EMAGE mouse gene expression spatial database

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EMAGE:19434

Yme1l1 YME1-like 1 (S. cerevisiae) ( MGI:1351651)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004836. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004836_01	euxassay_004836_02	euxassay_004836_03	euxassay_004836_04

euxassay_004836_05	euxassay_004836_06	euxassay_004836_07	euxassay_004836_08	euxassay_004836_09

euxassay_004836_10	euxassay_004836_11	euxassay_004836_12	euxassay_004836_13	euxassay_004836_14

euxassay_004836_15	euxassay_004836_16	euxassay_004836_17	euxassay_004836_18	euxassay_004836_19

euxassay_004836_20	euxassay_004836_21	euxassay_004836_22	euxassay_004836_23	euxassay_004836_24

euxassay_004836_25	euxassay_004836_26	euxassay_004836_27	euxassay_004836_28

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19434_wholemount_strong.wlz

19434_wholemount_moderate.wlz

19434_wholemount_weak.wlz

19434_wholemount_possible.wlz

19434_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19434_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6533
Entity Detected:	Yme1l1, YME1-like 1 (S. cerevisiae) ( MGI:1351651)
Sequence:	sense strand is shown >T6533 GGGGAAGCCATTTTTTCGTTTCTGGGAGGAGTAAGGGAGAAACTGGGAGAAAAGCAAAGAGAAATAAAGA GAAAATAAAAGGGCTCAGCCCCTTTCCTCGGAGCTGAAGCTAAGAAGCAGTTTCGGCTGGCCGGTAAGAT CTCGAGGGCCCGGAGATGTTCTCCCTGTCGAGCACTGTGCAGCCTCAGGTTACAATTCCTCTCAGTCATC TCATCAATGCTTTCCATTCACCAAAAAATATATCTGTTTCTGTCAATACACCTGTTTCTCAAAAACAGCA TCGAGATACAGTTCCTGAGCATGAAGCTCCCAGTAGTGAGCCTGTGCTTAATTTAAGGGACCTTGGATTA TCTGAATTGAAAATTGGACAGATTGATAAAATGGTAGAAAATTTACTTCCTGGGTTTTATAAAGACAAAA GAGTTTCTTCCTGTTGGCATACATCTCATATTTCGGCACAGTCCTTTTTTGAAAATAAATATGGTCACTT AGATATGTTCAGTACATTACGTTCCTCTAGCTTGTACAGGCAACATCCAAAAACTCTTCGAAGCATTTGT TCAGATCTTCAGTATTTTCCAGTTTTCATACAGTCTCGGGGTTTCAAAACGTTGAAATCAAGGACACGAC GTTTGCAGTCTACCTCTGAAAGATTAGTAGAAGCACAGAATATAGCACCATCATTTGTGAAGGGGTTTCT TTTGCGGGACAGAGGAACAGATCTTGAGAGTTTGGACAAACTTATGAAAACTAAAAACATACCTGAAGCT CACCAAGATGCATTTAAAACTGGTTTTGCAGAGGGTTTTCTCAAAGCTCAAGCTCTTACACAGAAGACCA ATGATTCCTTAAGGCGAACTCGTCTGATCCTCTTTGTTTTGCTCCTGTTTGGCATTTATGGACTCTTAAA AAATCCGTTTTTATCTGTGCGCTTCCGGACAACTACAGGACTTGATTCTGCGGTAGACCCTGTCCAGATG AAAAATGTCACTTTTGAACATGTTAAAGGGGTGGAGGAAGCCAAACAAGAGTTACAGGAAGTGGTTGAAT