EMAGE mouse gene expression spatial database

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EMAGE:19527

Zfp74 zinc finger protein 74 ( MGI:107784)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004976. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004976_01	euxassay_004976_02	euxassay_004976_03	euxassay_004976_04

euxassay_004976_05	euxassay_004976_06	euxassay_004976_07	euxassay_004976_08	euxassay_004976_09

euxassay_004976_10	euxassay_004976_11	euxassay_004976_12	euxassay_004976_13	euxassay_004976_14

euxassay_004976_15	euxassay_004976_16	euxassay_004976_17	euxassay_004976_18	euxassay_004976_19

euxassay_004976_20	euxassay_004976_21	euxassay_004976_22	euxassay_004976_23	euxassay_004976_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19527_wholemount_strong.wlz

19527_wholemount_moderate.wlz

19527_wholemount_weak.wlz

19527_wholemount_possible.wlz

19527_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19527_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7983
Entity Detected:	Zfp74, zinc finger protein 74 ( MGI:107784)
Sequence:	sense strand is shown >T7983 AACCCAGCTCTCATTTTCAGAGTTCTTCCTGCGCTGAGGAGCCTTTTATAAACTCAATATTTATTGAATG TTGGACATATATCAGTCAATTGTTGGTGTGTGGGATATAGCAGCGAACAAAAGAAGCAGTGACCCCCCCC CCTCTCATGATGCTTACATTGCCGAGGACCAGATAATAAACAAATTCAGCTTGCTCAGGACCTCCCCCCT AAAAAGAAGAGGAAATGACCGAGTCTGAGGGACTAGTGACGTTCAAAGACGTGGCCATTGATTTTACCCA AGAAGAATGGAAGCAACTGGATCCTACTCAGCGGAACCTATATAGGAATGTGATGCTAGAAAACTACAAC AACTTAATCACAGTAGGCCCGCCACTCACCAAACCTGAAGTGATTTTCAAGTTGGAGCAAGAGGAAGAGC CTTGTGTGGTGGAGAGAGAAGTGCTGTGGAGACCCTGTCCAGGGGAGATTTTGGGAATTGACGAGCACCA GAAAATCCAGGACGGACAAGTTTTCGAGGGAATAGTGGTGACGAGTGAAGCCAGCGAATGTCCGGAGGAA TTTGCAAGTACGTTTTTCCCAAACGCAGACTCCATTCCTTCCATGCACAGTCTCTTTGAATGTGACGGTG TTGGAGAGTGTTTAGAGCCCAATTTTGGTGATGATGATGTACAATACCCGTTGCCAGAGGAACAGTTTGA ATATGACGATGCTATGCAGCCATTTCACACCAGCTCGCCCCATTTTGTGCTGACCCCCTTTAAGTGCAAC CATTGTGGAAAAGGCTTTAGTCAGACCCTGGACCTCATCAGACACCTGAGAGTTCACACAGGGGGGAAGC TCTATGAATGCCACCAGTGTGGGAAAGGCTTTAGCCACAAGGAGAAGCTGATCAACCATCATAAGCTCCA TAGCAGGGAGCAGTGTTACGAGTGTAGCGAGTGCGGGAAGACTTTCATCAAGATGTCAAATCTCATCAGA CATCAAAGGATCCACACTGGGGAGAAGCCGTATGTGTGCCAGGAATGTGGGAAATCCTTCGGCCAGAAGT CCAATCTCATTGATCACGAAAAAATTCACACTGGAGAGAAGCCTTACAAATGTAACGAATGTGGAAAATC CTTCAGCCAGAAACAAAGCCTTGTCGCACATCAGAAAGTTCACACTGGGGAGAAGCCTTACGCGTGCAAC GAGTGTGGGAAAGCCTTCCCTCGCGTGGCTTCCCTTGCTCTGCATATGAGGGGCCACACAAGGGAGAAGC CATACAAGTGTGATAAGTGTGGGAAAGCCTTCTCTCAGTTTTCCATGCTCATCATACACGTGAGGGTCCA CACCGGGGAGAAGCCTTATGAGTGTGGTGAGTGTGGCAAGGCCTTCTCTCAGAGCTCGGCACTGACAGTA CACATTAGAAGCCACACGGGCGAGAAACCCTACGAATGTAAGGAATGCAGAAAATCCTTCAGTCATAAGA