGE:18990
euxassay_015936_10 euxassay_015936_11 euxassay_015936_12 euxassay_015936_13 euxassay_015936_14
EMAGE:18990 EMAGE:18990 EMAGE:18990 EMAGE:18990 EMAGE:18990
euxassay_015936_15 euxassay_015936_16 euxassay_015936_17 euxassay_015936_18 euxassay_015936_19
EMAGE:18990 EMAGE:18990 EMAGE:18990 EMAGE:18990 EMAGE:18990
euxassay_015936_20 euxassay_015936_21 euxassay_015936_22 euxassay_015936_23 euxassay_015936_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18990Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18990_wholemount_strong.wlz
18990_wholemount_moderate.wlz
18990_wholemount_weak.wlz
18990_wholemount_possible.wlz
18990_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18990_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
telencephalon mantle layer
weak weak
regionalweak expression: see section 12 17 18 19 20 21
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63440
Entity Detected:Tprn, taperin ( MGI:2139535)
Sequence:sense strand is shown

>T63440
CCAAGCCAGACATGGAAACTATCCCAATCGGGGACCTTCAGGCCAGGGCCCTGGCCAACCTCCGAGTGAA
CTCCCGCAACTCCTTCGTGTTGATCCCCAAGCGCAAAGCCCCTGGGAACTATCCTTTGGCGGGGAGGCAA
TTTGAGGAGCCAAAGGGGGAGGTGGGCTGGGCCTCTCAGAGCCAGGGGCTCGGATCCCAGCTGGTGTCTA
CAGTGGATGGTGCACCTGCCCTAGAGAAGAGTCCCTTGGCTGCTGAGATGCAGTGGGCAGTTAGGAAGGG
GGCCTGCCCCAGGCCAGCAATTTCTGACACAGACAAGTGTGTTAGGTGGCAGAGACCAGCCTCGCCACCT
CCATTTCTACCAGCCACAGCTGAAGCTGAGCCGGCTGAAGGCTTGGGGGTTCCTGGCTTGGCCAAGAATG
GCCAGGAGCCTGTGAGGCCAGGACTTCCAGTCACCTTCATTGATGAAGTAGACTCAGAGGAGGAGGCCTT
TCAAGAAGCCAAACTGCCCTCCTCAGCAGTTGGTGTGCCTTCCCAGTACCACCTGCACCCTGCCAGGCCT
GGACACACCTCAGAACTCCTGAACCGAGGTAGCAACACTTTCACAGTAGTGCCCAAGAGAAAGCCAGGGA
CCCTTCAGGAGCCACACTTAAGTCAGACCAATGGTCAGTCCCAGCAAGGGGCAGAGGAACAGGATGCTGA
GAGCCTCTCAGGACCCCATACTACCCTGGAGAACACCCTAAAGAAACGATACCCCACTGTGCAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 68447. Forward Primer - name:068447_F_cDNA_C430004E15Rik, sequence:CCAAGCCAGACATGGAAACTAT; Reverse Primer - name:068447_N_SP6_cDNA_C430004E15Rik, sequence:GTGCACAGTGGGGTATCGTTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18992 same embryo
 EMAGE:18991 same embryo
 EMAGE:18989 same embryo
 EurExpress:euxassay_015936 same experiment
 MGI:4823556 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS