EMAGE mouse gene expression spatial database

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EMAGE:19373

Mecr mitochondrial trans-2-enoyl-CoA reductase ( MGI:1349441)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002169. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002169_01	euxassay_002169_02	euxassay_002169_03	euxassay_002169_04

euxassay_002169_05	euxassay_002169_06	euxassay_002169_07	euxassay_002169_08	euxassay_002169_09

euxassay_002169_10	euxassay_002169_11	euxassay_002169_12	euxassay_002169_13	euxassay_002169_14

euxassay_002169_15	euxassay_002169_16	euxassay_002169_17	euxassay_002169_18	euxassay_002169_19

euxassay_002169_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19373_wholemount_strong.wlz

19373_wholemount_moderate.wlz

19373_wholemount_weak.wlz

19373_wholemount_possible.wlz

19373_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19373_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 05 06 07 08 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6034
Entity Detected:	Mecr, mitochondrial trans-2-enoyl-CoA reductase ( MGI:1349441)
Sequence:	sense strand is shown >T6034 CGGACGCGTGGGCGAACGCGTGGGGAAGCAGCATGTTGGTCAGCCAGCGAGTGACAGGGGCGCGAGCCCG GGCACCTCAGCTTGCGGGTCTCCTCGAGGCTTGGTACCGCCACGGCCGGACCACATCCTCCTACTCCGCT CTCTCTGAGCCGTCACGGGTGCGGGCGCTGGTCTATGGCAACCATGGGGATCCAGCCAAGGTCGTCCAGC TGAAGAACCTGGAGCTCACTGCTGTGGAAGGATCTGACGTCCACGTGAGGATGCTGGCAGCCCCTATCAA TCCATCTGACATAAATATGATCCAAGGGAACTATGGCCTCCTTCCCAAGCTGCCTGCTGTTGGAGGGAAC GAAGGCGTTGGGCAGGTGATAGCGGTGGGCAGCAGTGTGTCTGCATTGAAGCCAGGAGATTGGGTGATCC CTGCAAATGCGGGTTTGGGAACCTGGCGGACTGAGGCGGTGTTCAGTGAGGAAGCACTGATTGGAATCCC TAAGGACATTCCTCTCCAGAGCGCTGCCACCCTAGGTGTCAACCCCTGCACAGCCTACAGGATGTTGGTG GACTTTGAACAGCTGCAGCCAGGGGACTCTGTCATCCAGAATGCATCCAACAGTGGAGTGGGCCAAGCAG TCATTCAGATCGCCTCAGCCCTTCGCCTAAAGACCATCAACGTTGTCCGAGACAGACCCGACATCAAGAA GCTAACTGACAGACTGAAGGATCTAGGAGCTGATTATGTCCTCACAGAGGAAGAGCTAAGGATGCCCGAG ACAAAAACCATCTTCAAGGACCTGCCGCTGCCCCGATTGGCTCTCAACTGTGTTGGTGGGAAGAGTTCTA CAGAGCTGCTCCGGCACCTAGCGCCCGGAGGAACCATGGTGACCTATGGGGGAATGGCCAAACAGCCTGT AACAGCCTCTGTGAGTCTGCTCATTTTTAAGGACCTCAAACTTCGGGGCTTTTGGTTGTCCCAGTGGAAG AAGAACCACAGTCCAGATGAGTTCAAGGAGCTGATTCTCACTCTCTGCAACCTCATCCGCCAAGGCCGGC TCACAGCCCCATCCTGTTCTGAGGTTCCACTGCAGGGCTACCAGCAGGCTTTGGAAGCCTCCATGAAGCC TTTTGTGTCTTCGAAGCAGATTCTCACCATGTGATTGCTCCAGAGGACCAGGAGGAAAGCAGGAGAGGCA AGACTGGCTGTCTGCTGGCCCCTCCATGAGAACCCTAGCCTTCCCAGACTGCCTCACCCATATTGTCTCT TCCTACCAGGAGGGTGGGGGACCAACTCTAGGCTCCCTAATAAACCCTTAACTTCCCGAAAAAAAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3488760 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3488760 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19376 same embryo
	EMAGE:19375 same embryo
	EMAGE:19374 same embryo
	EMAGE:19372 same embryo
	EMAGE:19371 same embryo
	EurExpress:euxassay_002169 same experiment
	MGI:4826149 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source