AAAACTTCATCACCCACCAGAAAATCCATACTCGAGAGAAACCTTACGGATGCAATGAGTGTGGGAAAGC TTTTATCCAGATGTCAAACCTTGTCAGGCACCAGAGGATCCACACGGGAGAAAAACCTTATCTATGTAAG GAGTGTGGCAAAGCCTTCAGCCAGAAGTCAAATCTCATTGCTCATGAAAAAATTCACTCTGGAGAGAAAC CCTACGAGTGTAATGAGTGTGGGAAAGCCTTCAGCCAGAAGCAAAACTTCATCACCCATCAGAAAGTTCA TACGGGAGAGAAACCTTACGATTGTAATAAATGTGGGAAAGCCTTTTCCCAGATCGCTTCCCTCACGCTT CATCTGCGAAGTCACACGGGAGAAAAGCCTTACGAGTGTGAGAAGTGTGGGAAGGCCTTCTCCCAGTGCT CACTACTCAACTTACACATGAGAGGCCACACAGGGGAGAAGCCGTACGTGTGTAACGAGTGCGGAAAAGC CTTCTCTCAAAGAACGTCCCTCATCGTGCATATGAGAGGCCATACCGGCGAGAAGCCCTATGAATGTAAC AAGTGTGGGAAAGCTTTCTCCCAGAGTTCCTCCCTCACTATCCACATCCGGGGTCACACGGGCGAGAAGC CCTTCGACTGCAGCAACTGTGGGAAAGCCTTCTCTCAGATCTCTTCTCTTACGCTTCACATGAGGAAACA CACAGGCGAGAAGCCCTATGTCTGTATTGAGTGTGGAAAAGCTTTCAGCCAAAAGTCACACCTTGTCAGA CACCAGAGAATCCACACTCACTAGAGACGCCGTGACCACGGTGAATGTGAGGACTGCCTCCCATTGAAGT GAACACCTCATAGCACGCGACACAATCAGTTCTGGCGCTGACTGTAGGAGAGCCTTGGGAAGGTCCCATG CATCTGTTAGACAATTCCCACTTCGGGGACCAATTGTATGAGAAACTGTCACCAAACACCCAGTGATATC AGTATTCTCTTTCAAGTCTGAAAGAAAATATCTACTGTCAATCAACGAACTCGAGCTCCTTGCTGATGTA TTAAGCAAGAGAAGGAGATTGGAAAAACCAAAAACAATAACTTTTATAGGCAATATGAGGCTATGGGGAA GTAGGTTTTGAATGATAATGTATGTGTCGGGAAGAAAGTCTAAGGTAATTAGGTGACATGCGTACGAAGT TACAAAACGTGCTGACGAATGCAAAAGACCACTTGATAAAGCAAGTTAGAAAGTTATAAAAGTTCACGAA CCCTTCCCAGTCTCTCCCTTGTCCATACTTCCACTCAAAATTTACACATCAGTTATAAAGGATTTCATGA ACTGATTTCAGAGTAATACGGCAAGGGAAAGGACCACAAATAGCAAAAACAAAAAATACAGCAAAAAACA AAACAACAACAAAAAACAGTTTTTAACAAAACAACTAGGGAAGATGTTCACTCTCTACTAGATTATAATT ATCTAAAGTTTTTCTTATTTAAACTCTTTTGAGTGAATAAACTTTTGGAAACAAATTGAAACCCAAGACT TGGCAATTTTCTATCTGCCTGGTGCTACTGAAATCATTGGAATAAGTCTAGCCTGTTCAATATGTGTACA GGTTATGTGACCAAACTTGTTACTTCTTAAACAAAGATATACAGATTCCCTCAGGCTGGTGAGACGGCCC CACTGATAGGGGTTTGCTGCCAAGCTTCACAGCTGACTACGATACCCAGAATCCACATGGTGGGTGGAAA GAACCAACTTCTACACTTGTCCTCTGACCTCCAATGGCACGCCATGGTATGCATGTACACACACACACAC ACACACACACACACACACACACACTCAAAATAGGAAAGGTAAAATTCTAAAATGCTGGAAATAAAGTGAA ACATGTTGCTTTAGCGTAAGAATATTAAAAAAAATAATAAACCCTGCAAACATGGAATAGTCTATTTTCT TTTAGTGTACTGGACTCAGAAGTATCCATGTATGTCTGCAAAGATCTATCTATAAATGTTCCCAGATTTG TGGCTTGGTTTGCTCTAAGTGATATGTAACATTATCATTAATGCAGAGGTGGATCAATATATTTATTTCT ATGATAGGACACTATTCTTTATTTAAAAGATACAAAATTCACATGTAGTTACATGGCTGGATCATGATAT TTTGAGAGGAAACCCCAAAATATTCAATTAACCATGGTATAATATTTGTAAGTTAATAAAACAGTTCAAA GAACAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30011303 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30011303 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19531 same embryo
	EMAGE:19528 same embryo
	EMAGE:19529 same embryo
	EMAGE:19530 same embryo
	EurExpress:euxassay_004976 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source