EMAGE mouse gene expression spatial database

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EMAGE:18992

Dtx4 deltex 4 homolog (Drosophila) ( MGI:2672905)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_015898. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_015898_01	euxassay_015898_02	euxassay_015898_03	euxassay_015898_04

euxassay_015898_05	euxassay_015898_06	euxassay_015898_07	euxassay_015898_08	euxassay_015898_09

euxassay_015898_10	euxassay_015898_11	euxassay_015898_12	euxassay_015898_13	euxassay_015898_14

euxassay_015898_15	euxassay_015898_16	euxassay_015898_17	euxassay_015898_18	euxassay_015898_19

euxassay_015898_20	euxassay_015898_21	euxassay_015898_22	euxassay_015898_23	euxassay_015898_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18992_wholemount_strong.wlz

18992_wholemount_moderate.wlz

18992_wholemount_weak.wlz

18992_wholemount_possible.wlz

18992_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18992_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 13 14 16 weak expression: see section 15

medulla oblongata floor plate

weak

regional

weak expression: see section 13 14

medulla oblongata basal plate ventricular layer

weak

regional

weak expression: see section 11 12 15 16

metencephalon floor plate

moderate

regional

moderate expression: see section 14

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 05 06 moderate expression: see section 07 11 13 15 21 22 23 weak expression: see section 08 09 12 16 17 18 19 20

pons ventricular layer

moderate

regional

moderate expression: see section 09 10 11 13 15 weak expression: see section 12 16 17 18 19 20

midbrain floor plate

moderate

regional

moderate expression: see section 14

midbrain ventricular layer

moderate

regional

moderate expression: see section 10 11 13 15 weak expression: see section 12 16 17 18 19

spinal cord floor plate

moderate

regional

moderate expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T63296
Entity Detected:	Dtx4, deltex 4 homolog (Drosophila) ( MGI:2672905)
Sequence:	sense strand is shown >T63296 GTCCACATTTTCATACCACCCTGCACCAGAGATGCCTCCTGTTGGGCTTCCACCATTGTTGATATTGCTG TGGCCAGTAGAATCACCCAAGTGTCCCTAACCCACTTCCCTGGTGCTGAGTTTTCTTTCACTCTACAAAT GGCTAATGGAATTTTAATCGCCTTCCACTAGGGGGAGCTGTGGTTGGAGGGAATGTTCTGCCATCTTAGT CCAGCCTGTCCTATCAATGGGAGGCCATTTTGTCTCACAAGCAGAGCAGAAGATTGCTGCATTCACTGCA CCTCGGGGAAAACTCCTGCTAAAGTAGAGAAAGATACAGCAGAACTAAATGGAAGGCAAGTAGCCACGGG CGTTAATGTCACACCCTTTTATCAGGTTACCGAATTTCAGAGATAATCGGACCCTCATTGAAGGCTTACA ATCATGTTTGTCTTCAGTTACTCGTTGTTATCTACAGTCCCTGCTTCTTGGCAACAAGGATGAGAAATTA GACTGGGAGTTCATGGATAGGAAAGTGAAGCAGTAGGAAGTGAAGAAATGAAAACAGGCTCTGAGATGTG GGAATGGCCAGTGGGGGATGTTTTCACGTAGCAAAGGAGGTAGACGCAGGAAAAGCATAGCATGAGGCAG AAGGAAGAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 96314. Forward Primer - name:096314_F_cDNA_Dtx4, sequence:GTCCACATTTTCATACCACCCT; Reverse Primer - name:096314_N_SP6_cDNA_Dtx4, sequence:CTCTTCCTTCTGCCTCATGCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18990 same embryo
	EMAGE:18991 same embryo
	EMAGE:18989 same embryo
	EurExpress:euxassay_015898 same experiment
	MGI:4824409 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source