EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19528

Zfp599 zinc finger protein 599 ( MGI:2679006)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004950. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004950_01	euxassay_004950_02	euxassay_004950_03	euxassay_004950_04

euxassay_004950_05	euxassay_004950_06	euxassay_004950_07	euxassay_004950_08	euxassay_004950_09

euxassay_004950_10	euxassay_004950_11	euxassay_004950_12	euxassay_004950_13	euxassay_004950_14

euxassay_004950_15	euxassay_004950_16	euxassay_004950_17	euxassay_004950_18	euxassay_004950_19

euxassay_004950_20	euxassay_004950_21	euxassay_004950_22	euxassay_004950_23	euxassay_004950_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19528_wholemount_strong.wlz

19528_wholemount_moderate.wlz

19528_wholemount_weak.wlz

19528_wholemount_possible.wlz

19528_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19528_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 12 13 14 15 weak expression: see section 11

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 09 16 17 18 19

pancreas

moderate

regional

moderate expression: see section 08 09 10 14 15

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 17 18 19 20 21 weak expression: see section 22

spinal cord

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 03 21 22 23 24

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10

rectum

moderate

regional

moderate expression: see section 12

midgut

moderate

regional

moderate expression: see section 12 13 14 15 16 17 19 20 21 22

lower jaw incisor

moderate

regional

moderate expression: see section 10 11 14 15

lower jaw molar

moderate

regional

moderate expression: see section 07 18 19

upper jaw incisor

moderate

regional

moderate expression: see section 14 15

upper jaw molar

moderate

regional

moderate expression: see section 07 18 19

metanephros

moderate

regional

moderate expression: see section 06 07 08 09 15 16 17 18

left lung

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12

right lung

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7986
Entity Detected:	Zfp599, zinc finger protein 599 ( MGI:2679006)
Sequence:	sense strand is shown >T7986 ACATGTTCTCCGTGTTGGATCCTGTCCAGGTGTTCCGCGCTCGCCCAGAAGTCCGGAGAGGTTCTGCCTG CCGCCGCGTGTCACATTTCCCCACTGAGAACCACAGTGAGAGCTCTGGGTGTCCCGGAATCCTGAGATGG GGCTGATATCATTTGAAGACGTAGCCGTTGACTTCACCTGGGAGGAATGGCAAGACCTGGACGCTGCTCA GAGGACCCTGTACAGGGACGTGATGCTGGAGACCTACATCAGCCTGGTGTCCCTAGGGCACTGTATGAAC AAACCTGAGTTAATCTTCAAGTTGGAGCAAGGACTCGGGCCATGGAGTGTCGCAGAAGCCTCAGACAGGA ACCTCTCAGATTTTCACATATTGACTGCACCAATTGTGACCAGCCAGAAAAATCACAAGGCATATATGTG GCAAGCTAGAACCACCGAAAATAAGGCATCAAATGAAAAGATTGCAGAGCTAAAGGAGCAACAGAAGATC CATCAAGGGTCAAAATCCTGCGAAAGAGAAGCACATGGGAAGACATTTTTTCAGAAAGCCCAGAGCACTG TGATTCAAATGTCTCCTACACGTCAGACAGCTCTCCATTACACGGCAACTCTCACTAAAGGTCAGAGACC TCACAGAGGGAAGATGAGCCGTGAGTATGAAGAATGCAGGAAAACCATCTTCCATAACTCACACGTTCCA GGATGTCAGAAAACTCTTATAGATACAAAGCTTTGTGGATGTACAGAGTGCAGAAAAGATTTCTCTTGCA ACTCAAAACTCACAAGTCATCCAAGAACTCGCATACGTAAGAGGCCCTATAAATGTAAGGAATGTGGGAA AGCCTTCTGCTCTCAGGGAAAACTTACTCTACATCAAATAGTTCACACAGGTGAGAAGCCCTATGAATGT