EMAGE mouse gene expression spatial database

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EMAGE:19573

Zfp263 zinc finger protein 263 ( MGI:1921370)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005032. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005032_01	euxassay_005032_02	euxassay_005032_03	euxassay_005032_04

euxassay_005032_05	euxassay_005032_06	euxassay_005032_07	euxassay_005032_08	euxassay_005032_09

euxassay_005032_10	euxassay_005032_11	euxassay_005032_12	euxassay_005032_13	euxassay_005032_14

euxassay_005032_15	euxassay_005032_16	euxassay_005032_17	euxassay_005032_18	euxassay_005032_19

euxassay_005032_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19573_wholemount_strong.wlz

19573_wholemount_moderate.wlz

19573_wholemount_weak.wlz

19573_wholemount_possible.wlz

19573_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19573_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7993
Entity Detected:	Zfp263, zinc finger protein 263 ( MGI:1921370)
Sequence:	sense strand is shown >T7993 GACAAGAGGTAATCCCCGTGGCCTACTGCTGTACAGAACCCAGGGCGTCTCTCAAGGCGGACGTAGCTGG TGTCGGGACCTAGGCTGGTGTCGGGGCATGACTATGGCTGCGGGCCCAAGCTCGCAGGAGCCAGAAGGGC TCCTCATAGTGAAACTGGAGGAGGACTGTGCCTGGAGCCACGAGGTACCCCCGCCTGAGCCAGAGCCCAG TCCGGAGGCCTCCCATCTGCGATTTCGACGGTTCCGCTTTCAGGACGCCCCGGGTCCCCGGGAGGCCCTT AGCCGGCTTCAGGAGCTTTGCCGCGGGTGGCTGCGGCCGGAGATGCGCACCAAGGAACAGATCCTGGAGC TGTTGGTGCTGGAGCAGTTCTTGACTATCCTTCCCCAGGAGATTCAGAGCAGGGTGCAGGAGCTGCGCCC AGAGAGCGGCGAAGAAGCAGTCACTCTTGTGGAGCGTATGCAGAAAGAACTTGGGAAACTGAGGCAACAG GTCACAAACCAAGGGCGGGGAGCAGAAGTGCTTTTGGAGGAGCCTTTGCCACTGGAAACAGCAGGAGAGT CACCGAGCTTCAAGCTGGAGCCAATGGAGACTGAGCGAAGCCCTGGCCCCAGGCTGCAGGAGCTGCTAGA CCCCAGCCCCCAAAGGGACTCCCAGGCTGTAAAGGAGAGGGCATTATCCGCTCCCTGGCTCTCTCTCTTC CCTCCTGAAGGGAATGTGGAAGATAAGGATATGACTGGGACCCAGTTGCCCGAGAGCCTGGAGGACATGG CAATGTATATCTCTCAGGAGTGGGACCATCAGGATCCTAGTAAGAGAGCTCTATCAAGGTATATGGTACA AGATAGCTATGAGAACTCGGGGACTCTAGAGTCCAGCATTCCCAGTCAGGAGGTCTCAAGCACCCACGTG GAGCAAGGAGAGAAGCTGTGGGATTCAAGTGTCCAGACCTGCAAGGAAGGCATGAACCCCAGAAACCCAG TTCCAGGAGTGGAAAAGTTTGAGAACCAAGAAAGAAATGTTGAGTCTGTTTCTCCTGAAAGTACCCATCC TCCAGTACTATTGCCTGGCCAGGCTAGAAGGGAGGTGCCCTGGAGTCCTGAGCAGGGAAGACTTGATGAC AGAGAAGGACATTGGGAGTGTCCCCCAGAGGACAAAATAGAGGAGTCTTTAGTGGGAACCCCAAGTTGTA AGGGACTGGTGCAAGCAAAAGAACAGCCCAAGAAACTCCATTTGTGTGCCTTGTGTGGCAAGAACTTTTC TAATAACTCAAACCTAATTAGGCACCAGAGAATACATGCAGCAGAAAAGCTGTGTATGGATGTAGAGTGT GGTGAAGTCTTTGGTGGACACCCACATTTTCTGTCACTGCACAGAACACACATAGGAGAGGAAGCTCATA AGTGCCTTGAATGTGGAAAATGCTTCAGTCAAAATACCCACCTCACCCGCCATCAGCGCACCCACACAGG GGAAAAGCCCTCCCAGTGCAATGCCTGTGGGAAAAGCTTCTCTTGCAACTCCAACCTCAACAGACACCAA AGGACACACACTGGGGAGAAGCCCTACAAGTGCCCAGAGTGTGGGGAGATCTTTGCTCACAGCTCCAACC TGCTTCGTCACCAGAGGATCCACACTGGAGAGAGGCCCTATCGCTGTAGTGAATGTGGCAAAAGTTTCTC TCGGAGTTCACATCTTGTTATTCATGAAAGAACTCATGAGAAAGAGAGACTCGACCCTTTCCCTGAGTGT GGTCAAGGCATGAATGACAGTGCCCCCTTTCTTACAAACCACAGAGTGGAGAAGAAACTCTTTGAATGTT CCACTTGTGGGAAAAGTTTCCGGCAGGGCATGCACCTTACCAGACATCAGAGAACACACACAGGAGAAAA ACCGTATAAGTGTATCCTTTGTGGGGAAAACTTCTCTCATAGATCTAACTTAATCAGGCACCAGAGGATT CATACAGGAGAAAAACCCTATACCTGTCACGAGTGTGGAGACAGTTTCTCTCACAGTTCCAACCGGATTC GTCACCTGAGAACCCATACAGGAGAAAGACCTTATAAATGTTCTGAATGTGGAGAAAGCTTCTCTCGGAG CTCACGTCTTACAAGTCATCAAAGGACTCACACTGGTTAGAAATTGTAGGATTTCTTTTTGCCTCAGTTT TGGCAGAAAACTGTCTTATCCAGAGCTGATATTCTTTGCCCACTTTGGTGTATTTTGCTGGTAATTAATA TAAAGAGAAAATGGGACTTATTTTAAGGGAGGTGCAGAGGTGAAAAACTAGCTTAAAACTAAAAAGTTCA GTCTTCAGAAGAGTTTAAGGTTCTTGAGGTAGCCTGCTCAGGGATGGTTTCCTATGAAATCCATCCTGTT TTCAGATGCATCAACCTTTATTTTGACCCAGGTATGGTTTGGATGCTGTATAAGTCTAGTATTAATCGCA TCAATTTTGAGGATACCTCTGGCTTCAACCCCTTATTTGTGACATTAAGCAGGAGAGTTTGGGCTCCTGA GGCCCTTGAGACCAAAGAAGGGCTAGGTCCTGGGACACCAATTTAAGGTCAACAAGCCCTTAAATTTAAG GTCAACAAGACCTTAGGTTGGGTTGGGGAGTAGGGGGTTGGGTTTTTTGTTGTTGTTGTTTGTTTGTTTT TTTGTTTTGTTTTAGGGCCCTGGCTGGGAAGTCCTGGGCAGTCCAGTTCTGCCACTGCAACACCCCTCTG AGTAGTTCGACAGGAGTGCTTATTGACATAAGAGGTGACCTCAGAAAAAAGGGAACTGGAGAGATAGACA GTTACAATGTGGAATCCACAGATGAACTCTGTGTTTTACAGATTAAGGGTATGTGATTACAGTCTACCTT TCTAAAGATTTGAGACTTTATTCCACCTGTCAAAAGGTACCTAGAGCTCTGTAGACATGATTATGAGTGG AGGGTTCCATGATTTCATCAGATACAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30009326 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30009326 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19576 same embryo
	EMAGE:19577 same embryo
	EMAGE:19575 same embryo
	EMAGE:19574 same embryo
	EMAGE:19578 same embryo
	EurExpress:euxassay_005032 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source