EMAGE mouse gene expression spatial database

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EMAGE:19462

Pdhx pyruvate dehydrogenase complex, component X ( MGI:1351627)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005192. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005192_01	euxassay_005192_02	euxassay_005192_03	euxassay_005192_04

euxassay_005192_05	euxassay_005192_06	euxassay_005192_07	euxassay_005192_08	euxassay_005192_09

euxassay_005192_10	euxassay_005192_11	euxassay_005192_12	euxassay_005192_13	euxassay_005192_14

euxassay_005192_15	euxassay_005192_16	euxassay_005192_17	euxassay_005192_18	euxassay_005192_19

euxassay_005192_20	euxassay_005192_21	euxassay_005192_22	euxassay_005192_23	euxassay_005192_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19462_wholemount_strong.wlz

19462_wholemount_moderate.wlz

19462_wholemount_weak.wlz

19462_wholemount_possible.wlz

19462_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19462_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9622
Entity Detected:	Pdhx, pyruvate dehydrogenase complex, component X ( MGI:1351627)
Sequence:	sense strand is shown >T9622 AGCTTTCCCGGAGAAAGCCGGGAAGATGGCGGCCTCCTGGAGGCTTCACTGTAACCAGCCGCTGCTTCGG TACCTTCTGGGATTTAGCAGCCGTCGAAGCCTGGGACTGGCTCAGGGTGCTGCTGCTTGGCCTGTCGACC GTGGAGCAAGTTGGAGGTGGTTTCACAGTACTCAGCTGCTGCAGGCGGATCCTATTAAGGTCCTAATGCC ATCACTGTCTCCTACGATGGAGCAAGGGAACATTGTGAAATGGCTGAGAAAGGAAGGTGAAGCTGTGAGT GCTGGAGACTCGTTATGTGAAATTGAGACGGACAAGGCTGTGGTCACCTTAGATGCAAACGATGATGGCA TCTTAGCCAAGATAGTGGTTGAGGAAGGTGCTAAAAATATACAGCTGGGTTCACTCATTGCTTTGATGGT TGAAGAAGGGGAAGATTGGAAACAGGTGGAAATTCCCAAGGATGTCAGTGCTCCACCTCCAGTCTCAAAA CCACCAGCTCCTACTCAGCCCTCTCCACAGCCGCAGATTCCTTGTCCCGCCAGGAAGGAGCACAAAGGGA CGGCACGGTTCCGCTTAAGTCCAGCTGCCCGCAACATTCTGGAAAAACATTCATTGGACGCCAGCCAGGG CACAGCCACAGGCCCTCGCGGGATCTTCACTAAAGAAGATGCACTCAAGTTAGTCGAGCTGAAACAGATG GGCAAGATTACTGAATCCAGACCAGCCTCAGCTCCTCCACCCTCTCTCTCAGCATCTGTGCCTCCTCAGG CTACAGCCGGGCCATCCTATCCTCGGCCAATGACCCCACCAGTATCCATTCCTGGGCAACCGAATGCAGC GGGTACATTCACCGAAATTCCTGCTAGCAATATTCGAAGAGTTATCGCTAAGAGGTTAACTGAATCAAAA AGTACTGTGCCTCATGCCTATGCTACTGCTGACTGTGACCTTGGCGCAGTTCTAAAGGTTAGACGAGATC TGGTCAAAGATGACATTAAAGTCTCAGTAAATGACTTTATCATCAGAGCAGCAGCTGTTACTCTTAAACA AATGCCTGGGGTTAATGTCACCTGGGATGGAGAAGGCCCAAAGCAGCTGCCCTCTGTTGACATTTCAGTG GCTGTGGCAACAGATAAAGGTTTAATTACACCAATCATAAAAGATGCAGCCGCTAAGGGCATTCAGGAGA TCGCTGACTCTGTAAAGGTTCTGTCGAAGAAAGCACGGGATGGGAAATTGATGCCAGAGGAGTACCAAGG AGGATCTTTTAGTATTTCCAACTTGGGGATGTTTGGCATCGATGAGTTCGCTGCAGTGATTAACCCGCCT CAGGCCTGTATCTTGGCCGTTGGGAGATTCCGCCCCGTGCTGAAGCTGACGGAGGATGAAGAGGGGAACC CTCAACTGCAGCAGCACCAGCTTATAACGGTCACAATGTCAAGTGACAGCCGAGTGGTTGATGATGAACT AGCTACCAGGTTTCTTGAAACTTTCAAAGCAAACCTAGAGAATCCCATGCGACTTGGGTAGCCTTCAGAG ACCAGGCGCTTGATTGGGTGTTCAGTATGGAGTAGATGAAGTGCGTATTTATTTAAGGCGAAAGCTTTGA CCTGAGCTGTCTTCCTCTCCCCTGTACATGTACTGTAGAAATGTGTGATGACAAACGGACTAATAAAGAA CATTTGGTTGGTCAGATCCACTTTTAACCTCTGGTGCTGTACAGAAGGCATTAAACTAATGTAAACAAAA AAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6532760 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6532760 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19461 same embryo
	EMAGE:19459 same embryo
	EMAGE:19463 same embryo
	EMAGE:19458 same embryo
	EMAGE:19460 same embryo
	EurExpress:euxassay_005192 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source