TCTTGAAAAATCCACAGAAGTTTACTGTGCTTGGAGGTAAACTTCCCAAAGGAATTCTTTTAGTTGGGCC ACCAGGAACAGGGAAGACGCTTCTTGCCCGAGCTGTGGCAGGAGAAGCTGACGTTCCTTTTTATTATGCT TCTGGATCAGAGTTTGATGAGATGTTTGTGGGCGTAGGAGCCAGCAGGATCAGAAATCTTTTTAGAGAAG CAAAAGCAAATGCTCCTTGTGTTATATTCATCGATGAATTAGATTCTGTTGGTGGAAAGAGAATTGAATC TCCAATGCACCCGTATTCAAGGCAGACGATCAATCAGCTTCTTGCTGAAATGGATGGTTTCAAACCCAAT GAAGGAGTAATCATTATAGGTGCCACAAATTTCCCAGAGGCATTAGATAATGCCTTAATACGTCCTGGTC GTTTTGATATGCAAGTTACAGTTCCAAGGCCAGATGTGAAGGGTCGAACTGAAATTTTGAAATGGTATCT CAACAAGATAAAGTTTGATAAATCTGTTGATCCAGAAATCATAGCTCGAGGGACTGTTGGGTTCTCTGGA GCAGAGTTGGAGAATCTTGTGAACCAAGCTGCACTAAAGGCAGCAGTTGATGGAAAAGAAATGGTTACCA TGAAGGAACTAGAGTTTTCCAAGGATAAAATTCTAATGGGGCCAGAAAGAAGAAGTGTGGAAATTGATAA CAAAAACAAAACTATAACAGCCTATCATGAATCTGGTCATGCTATTATTGCATATTACACAAAGGATGCA ATGCCAATTAATAAAGCTACAATCATGCCTCGAGGGCCAACACTTGGGCATGTATCACTGTTGCCTGAGA ATGACAGATGGAATGAAACTCGAGCCCAGCTGCTTGCACAGATGGATGTTAGCATGGGAGGAAGAGTGGC AGAGGAACTCATATTTGGAACTGATCATATTACAACTGGTGCTTCTAGTGATTTTGATAATGCAACAAAA ATTGCTAAGAGGATGGTTACCAAATTTGGAATGAGTGAAAAGCTTGGAGTTATGACCTACAGTGATACAG GAAAACTAAGTCCTGAAACTCAATCAGCCATTGAACAAGAAATAAGAATCCTTCTACGGGAGTCATATGA ACGAGCAAAACATATCTTGAAAACACATGCGAAAGAACATAAGAACCTGGCAGAAGCATTGCTGACCTAT GAGACTTTGGATGCCAAAGAGATTCAAATTGTTCTTGAGGGGAAGAAATTGGAAGTGAGATGATAACTCT GTTGATATTGCTGGTTTTATTACAAGAATATACTTAGTCCTGCAGTCTCCTTTGCAACATTTTTCAACCC CCACTCCTGACTTGGTATATAATTCAAGGATGTGAAACACTTTGTCAATCTTCTGTCCCATTATTTGATT TTGGTTATTCCTGTGATGATACCATTGGAGATTAGTAACTCATAGCAAATACGTGAAAGGATAAATAAAG AACTGTCAGGATTAAAGAAAGACATTGAAAAATGTCAATCAATCATGTTGAAAGCAAGCAATCATTTTGT GGGTAATTACTCCAAGTGAGTAAAAATTGTGCCTTTATCTATTGGTAAGTTTTTTTTTTTTTTTTGGAAG GTAAATTTGAAAAGCTACCATCTCCTGATAGTAACATTTCTCTGTGGGGAAAACAATGCTGTGGATAAAT CATTTCTACTTACATTAGTAGAAATGCCATTGAGATGTACTTAATTATGTTCTTGATTGACCTTGTAAAA TGGCCTTTAAAAGAAACTATTTGAATTGTAAATATGACGATATACTCAGAATTTAAATGATTGTAATGGA TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3591123 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3591123 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19439 same embryo
	EMAGE:19435 same embryo
	EMAGE:19436 same embryo
	EMAGE:19438 same embryo
	EMAGE:19437 same embryo
	EurExpress:euxassay_004836 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source