EMAGE mouse gene expression spatial database

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EMAGE:19351

Ggt6 gamma-glutamyltransferase 6 ( MGI:1918772)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002096. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002096_01	euxassay_002096_02	euxassay_002096_03	euxassay_002096_04

euxassay_002096_05	euxassay_002096_06	euxassay_002096_07	euxassay_002096_08	euxassay_002096_09

euxassay_002096_10	euxassay_002096_11	euxassay_002096_12	euxassay_002096_13	euxassay_002096_14

euxassay_002096_15	euxassay_002096_16	euxassay_002096_17	euxassay_002096_18	euxassay_002096_19

euxassay_002096_20	euxassay_002096_21	euxassay_002096_22	euxassay_002096_23	euxassay_002096_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19351_wholemount_strong.wlz

19351_wholemount_moderate.wlz

19351_wholemount_weak.wlz

19351_wholemount_possible.wlz

19351_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19351_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5502
Entity Detected:	Ggt6, gamma-glutamyltransferase 6 ( MGI:1918772)
Sequence:	sense strand is shown >T5502 AGGCCACCCAGCACCAGCCACAACCTGCTTGCTTGCCTCTCTGGCAGTGTCAACTGTCAGGGCTCTCGGC AAAGATCTGGGGTAGCACGGGCCATGGATGCCACCACAGGACCTGTGCACTATCACAAGCTACAACTCTG GGAGCCTGGCGTGGAGTCAGAGGAGGAGGAGGAGGAGGAAGAGGAAGAGATAGCAGAGCCACTGGTCCTC TCCCTAAGGAGACTCCAAAATACCCCCCGGAACGAGGTTGGTGGGTTGCCAGGAGCCTGGGCCCGCCTGC TGGCAGGCTTGTTGCTGCTGGCTGTTAGCAGCTCTCTGGCTCTGAGGCAGCTGCACAGTAGGGACAGCCC AAGAGGAAACTTGGGCTCTGTGGCCCCTCCAGCCAGCAGACACTCCCATCGCCCTGGTGTGTACCACCAC AGCGCCATTATCAGCCCGGCAGCCACATGTTCCCAACTGGGCCAAGAGTTGCTTGTCGCTGGGGGCAATG TCGTGGATGCTGGAGTTGGAGCAGCTTTGTGTCTGGCGGTGGTACATCCTCATGCAACAGGCTTAGGTGC CACATTCTGGGGTCTCTTCTACAATAGCTCTTCAGGAAACTCAACTGCCCTGACAGCAGGCCCAACCCAA CTCCTGGCCCCCGGCCTGGGGCTGCCCACGGGTCTGCCTGCTCTGCATTTGCTTCATGCCCACTTCGGCC GCCTGCCCTGGCCACACCTGCTGACCAAGCCTGCCATGCTGGCTGAAAAGGGCTTTGAGGTAGATGCACC CCTGGCAAACGCACTAGCCATCCAGGGCACAAAGGGGCTCTGTCCATTGTTCTGCCATACCAATGGAACC CCACTGGGCCTTGGGGCTCGGGCCACCAACCCCAACCTGGCAGCTGTATTACGCAGCGCAGCCCTGGCCT CCAGCCCAGATCTCGCTGGGAAGGCCTTGCTGAACCCGCTGGTCAGAGACCTGGGGTTAGAGTTACCTTC TGCTCAGCCCGTGCCTTCCTTAGAGCCTGCGCTGCAGCTTCTTCTGCCTCGGGGGGTCCTGTTCACGACT CCTGGCCCCTCAGCAGGTCCAGAACTTGTGGAACTGCTGGAGTCTACTCTTCACTCCAGGACACCCAGCT CTGCTCCCTGCCCACCATTCCTGCAAACTGCTGAAACCCCAGTGAGCAGTGCCCTGGCCACCGTGGACAG CAACGGCTCCATGCTCCTCCTCATCTCCTCAATCAACAGTTCCTTTGGTTCTGGACATCTATCCCCAAGT ACTGGTGTTCTGCTCAGCAACCTGGAAGCCAGCCCTGCACCTAGTGCCTGGGCCTGTCCACTCATCCTTC GTGACAACCTGGATGACACAGAAGCCGATATGTTGGGGATGGTGGCTTCAGGGATCTCCAGAGGGGCCAA AGCCATGACTTGCACCTTGCTCAATCATCTGGCAACACCCCAAATCCCACAGCAGCCCCAGCATCAAAGA CCCACAGAGAGCCCTGGCATATGTGGCCAAGGGGCCCTACTGCAGGCGGTAGTCCATGCAGAACACGCCC ATGTCTCCAGTGTCCCCAGTGGCTGCTGCCCCTTTCAGGGGTATTAACAGAGGGGCAGAGGTGTCTGGAA CTTGAGAGCCAGACAGAGAAGGTCTAGCAGCAATGAATGTATGAAGAGAATGTATCTGTGAGGTGTTTGC TGCTCCATCACATGAAGCCTCCATCCTAATCTGTTACCTGGCTCTGGCTCTGGCATCCAGGGCTGGTCCA ACCACATTTCCTGGTACCCACACTGATGGAATTTCTGACTTTTCTATCAAATAAAGACCAGAAGATGATG AAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6394797 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6394797 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19352 same embryo
	EMAGE:19350 same embryo
	EMAGE:19353 same embryo
	EMAGE:19349 same embryo
	EMAGE:19348 same embryo
	EurExpress:euxassay_002096 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source