EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19521

Asnsd1 asparagine synthetase domain containing 1 ( MGI:1917646)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004908. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004908_01	euxassay_004908_02	euxassay_004908_03	euxassay_004908_04

euxassay_004908_05	euxassay_004908_06	euxassay_004908_07	euxassay_004908_08	euxassay_004908_09

euxassay_004908_10	euxassay_004908_11	euxassay_004908_12	euxassay_004908_13	euxassay_004908_14

euxassay_004908_15	euxassay_004908_16	euxassay_004908_17	euxassay_004908_18	euxassay_004908_19

euxassay_004908_20	euxassay_004908_21	euxassay_004908_22	euxassay_004908_23	euxassay_004908_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19521_wholemount_strong.wlz

19521_wholemount_moderate.wlz

19521_wholemount_weak.wlz

19521_wholemount_possible.wlz

19521_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19521_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

body cavity or lining

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

limb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 23 24 not examined expression: see section 15 16

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

organ system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

tail

moderate

regional

moderate expression: see section 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6910
Entity Detected:	Asnsd1, asparagine synthetase domain containing 1 ( MGI:1917646)
Sequence:	sense strand is shown >T6910 CCCACACACATCGACTGCGCACGCCGAGTCCCTTAGCAGCAAGATTAAAGAACAGAAAATTATTGTGGAT GAACTTTCTAATCTAAAGAAGAATCGGAGAGTATACAAGCAGCAACAGAACAGCAACATATTCTTTCTTG AAGATCGAACAAAAATCTTTTCTGAAAGCAAAAATACATTAGACGAACTAAAAAAAGAATACCAAGCATT AGAAACCTCCGAGACAACCAAAGTCAAGACACAGTCAACCTGACTTCACATAACCATGTGTGGCATTTGC TGTTCTGTAAGCTTCTCTATTGAACACTTCAGTAAAGAGTTAAAAGAGGATTTGCTGCATAATCTTAGAC GGCGGGGCCCCAACAGCAGCAGGCAGTTGTTAAAATCTGCTGTTAACTATCAGTGTTTATTTTCTGGTCA TGTTCTTCATTTAAGAGGTGTTTTGACTATCCAACCTGTAGAAGATGAACATGGCAATGTGTTCTTATGG AATGGAGAAGTTTTTAATGGAGTAAAGGTTGAAGCAGAAGATAATGACACCCAGGTTATGTTCAATAGCC TTTCTGCCTGTAAGAATGAGTCTGAAATTTTGCTGCTCTTCTCTAAAGTGCAAGGTCCATGGTCGTTTAT CTATTATCAGGCCTCTAGCCATCACTTATGGTTTGGTAGGGACTTTTTTGGTCGGCGTAGCTTGCTTTGG CAGTTTAGTAATCTGGGCAAGAGTTTCTGCCTTTCGTCAGTTGGTACCCAGGTATATGGAGTTGCAGACC AGTGGCAAGAAGTTCCAGCATCTGGAATTTTCCAGATTGATCTCAATTCTGCTGCTGTTTCCAGATCTGT GATCTTAAAATTATATCCTTGGAGATACATTTCTAAGGAGGATATTGCCGAAGAATGTGGTAATGACCTG ACTCAGACTCCAGCAGGATTGCCAGAGTTTGTATCAGTGGTAATAAATGAAGCCAACCTGTACCTCTCAA AACCTGTCGTTCCCTTAAATAAGAAGCTGCCTGAGAGTCCATTGGAAATCCAATGTAGAAACAGTTCTAG CACTTCAGGTACAAGAGAGACACTTGAGGTATTTCTTACAGATGAACACACAAAAAAAATAGTTCAGCAG TTCATTGCCATCCTCAATGTTTCAGTCAAGAGACGCATCTTATGTTTAGCTAGGGAAGAAAACCTGGCAT CAAAGGAAGTTTTAAAAACTTGCAGTTCGAAAGCAAACATTGCGATCCTGTTTTCTGGAGGTGTTGATTC TATGGTGATTGCAGCCCTTGCTGATCGTCATATTCCTTTAGATGAGCCAATTGATCTTCTGAATGTGGCT TTTGTGCCTAAACAAAAAACAGGGCTACCTATTCCTAACATAGAAAGAAAACAGCAGAACCACCATGAGA TCCCTTCTGAAGAGTCCTCTCAGAGTCCTGCTGCAGATGAGGGGCCAGGTGAGGCTGAGGTACCAGACCG AGTCACAGGAAAAGCAGGACTAAAGGAACTACAGTCTGTCAACCCTTCTCGAACTTGGAATTTTGTGGAA ATAAATGTTTCTCTTGAAGAACTACAAAAACTAAGAAGAGCTCGAATATGTCACTTAGTTCAGCCATTGG ACACAGTTCTGGATGATAGCATTGGCTGTGCTGTGTGGTTTGCTTCTAGAGGAATCGGTTGGTTGGTGAC CCAAGATGCTGTGAGATCTTACAAGAGCAGTGCAAAGGTGATTCTTACTGGGATTGGTGCAGATGAGCAG TTGGCAGGTTATTCCCGTCATCGTGCCCGCTTTCAGTCTCTTGGCCTAGAAGGACTGAACGAGGAAATAG CAATGGAATTGGGTCGCATTTCTTCTAGAAACCTTGGTCGTGATGACAGAGTTATTGGTGATCATGGAAA GGAAGCAAGATTTCCTTTCCTGGATGAAAATGTTGTGTCTTTCCTAAATTCTCTGCCAGTTTGGGAAAAG GTAGACCTCACTCTGCCCCGTGGAGTTGGTGAGAAGCTTATTTTACGCCTTGCAGCTATGGAACTTGGTC TCCCAGCCTCTGCCCTTCTGCCAAAACGAGCCATACAATTTGGATCTAGAATTGCAAAACTGGAAAAATT TAATGAGAAGGCATCTGATAAGTGTGGAAGGCTCCAAATCCTACCTTAGAAAACCTCTCTCAAAATTACA TCATAATAAGATTATTCTATCCTGTACTTTGTGACAGTTACCTCAAAATAAACAGCTGCTGCCTTACTGT GAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3979452 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3979452 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19520 same embryo
	EMAGE:19519 same embryo
	EMAGE:19517 same embryo
	EMAGE:19518 same embryo
	EurExpress:euxassay_004908 same experiment
	MGI:4823279 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source