EMAGE mouse gene expression spatial database

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EMAGE:19461

Dpp8 dipeptidylpeptidase 8 ( MGI:1921638)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005146. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005146_01	euxassay_005146_02	euxassay_005146_03	euxassay_005146_04

euxassay_005146_05	euxassay_005146_06	euxassay_005146_07	euxassay_005146_08	euxassay_005146_09

euxassay_005146_10	euxassay_005146_11	euxassay_005146_12	euxassay_005146_13	euxassay_005146_14

euxassay_005146_15	euxassay_005146_16	euxassay_005146_17	euxassay_005146_18	euxassay_005146_19

euxassay_005146_20	euxassay_005146_21	euxassay_005146_22	euxassay_005146_23	euxassay_005146_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19461_wholemount_strong.wlz

19461_wholemount_moderate.wlz

19461_wholemount_weak.wlz

19461_wholemount_possible.wlz

19461_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19461_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8825
Entity Detected:	Dpp8, dipeptidylpeptidase 8 ( MGI:1921638)
Sequence:	sense strand is shown >T8825 TTCGCCTCCGCCGCTTTTAAGTCCCTTCGTCCGCTGCCTGGGGGCCGCGGGGGCCGCCGCCGAGGAAGCT GCTGTGGCCCCGACGGGAGCGGAGGCGGCGCGGCATGAAGCGGCGTAGGCCCGCTCCATAGCGCATCCGG ACGGTCCGGGCGGGCCCGGGGGGAAGGATTGGATGGTGAGAAAAGGAAAGATTCCTGTGAGAAGAGATCA GGATGAACGGAGAGATCTGTGCTTAAAGTTGGGCCAGGTGAAAATGATCTCTTTGAAAGTAGAAGAGATC TGAGCAGATGAAAATACCATCAGGAAGATGCAACATGGCAGCAGCAATGGAAACAGAACAGCTGGGTGTC GAGATATTTGAAACTGCAGAGTGTGAGGAGGGAAATGGTGAATCACAGGACCGACCCAAACTGGAGCCTT TTTATGTCGAACGGTATTCTTGGAGTCAGCTGAAAAAGCTGCTTGCTGATACCAGAAAATACCACGGCTA CATGATGGCTAAGGCGCCACATGACTTTATGTTTGTAAAGAGGACGGATCCAGATGGGCCTCACTCAGAC AGGGTCTATTACCTTGCCATGTCTGGTGAGAACAGAGAAAATACACTATTTTATTCTGAAATCCCTAAAA CCATCAACAGAGCAGCAGTCTTAATGCTTTCCTGGAAGCCCCTTTTGGATCTTTTTCAGGCGACACTAGA CTATGGGATGTATTCTCGAGAGGAAGAGCTACTCAGAGAAAGAAAGCGCATTGGAACCGTGGGGATCGCA GCTTATGATTACCACCCAGGAAGCGGAACATTCCTGTTTCAAGCTGGCAGCGGCATTTACCATATCAAAG ATGGAGGACCACATGGATTTACACAACAGCCTTTGCGGCCCAATTTAGTGGAAACTAGTTGTCCCAATAT ACGAATGGATCCAAAATTATGCCCTGCTGATCCAGACTGGATAGCTTTCATTCACAGCAATGATATTTGG