EMAGE mouse gene expression spatial database

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EMAGE:19598

Nars asparaginyl-tRNA synthetase ( MGI:1917473)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005019. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005019_01	euxassay_005019_02	euxassay_005019_03	euxassay_005019_04

euxassay_005019_05	euxassay_005019_06	euxassay_005019_07	euxassay_005019_08	euxassay_005019_09

euxassay_005019_10	euxassay_005019_11	euxassay_005019_12	euxassay_005019_13	euxassay_005019_14

euxassay_005019_15	euxassay_005019_16	euxassay_005019_17	euxassay_005019_18	euxassay_005019_19

euxassay_005019_20	euxassay_005019_21	euxassay_005019_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19598_wholemount_strong.wlz

19598_wholemount_moderate.wlz

19598_wholemount_weak.wlz

19598_wholemount_possible.wlz

19598_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19598_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8061
Entity Detected:	Nars, asparaginyl-tRNA synthetase ( MGI:1917473)
Sequence:	sense strand is shown >T8061 AAGAGGTAATCCCTTCAGTGCACGTTGGGGTGAGCAGGCGGCGGTGGTGCAGTGTCTATGTCCTCGGAGG TGATTAGGGGGACTGCAGAGATGGTTCTAGCAGAGCTGTATGTATCTGACCGAGAAGGAAATGACGCAAC CGGGGATGGAACCAAGGAGAAACCATTTAAAACAGGCCTAAAGGCTTTGATGACAGTTGGAAAAGAGCCA TTTCCCACCATTTATGTGGACTCACAAAAGGAAAATGAGAGATGGGATGTTATTTCTAAGTCACAGATGA AGAACATTAAAAAGATGTGGCACAGAGAACAGATGAAGAACGACTCTAGGGAGAAGAAAGAGGCAGAAGA TAACTTACGAAGAGAAAAGAACCTGGAGGAAGCAAAAAAAATTATTATTAAAAATGACCCGAGCCTGCCA GAGCCGGCCTGTGTAAAGATTAGTGCATTAGAAGGATACAGAGGCCAGAGAGTGAAGGTGTTTGGCTGGG TCCACAGGTTGCGCAGGCAAGGAAAGAATTTGATGTTTTTGGTGTTGCGAGATGGTACGGGTTATCTTCA GTGTGTCTTGTCAGATGACTTGTGTCAGTGTTACAATGGAGTAGTCCTGTCCACCGAGAGTAGTGTGGCG GTGTACGGAACACTAAACCTAACTCCAAAGGGCAAACAGGCTCCAGGAGGCCATGAGCTGAGCTGTGACT TCTGGGAACTGGTGGGGCTGGCCCCGGCTGGAGGAGCTGATAACCTGATCAACGAGGAGTCTGATGTGGA TGTCCAGCTCAACAACCGGCACATGATGATCCGGGGAGAGAACATGTCCAAAATCCTGAAAGCGCGCTCC ATGATCACCAGGTGTTTCCGGGACCACTTCTTCGACAGGGGCTACTGTGAAGTTACCACTCCAACACTGG TGCAGACGCAGGTGGAAGGTGGGGCCACGCTCTTCAAGCTTGACTATTTCGGGGAAGAAGCGTTTTTGAC CCAGTCCTCACAGTTGTACCTGGAGACCTGCCTTCCAGCCCTGGGAGATGTTTTTTGTATAGCCCAGTCA TACAGGGCCGAACAGTCCAGGACACGAAGGCACCTGGCTGAGTTCACTCACGTGGAAGCTGAGTGTCCTT TCCTGACCTTCGAGGACCTCCTGAACCGTTTAGAGGACCTAGTGTGTGATGTGGTGGACAGAGTCCTGAA GTCACCAGTGGCAAGCATAGTGTATGAGCTCAACCCGAACTTTAAACCCCCCAAGCGGCCTTTCCGCAGG ATGAACTACTCGGATGCTATTGAGTGGCTGAAGGAGCATGATGTCAAGAAAGAAGACGGGACTTTCTACG AGTTTGGAGATGATATTCCCGAAGCGCCTGAGAGACTGATGACAGACACCATTAATGAACCAATCCTGCT GTGTCGCTTTCCTGTGGAGATCAAGTCTTTCTATATGCAGCGCTGTCCTGAGGATCCTCGACTTACTGAA TCTGTGGACGTGTTGATGCCCAACGTTGGTGAGATTGTAGGTGGCTCGATGCGCTCCTGGGACAGTGAGG AGATTCTCGAAGGCTATAAGAGGGAAGGGATTGACCCCGCTCCTTACTACTGGTATACAGATCAGAGAAA ATATGGCACCTGTCCTCATGGAGAGTATGGCTTGGGCTTGGAACGATTTCTTAGCTGGATTCTGAACAGG TATCACATCCGAGATGTGTGCCTGTACCCTCGATTTCTCCAGCGCTGCAGGCCATAACCATGGGGCCCTC AAGCACGAAGGAAATGAAAAGTTAAGACTGCCTCTGCAAAAGAACAAACGCCCAAATCGTCCCTTGACTT CCTGTTGGGGGTAGTAGCTTTCTCTTGACTTTCCCATTATCAGAAAAAGCAGTAGGTATCACTTGAACAT CAAGTGACATTGATGCTCCTTTAATGAATATACTCATTACAAAATTTTGAGGAAATATATGTCATGTCAC TCTTTAGTATGGAGATATTTTATTGTGCTATAGGTTACAATCATTGTATTAAATAAACCATACATGATTA TACAGTTTTTAATTATGGTCTGACATCCAGTAAAATTCCATCTTTTTGTCCTTTTAAGTGTCGCTGGGAT TCTCGAATTCAAGCATCTAAAAGAGAAATTGGCTGTTTGACATAAAAGATACTGGAGTCAAATACATAGC CCTTTACATTCACTGTTAGTCTTTTCCTTATTCATATAGTGTTTATAATTCTGAGGGGTATGAGTTGACA GGGGAAATTAATGAAGGGGAAATGGATTATAATTTGCAATGAACATGATTGGATGGTGGGTTATTCAAAT GATTCCAACCCCGTTAGCCTTTAGTTTAAGAACGGGGCAGATGGGGCTTCCCTCTTTGTAATTCCTGAGT AACAAAAGTAAATCTGTTTGCAAAGACTTTGCACCTGAATCTTAGCCACCGTAGACAGCTACGTCATCCC TGCCTGGTCCTTGGGTGCCATCTGCACTGCCTTGCCGACTCAGCAGGGCTACCAGTGGCTGAAGTGCAGT TGCTCTCCCCAGATGCATGCCGTGTGTTCTAATGGGCTGTATCCCACTAACCAATAAATGAGCAGTGGTT AAAAAACAAAAAAAAAAAAAAAAAAG
Notes:	The probe template was PCR amplified from IMAGE:30057822 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30057822 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19602 same embryo
	EMAGE:19599 same embryo
	EMAGE:19601 same embryo
	EMAGE:19600 same embryo
	EurExpress:euxassay_005019 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source