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EMAGE:19580

Rapgef3 Rap guanine nucleotide exchange factor (GEF) 3 ( MGI:2441741)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19580 EMAGE:19580 EMAGE:19580 EMAGE:19580 EMAGE:19580
"Pseudo-wholemount" of euxassay_005054. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005054_01 euxassay_005054_02 euxassay_005054_03 euxassay_005054_04
EMAGE:19580 EMAGE:19580 EMAGE:19580 EMAGE:19580 EMAGE:19580
euxassay_005054_05 euxassay_005054_06 euxassay_005054_07 euxassay_005054_08 euxassay_005054_09
EMAGE:19580 EMAGE:19580 EMAGE:19580 EMAGE:19580 EMAGE:19580
euxassay_005054_10 euxassay_005054_11 euxassay_005054_12 euxassay_005054_13 euxassay_005054_14
EMAGE:19580 EMAGE:19580 EMAGE:19580 EMAGE:19580 EMAGE:19580
euxassay_005054_15 euxassay_005054_16 euxassay_005054_17 euxassay_005054_18 euxassay_005054_19
EMAGE:19580 EMAGE:19580 EMAGE:19580 EMAGE:19580
euxassay_005054_20 euxassay_005054_21 euxassay_005054_22 euxassay_005054_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19580Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19580_wholemount_strong.wlz
19580_wholemount_moderate.wlz
19580_wholemount_weak.wlz
19580_wholemount_possible.wlz
19580_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19580_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
thalamus
weak weak
regionalweak expression: see section 07
thalamus mantle layer
moderate moderate
regionalmoderate expression: see section 08 09 11 12 13 weak expression: see section 07
telencephalon mantle layer
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7466
Entity Detected:Rapgef3, Rap guanine nucleotide exchange factor (GEF) 3 ( MGI:2441741)
Sequence:sense strand is shown

>T7466
CCCACGCGTCCGGTGCCAGCTGGCTCTTACATCTGGGACGGAGGCGGAGGCTTGGAGTTCTTAGCCTTTG
GGAAGGGGTGGGGGCTGCCCCACACCCCCAGCCCTCTATTCACCCAGGACAAACCCTATCTGCCTAGCCC
TGCATCTTGCTGCTCCAGGCAACCTGTCTGACTAGGGGCTGGCCCTTCGCTGCATCTCGGGACTCACACT
CATACCGCTCCAGGGATCCTGCAGCCTCTTGCTAGGGCGAGCACCGCGGTGTGAGCTGGAGAAGAAACAT
GAAGGTCAGCTGGCCAGGTGAGAACCACTGGCAGGTGGGCCCAGCTGTGGTGGAGAGTCCAGCTGTGGGG
GCACCGCAGGTGGGAGGTCTCCCGGACGTGGTTCCGGAGGGCACGCTGCTCAATATGGTGTTGAAGAGAA
TGCACCGTCCCCGCTGCTGCTCTTACCAGCTAGTGTTCGAGCACCGGCGCCCCAGCTGCATCCAGGGACT
CCGCTGGACACCACTTACCAACAGCGAGGACTCCCTGGATTTCAGAGTGAGTCTGGAGCAGGCCACCACA
GAGCATGTGCACAAGGCAGGGAAGCTCCTGCACCGCCATCTCTTGGCCACGTACCCTACCCTCATCCGAG
ACAGAAAATACCATCTGCGACTATATCGGCATTGCTGCTCTGGCCGGGAGCTAGTGGATGGGATCTTGGC
TCTGGGGCTTGGGGTCCACTCACGGAGCCAAGCCGTGGGCATCTGCCAAGTGTTGCTGGATGAGGGTGCC
CTTTGTCATGTAAAACATGACTGGACCTTCCAGGACCGAGATGCCCAATTCTACAGGTTCCCTGGACCGG
AGCCCGAGCCTACAGGAACTCAAGATGTGGAAGAGGAGCTTGTTGAGGCTATGGCCCTCCTGTCCCAGCG
AGGGCCTGATGCCCTACTCACCGTGGCGCTCCGGAAGCCCCCAGGTCAGCGTACGGATGAAGAACTGGAC
CTGATCTTTGAAGAGCTGCTGCATATCAAGGCGGTGGCACACCTTTCTAACTCGGTGAAGCGGGAACTAG
CTGCTGTTCTGCTCTTTGAACCACACAGCAAGGCAGGAACTGTGTTGTTCAGCCAGGGGGACAAGGGTAC
CTCGTGGTACATTATCTGGAAGGGATCTGTCAATGTGGTGACCCATGGCAAGGGGCTGGTGACCACGTTG