ATATCAAACCTCGTAACCAGGGAAGAGCGGAGGATCACATACGTACACAATGAGCTAGCCAACATGGAAG AGGATCCCAGATCAGCTGGTGTTGCCACCTTTGTTCTTCAAGAAGAATTTGACAGATACTCTGGCTACTG GTGGTGTCCCCAAGCGGAAAGAACTCCTAGTGGTGGTAAAATTCTTAGAATTCTCTATGAAGAAAATGAT GAATCTGAGGTGGAGATTATTCATGTTACGTCCCCCATGTTGGAAACAAGGAGGGCAGATTCCTTTCGTT ATCCCAAAACAGGCACAGCAAACCCAAAGGTCACTTTCAAGATGTCGGAGATTGTTGTTGATGCTGCAGG AGGGATTATAGATGTCATAGATAAAGAACTGGTTCAACCTTTCGAGATTCTGTTTGAGGGAGTTGAATAT ATTGCCAGAGCCGGATGGACTCCAGAGGGAAAACATGCCTGGTCCATCCTACTAGACCGTTCCCAGACTC ACCTGCAGATAGTTCTGATCTCCCCTGAGTTATTCATCCCAGTAGAAGATGATGCCATGGACAGACAGAG ACTTATAGAGTCAGTTCCTGACTCTGTGACACCACTGATCATCTATGAAGAAACAACAGACATCTGGATA AATATCCACGATATTTTTCATGTTTTTCCTCAAACTCATGAAGATGAAATTGAGTTTATTTTTGCCTCTG AATGCAAAACAGGTTTTCGTCATCTGTATAAAATCACATCCATTTTAAAGGAGAGCAAATATAAACGGTC CAGTGGTGGACTACCTGCCCCAAGTGATTTCAAGTGTCCTATCAAAGAAGAAATAACAATTACCAGTGGT GAATGGGAAGTACTTGGCCGGCATGGATCTAATATCTGGGTTGATGAAGCCAGAAAGCTGGTGTACTTTG AAGGCACCAAAGACTCTCCTTTGGAGCATCACCTGTACGTGACCAGTTATGCAAACCCTGGAGAAGTGGT GAGGCTGACTGACCGTGGCTACTCACACTCCTGCTGCCTCAGCCGGCATTGTGACTTCTTCATAAGTAAG TACAGCAACCAGAAGAATCCACACTGTGTGTCCCTCTACAAACTCTCAAGTCCTGAGGATGACCCAGTTC ATAAAACAAAGGAATTTTGGGCCACCATTTTGGATTCAGCAGGTCCTCTTCCTGACTACACCCCTCCAGA AATTTTTTCTTTTGAAAGTACTACTGGATTTACACTGTATGGAATGTTGTATAAGCCTCATGACCTACAA CCTGGAAAGAAATACCCCACTGTGTTATTCATATATGGTGGTCCCCAGGTGCAGCTGGTGAACAATCGGT TTAAAGGAGTCAAGTATTTCCGCCTGAACACCCTGGCCTCCCTGGGTTATGTGGTTGTGGTGATAGACAA CAGGGGATCCTGTCACCGAGGACTTAAATTTGAAGGCGCCTTTAAATATAAAATGGGTCAAATAGAAATC GATGATCAAGTGGAAGGACTCCAGTACCTAGCATCTCAGTATGACTTCATTGACTTGGATCGAGTGGGCA TCCACGGCTGGTCCTATGGTGGCTACCTCTCCCTGATGGCATTAATGCAGAGGTCGGATATCTTCCGGGT GGCTATTGCTGGGGCCCCAGTCACCCTGTGGATCTTCTATGATACAGGATACACGGAGCGCTATATGGGT CACCCTGACCAGAATGAACAGGGCTACTACTTAGGATCTGTGGCCATGCAAGCGGAGAAGTTCCCCTCAG AACCAAACCGGTTACTCCTCTTGCATGGGTTCTTGGATGAGAATGTTCACTTTGCACACACCAGTATATT GCTGAGTTTTTTAGTGAGGGCTGGAAAGCCATATGACTTACAGATCTATCCTCAGGAGAGGCACAGCATC AGAGTTCCTGAGTCTGGAGAACACTATGAACTGCACCTGCTCCACTACCTTCAGGAGAACCTTGGATCGC GTATCGCTGCTCTGAAAGTGATATAACTCTGACGCATGTAGAACTTGGTGTACACTGTCTAACCAAACAA AGAGGGTTAGCCAATAGAGAAGCCAGAGTTGATGAACCTACTTTTGTACCTGCCACATAAGATCTATTTC TGAAAGTAAATTTGGTGCCATGCAGAAATCTGCAGTTCATGGATAGTACTCTAATACCTTAACCATGCAC ATAAAACTGAGTGACATGTTCCCAAAGGATTGAACAGTACCTGAGGATACTGAAGGAATCAAGGCACCAG CGCCACATATTCAGACTAGCCTACTTTCACTTTACTAGCAGTATAAGCCTCTTGGACTTAATTAGTGGAC TTTACAGTATACTCTGAGTTTGTTAAAATATGATGATAGTAGTGATTGGTTTGGTTCAATTCCAGGATCT CTGACTAGTTATGGATTTGATAGCACTTACGTCTAATTGAATAGCTTATGCTTCGTCACTTGGGGTGTAT CCAGCATGTCATGAACTAAATACTATTAAGCTTATGACTTTCCCAGTCATTAATAATTTTTCAAGGATAA CCTAGTGGCCTCCTAAAGATACTTACCTGGCTGGTCTCTGACCAGTTTTTTAACCAATTTAAACAGCATC TAGTCTAAGTAATTCTCCTTTTGCTTTGATGATGTGATGGAGTGGGGTTTTCTTTTACATAAAGGCAAGT AACTATGTAGCATGAGTTTAATCACAAGTCTCAAGATGTTGACAATTATAAGGAAAACATTTTTAATCAA ATTATTTGAAAGCTTGAAAATTAGCAGGCAAGTTTTTTCTCCTTTTAGGAACAGAACATGTACACATTCA CATGTATTCTGAAAATATAATGGTGCCGTTTCCATCTGGCCTTTCCTTAATTAAAGTGTTCCCTAGTTTC CAGGAGGTAAAAGGGTTACAGTGGGAAGGGGGAGACTCTAGTGCAGGGAATGACCAGGAAAGACCAGGTA GCAAGGCTCTGCTTCACCATAAATACTCATGAGAAAACCCAGGGTAAATGTAACCCCCGAGTGGCTGGAA ACCAGAGCTAGGAGGTCAAGCTTTGACAGCTACAGGGTCAGTCAGTCTTTGGAGTGGTTACTTTTATAAT TCCAGCTTTTTGTTATTTTTCCTTTTAACTTTAAAAACCTTACAAATCTAAACTTTAACTTTTTTTTATT TGAATGTAATTTAGATATCACTAGCTTAAAAGAAAAAGGTTTCCAGACCACTTCTTGCGGTAAGCTGATC CCTGACAAACCATGGCACTCTTTGTGTTGGAAGCCTATCAAGTTAGTTGTGTTACTCGCTCACAGCATTC TCCTGCTTCAGCCTTCCTGCTTGACTATGAAGGGAAGCATGAGCTTTCAAGTGCTGGCTGGCATCTGCCC TTCCAGTATTAGATGGGTCCCACTGCAGCTAGCCAGACACCTGTGGGTTTGACCCTCATTAATCACACCC CAGTGTTTAGACAGTTACCACAACTTAAAAATCACTCCAAGAAAAGTTGCTGTGTTTCTTTCTCACTGCC ACATCAGGTCCCTTTCCAAGAGAGCACAGTGAAGACAGGGACTGCCAGAATAGAGGTGGATGCTTTGGGA AGCCTGGAGATAAATGGCAGGTGACCATGGGCTTCTGTCCTGGGGAGGGAGGGCTGGGTTTGCCAGCTGT GTGTCTATGTTGTATGATAGACCCCACACTTTTCTTTTTGTTTATCTATTTTGACTTTCATGCTTATGAC ATTTTCTCGTGCCAAAATTTGTTTATATTTTTTCAAGTTAAATTAAACTGATTTGGGTAACTTTCCTTCC CCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6410075 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6410075 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19459 same embryo
	EMAGE:19463 same embryo
	EMAGE:19458 same embryo
	EMAGE:19460 same embryo
	EMAGE:19462 same embryo
	EurExpress:euxassay_005146 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source