EMAGE mouse gene expression spatial database

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EMAGE:19352

2810405K02Rik RIKEN cDNA 2810405K02 gene ( MGI:1913719)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002095. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002095_01	euxassay_002095_02	euxassay_002095_03	euxassay_002095_04

euxassay_002095_05	euxassay_002095_06	euxassay_002095_07	euxassay_002095_08	euxassay_002095_09

euxassay_002095_10	euxassay_002095_11	euxassay_002095_12	euxassay_002095_13	euxassay_002095_14

euxassay_002095_15	euxassay_002095_16	euxassay_002095_17	euxassay_002095_18	euxassay_002095_19

euxassay_002095_20	euxassay_002095_21	euxassay_002095_22	euxassay_002095_23	euxassay_002095_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19352_wholemount_strong.wlz

19352_wholemount_moderate.wlz

19352_wholemount_weak.wlz

19352_wholemount_possible.wlz

19352_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19352_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5499
Entity Detected:	2810405K02Rik, RIKEN cDNA 2810405K02 gene ( MGI:1913719)
Sequence:	sense strand is shown >T5499 CCACGCGTCCGCAGAAAGGGACGGAGGAGGCCATGAATGTGGTGGACCTTGGTCGCGTAGGAGCGTGTGT CTTGAAACATGCAGTGACTGGGGAGGCTGTGGAGCTGCGGAGCCTATGGCAGGAGAAAGCTTGTGTGGTG GCCGGTCTGCGACGCTTCGGCTGCATGGTGTGCCGCTGGATCGCCCAGGACCTCAGCAACCTCCGGAGCA TCCTGGACCAACACGATGTGCGCCTGGTGGGCGTGGGGCCTGAGGCCCTGGGCCTGCAGGAGTTTCTGGA TGGTGGTTACTTCTCAGGAGAACTCTACCTTGATGAGAGCAAGCAAATCTATAAGGAGCTGGGCTTCAAG CGGTACAACAGCTTAAGCATCCTACCAGCTGCCCTGGGAAAACCTGTTCGTGATGTAGCCTCCAAGGCTA AAGCTGTTGGTATCCAGGGGAACCTGTCTGGTGACCTGCTGCAAAGTGGAGGGCTGCTGGTGGTCAGCAA GGGTGGCGACAAGGTACTGTTGCACTTCATCCAGAAGTCCCCGGGTGACTATGTTCCCCAGGAGAACATC CTGCAGGCCTTGGGTATCTCTGCAGAGGTTTGCTCCAGCAAGCCACCCCAGTGTGATGAAGAGGTGTGTG GGAGGTGACACGCACAGCCTGAGGACCCGGAGGCATCTGTAACCTTGGATGTGTTTGGAACACAAAGAAT CCCTGTGGAGCCACTGAGCCAGGACGTTGGACCTTGTTCATCTTTCAACAGCAATGAGCCATTAAAGTGC AGGTCCTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5401948 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5401948 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19351 same embryo
	EMAGE:19350 same embryo
	EMAGE:19353 same embryo
	EMAGE:19349 same embryo
	EMAGE:19348 same embryo
	EurExpress:euxassay_002095 same experiment
	MGI:4822687 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source