CACGAGGGAGATGACTTTGGACAGCTGGCTCTGGTGAACGACGCACCTCGGGCAGCCACCATCATCCTTC
GAGAAAATAACTGTCACTTTCTGCGTGTGGACAAGCAGGACTTCAACCGCATCATCAAGGATGTGGAAGC
AAAAACCATGAGACTGGAAGAACACGGCAAAGTGGTCTTAGTTCTGGAGAGAACCTCTCAGGGTGCTGGC
CCTTCCCGTCCCCCGACCCCAGGCAGGAACCGGTATACGGTCATGTCTGGCACCCCAGAGAAAATCCTAG
AACTGCTGTTGGAGGCTATGAGACCGGATTCCAGTGCTCATGACCCAACAGAGACGTTCCTCAGTGACTT
CCTGCTGACCCACAGTGTCTTCATGCCCAGCACCCAGCTCTTCACTGCCCTCCTGCACCACTTCCACGTG
GAGCCAGCAGACCCTGCTGGAGGCAGCGAGCAGGAGCACAGCACCTACATCTGCAACAAGAGGCAGCAGA
TCCTGCGGCTAGTTGGCCGATGGGTGGCCCTGTATAGCCCGATGCTCCACTCGGATCCCGTGGCCACCAG
CTTCCTCCAGAAACTCTCAGACCTGGTGAGCAGAGATGCCCGACTTAGCAACTTGCTGAGGGAACAGTAT
CCAGAGAGACGGCGACACCACAGGTTGGAAAATGGCTGTGGGAACGTATCTCCTCAGACCAAGGCCCGGA
ATGCACCTGTTTGGCTCCCTAACCAGGAGGAACCCCTCCCAAGCAGCGCGGGTGCCATCCGAGTTGGGGA
CAAAGTCCCCTACGACATCTGCAGACCTGACCACTCAGTGCTGACCCTGCACCTGCCGGTGACGGCCTCG
GTGAGAGAAGTGATGGCAGCTTTGGCCCATGAGGACCACTGGACCAAGGGACAGGTGTTGGTGAAGGTCA
ATTCTGCCGGTGATGTCGTTGGCTTGCAGCCAGATGCCCGTGGTGTGGCCACATCCCTGGGGCTCAACGA
GCGGCTCTTTGTTGTCGACCCACAGGAAGTGCATGAGCTGACCCCACACCCTGAGCAGCTGGGCCCCACT
CTGGGTTCTTCTGAGATGCTGGACCTAGTGAGCGCCAAGGACCTGGCAGGCCAGCTGACCGACCATGACT
GGAACCTCTTCAACAGGATCCACCAGGTGCAGGAGCACCTGCGGGATGTCACCACCGCGAACCTGGAGCG
CTTCATGCGCCGCTTCAACGAGCTGCAGTACTGGGTGGCCACAGAGCTCTGTCTCTGCCCTGTTCCTGGC
TCCCGGGCTCAGCTACTCAGGAAGTTCATCAAGCTGGCAGCCCACCTCAAGGAGCAGAAGAACCTCAACT
CTTTCTTTGCGGTCATGTTTGGCCTCAGCAACTCGGCCATCAGCCGGCTGGCCCACACCTGGGAGCGCCT
GCCCCATAAAGTACGGAAGCTGTACTCAGCCCTGGAAAGGTTGCTGGACCCTTCCTGGAACCACCGAGTG
TACCGATTGGCTCTCACCAAGCTCTCCCCTCCTGTCATCCCCTTCATGCCCCTGCTGCTCAAAGACGTGA
CCTTCATCCATGAGGGGAACCACACACTGGTAGAGAACCTCATCAACTTTGAGAAGATGCGGATGATGGC
CAGAGCGGTGCGGATGCTTCACCACTGCCGAAGTCACAGTACCGCGCCTCTATCACCACTCAGAAGCCGA
GTGTCCCACATCCACGAGGACAGCCAGGGATCAAGGATCTCCACGTGTTCTGAGCAGTCCCTGAGCACCC
GGAGTCCAGCCAGCACCTGGGCTTATGTCCAGCAGCTGAAGGTCATTGACAACCAGCGGGAACTGTCCCG
CCTCTCCCGGGAGCTGGAACCATGAGGAAGGACTGGCTGGAGCAGGCACTCTCTCCGAGGAAGCCAGAGC
CTGTGCAACCAAGAGGTCCAGAGGCCAGCCACAGCTGGGCAGGGCTCTCCACAGAGCGGACTCAAGGCCC
TGGAGTGGGCAGTGTCGGGGCAGTTGTCCCTGTGATGACTGTCAGCTATGGAGATCTTTGTTCACGGCCA
AGGAAAGGGGGGACATTGAGGCCATGCCGTGGGTAGGAGAGCTGGGAGGTACAGGACTCTGGATCAGTAG
AGAGCCCTCCCACCGGGCCTTCTGCGCATCTGCACGTCTGTACATCTGCATGTTTGCAAGTCTGTACGAC
TGTACGTGCAGCTGTACCATCCAGTGGTCGCCAGGCCTCGTGCTTGTTTTCACTGGGGCCAGTGAGCTCA
CATCTGCACAGTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGAGAGCCCTGGGTGGGGCCTTG
CCTGGGAGAGGGTGGAGAGTTCTGCTTTTCTCTCCACACATCTGAGATAAACAGCTGGGTGTAGGCAGGG
CTGTTGCCTAGAGCGGTGGTCTCAGAGGAGTTGTATTGTTTCCATAAATTAACAAAGATAAAAAAAAAAA
AAAA
Notes:The probe template was PCR amplified from IMAGE:4236136 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4236136 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19579 same embryo
 EMAGE:19582 same embryo
 EMAGE:19583 same embryo
 EMAGE:19581 same embryo
 EurExpress:euxassay_005054 same experiment
 MGI:4827612 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS