EMAGE mouse gene expression spatial database

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EMAGE:19403

Ppp4c protein phosphatase 4, catalytic subunit ( MGI:1891763)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004804. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004804_01	euxassay_004804_02	euxassay_004804_03	euxassay_004804_04

euxassay_004804_05	euxassay_004804_06	euxassay_004804_07	euxassay_004804_08	euxassay_004804_09

euxassay_004804_10	euxassay_004804_11	euxassay_004804_12	euxassay_004804_13	euxassay_004804_14

euxassay_004804_15	euxassay_004804_16	euxassay_004804_17	euxassay_004804_18	euxassay_004804_19

euxassay_004804_20	euxassay_004804_21	euxassay_004804_22	euxassay_004804_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19403_wholemount_strong.wlz

19403_wholemount_moderate.wlz

19403_wholemount_weak.wlz

19403_wholemount_possible.wlz

19403_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19403_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 09 10 13 weak expression: see section 11 12

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 09 10 14 15

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 01 02 03 05 06 07 08 09 10 12 13 14 15 16 17 18 19 20 21 weak expression: see section 04

midbrain ventricular layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 weak expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6073
Entity Detected:	Ppp4c, protein phosphatase 4, catalytic subunit ( MGI:1891763)
Sequence:	sense strand is shown >T6073 GCGGAGGGAAGTGGGAGTCGAAGAGACTCAGCCGGAGTCTCCGCGGCGGAGACCCCTGTGCGGTCCGGAG GGGGCGGCGGCCCCGACTCTGACCCGCGCCGGGGGGGGTGGGCCATGGCGGAGATCAGCGACCTGGACCG GCAGATCGAGCAGCTGCGGCGCTGCGAGCTGATCAAAGAGAGTGAAGTCAAGGCCCTGTGCGCCAAGGCC AGAGAAATCTTGGTAGAAGAGAGCAACGTGCAGAGGGTGGACTCGCCAGTCACAGTATGCGGTGACATCC ATGGACAATTCTATGACCTCAAGGAGCTGTTCAGAGTAGGTGGCGATGTCCCTGAGACCAACTACCTCTT CATGGGAGACTTTGTGGACCGTGGTTTCTACAGTGTTGAAACCTTCCTCCTCCTGCTGGCTCTTAAGGTT CGCTATCCTGACAGAATCACTTTGATCCGGGGCAATCATGAGAGTCGCCAGATTACCCAGGTCTATGGGT TCTACGATGAGTGCTTACGGAAATATGGTTCAGTGACTGTATGGCGCTACTGTACTGAGATCTTTGACTA CCTCAGCCTGTCTGCCATCATTGATGGCAAGATCTTCTGTGTGCATGGAGGTCTTTCCCCTTCCATCCAG ACCTTGGACCAGATCCGGACAATTGACCGAAAGCAAGAGGTACCCCATGATGGACCCATGTGCGACCTCC TGTGGTCTGACCCTGAAGACACAACAGGCTGGGGAGTGAGCCCCCGCGGGGCAGGTTACCTGTTTGGCAG TGACGTGGTCGCCCAGTTCAATGCAGCCAACGACATTGATATGATCTGCCGTGCCCACCAATTAGTGATG GAAGGCTACAAGTGGCACTTCAATGAGACCGTGCTTACTGTGTGGTCAGCGCCTAATTACTGCTACCGCT GTGGCAATGTGGCAGCCATCTTAGAACTGGATGAGCACCTCCAGAAAGATTTCATCATCTTCGAGGCTGC ACCCCAAGAGACACGTGGCATCCCCTCCAAAAAGCCAGTGGCCGACTATTTCCTGTGACTCCTTGGCCCC AGCCCCTCTGGCCCTTGGACCACTGTGACTGCCCTCTGCCCAGTCGGAAGCTGGGTCTGAGGGGCTGCCT GGCTTTGCTTGCCCCAGGGTGCGGACTTGCTCTGGAGAGGTGGAGCCTTGGCTCCTGGCTTCCTCTCCTT TCTCCCACTTGATCCATGAAGTTTTCAGTCATTTTTTTTTCTTTTTTCCTTTTTTTTCCTTTCTTTTTTC TTTTTGTTTTTGTTTTTAGATAAAACATTTTGAGAAAAAAAGAAAAAAATTCTAATAAAAGAAGAAAAAT AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3491568 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3491568 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19402 same embryo
	EMAGE:19405 same embryo
	EMAGE:19404 same embryo
	EMAGE:19401 same embryo
	EMAGE:19406 same embryo
	EurExpress:euxassay_004804 same experiment
	MGI:4827389 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source