ACAGAATGTGGGAAAGCCTTTTCCCACAAGGCATACCTCACTCAGCATCAGAAAATTCACATGAGCAAGA AGCCTTATGCATGTACTGAATGTGGGAAAGCTTTCTACCGATTATCACACCTTACTCTGCACCAGAGAAC TCACACAAATGAGAAACCTTATGACTGTACAGAATGCCAAAAATCTTCCTCCTGCCGGTCACAGCTTACT CTACATCAGCGAACTCACACAGGTGAGAGACCTTTTGAGTGCATGGAATGTGGAAAATCTTTCTACTACA AGGCACACCTAATTAGACATCAGAGAATTCACACCAACGAGAAGCCCTTTGAATGTATAGAGTGTGAGAA ATCCTTTTACTGCCAGTCGGACCTCACTGTGCATCAACGATCCCACACAGGTGAGAAGCCCTATGAATGT AAGGAGTGTGGGAAATCATTCTACCAGAAATCAAAACTCACTCTACATCAAAGAAATCATGTAGGTGAGA AGTCTTATGCATGCACAGACTGTGGGGAAGTTTTCTATTGCAAATCACATCTCACTCTACATCAGACTGT CCATACAGATGAACACCCATATATATGTACAGAGTGTGGGAAATGTTTCTACTATAAGTCACAACTCATT GTCCATGGAAGAACTCACACAGGTGATAGACCCTACAGATGCGGAGACTGTGGGAAAGCTTTCTCCCGAA AGTCACACCTCATTCGACATCAAAGTATTACTCACATAGATAAGAACAATTTGAATGTAGCAAATGTGGG AAAGGTTTCCACTGTAAGACCAGACTCACTTCACACTCACTCACTTTACCTCAGTGAACACAAGCATGTG CCAAGCATTTTGAAGGAAAAGAATGCAGGATAGCTTTGTACTTCAAGCCACAAATAACAGCAGAGGACAA CTCAGAGGAAGAGCCCTGTGAATGGGAAGAAAGAGAAAACTTTATATTGTAAGGCTGTGAGAAAGAACTC TGAAAATATGAGTTCAAGATACATAGTTTCCCAACTATGCAAAATACAAAGATGCAAAATGAATTATATG AGAGGCTTCACAAACCTAAAAGAACAGCAGCTGCGCTATGACCCAGGTTGAAAAAGATACAAAGGCAGGA AGACTCCTGAGTTCAAGGTCAGCCTGGAACAGAGCAAGGATAGGCCCAGGTGTGGTGGGAATTGTAATCT CAGGACTGGATCCCACCCAACTAAAGTTATTGTCTGTGTTTAACAAAGACAGATCTCTGAATTCATTTGC AATGAAGAAAGAAAGGAAAGAAACAAAGAAAGAAAGAAGATAAAGGTGTTTGCTGTCTCTTTCTAAGAAT CAAGGGGCTGGAGTCAGGGGATGCTGATTCATGGGATAATCAAAAGTGAACCTGGGGTAAATGATTGAAC TGATATGTAAATAAAAGATAGGTCGAAGAGATGAGTTATCCAGCATTTTCAAAATTGTATTCAAGCAAGA GAGAGAGCTGTCTTGAGCAGAACACAGGTTGGCTGTCGGCTTGTACCCATGATTGACTTTGAGTTTTTTT TACCGCTCTCAAACACCCCTTCTCTCAGGAACCCCTCTCCAAGCTGAGGCTGGTCCTTGGCACAGAAAAC CTGAATTGAATTCCCAGGTCTTCTGCTACCAGGAACTCTAGCTCCAGGTGATTCAACATCCTTGAATCTC CCTCTGCAGCTGAAGAAGGTTGCTGAGGCTAGGCATGTCCCTGTCCCTGCATGGAAGTCCAGAGAGGCTA TTGTATGAAATTGTGAAGGGGAGGCCTAGAGACTCCAAGAGGTTAGATATGCCAGACCTGTGGGATACCT GCCCAGGAAAGCTGCTACCAGGGAGTGCAACCAGCCCAAGAGAAAGAAGTTTGTTGCAGTCAACAAAGCT GAAAGGAGCTGGAGAGCTGAAGAACACTTTGATATCAAACATGGAGATGCAGAGTTGGGAGTTTGCCAGC TGGTTTTCAGTCCTGCTTTGGTCCAGTCTTTTCTCACTAGGCTCTCTTCCCTATGTTTTAGAATGGTGAT GTGTATCCTGTGCCGTGATACATTGAAAGTATGTGCTCTGCTTTTTATTTATTTATTTAATTTGAATTTT ATTTTATTTTATAGGGGATTACAATTAAGAGGTTGTACAAACCTCAGAAGAGACTTTGAACTTTGGACTT TAAAACATTGTTGAGACTGATAGACTGTGGGGATTTTTTGAAGTTGGACTAAATGCATTTTGCATTATAT TATGGTTACAAGCCTATGGGGGAAGGAAGTAGAATGTGGCAGTTTGAATAGCTATGGCCCCATTGTCAAT GGCCTGAAAACCTTTTGATAATCATGATCTCTATTGGGAAGTTAAGGCAGGAGAATGAATTCAAAGCCAT CCTCAGCCACATGGCATGTTCCAGGTTACATAAGACCCTGTTTCAGAAAGCAAAAATAGAGCTGGCTCAA TGGTTAAAAAAAACAGATGTCCTTCCAGAGGATCTGAGTTCTATTTCCAGCACCCACATGACAGCTAACA ACTGTCTATAACTCCAATTCTAAATCCAACACCTTTTGCTGGTCTCTGACAGCAATGTACACGTGTGGTC CACAGATATCCATGCTGGTAAAATACTCATAACACATGAAATAAAAATAAATAAATAAACAAATAATTAA AATAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30019042 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30019042 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19531 same embryo
	EMAGE:19529 same embryo
	EMAGE:19527 same embryo
	EMAGE:19530 same embryo
	EurExpress:euxassay_004950 same experiment
	MGI:4829324 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source