EMAGE mouse gene expression spatial database

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EMAGE:19601

Tex19.1 testis expressed gene 19.1 ( MGI:1920929)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004973. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004973_01	euxassay_004973_02	euxassay_004973_03	euxassay_004973_04

euxassay_004973_05	euxassay_004973_06	euxassay_004973_07	euxassay_004973_08	euxassay_004973_09

euxassay_004973_10	euxassay_004973_11	euxassay_004973_12	euxassay_004973_13	euxassay_004973_14

euxassay_004973_15	euxassay_004973_16	euxassay_004973_17	euxassay_004973_18	euxassay_004973_19

euxassay_004973_20	euxassay_004973_21	euxassay_004973_22	euxassay_004973_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19601_wholemount_strong.wlz

19601_wholemount_moderate.wlz

19601_wholemount_weak.wlz

19601_wholemount_possible.wlz

19601_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19601_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

medulla oblongata

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

metencephalon

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

midbrain mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

spinal cord

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14

lower jaw incisor

strong

regional

strong expression: see section 11 15

lower jaw molar

strong

regional

strong expression: see section 07 18

upper jaw incisor

strong

regional

strong expression: see section 11 15

upper jaw molar

strong

regional

strong expression: see section 07

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8012
Entity Detected:	Tex19.1, testis expressed gene 19.1 ( MGI:1920929)
Sequence:	sense strand is shown >T8012 TGGCTTTGGGGTCCATTAGCTGGCACCTGGGCGCTCAGTCAGGGAGCTCCCCATATATTGGAGCAAGTGT GAAGCTGAAGAAGCTTCTGGAAGCTCAAGCCTTTCTACTTCTCCAGAGAGCCTCTGTGGTTCTGTTGACC TGACCTCCTCAGTCCATCGAGCAAGTCTGAATCTGTGTGTCCGCTTAGCTGCAGACAGACTTCTTGACCA TGTGTCCCCCAGTCAGCGTTCGCCATGGGGCCAGGGGCATGTCCTGCCTCTATGGGGCATGGCTGTACCA TCTTGTCCATGGGGAACAGACGAAGATCTGCTTTGCTTGCTTCAAGGCAGCTTTCCTATTGAATAAACTC TACCTGGAGATGGGAGACTGGCAAGAGGAAGAAGAGGAGGAAGAGGAAGAGGAAGATGCCGATCTCCTGG AATACTTGTCAGAGTCAGAGTCAGATTCTGAGCAGGAGCCAGGGCCTGAGCAGGATGCATGGCGGGGATT GGGGTCCCTTTATGTGCCACAGAGTGTCTCTGAAGGGTCTGGGGTCCTGCTGCCAACCCCTGTGTGGACA CAGGGCATACTATTCTCCATTTTTGTGCCCACTGAGCTCTTTCCTCAGGAAGCTGTACCCCTGGATCTGG GTCCTGAGGATGCTGAGTGGACCCAGGCCCTTCCCTGGAGACTTGATGGGCTTTTTCCCTGCTCGCACCA GCTCATCCCTCCTCTGACTTGGTGGGATATTTTTGATGTGATGCCATCTCCTGGGCAACCTGTGTTGTTG GAGTTGAGATGCCACTGGCCCTTGGACCAGACAGTAGCACAATCCTGGTTGCAAGACCAGAAGTTTGTCC TCCTGTTGGATGGCGTCCAATCTAGGTGCCACCTGCTGTCAATGCGTGTCCGCTGGGTCGTAAGGACTCA GGTCCAGCACTGGCAGGTGTTGCTGGACCCTGGTGAGATGTGGGTGGCCCATTTTCGGAAAGAAGTTGGG CAGCACGGCCTGTACCATCAGAGCCTGAATCCCTGGAGGCTGAGCATCCTGACAGCTTCAGAATTAGGGA TGGAGTTATTGCCTGCCACCTGCTACCTGTGGAATAAAGGCTTCTGGGTAGGTTCCTTCTTGCCCTGGCA CATTAACATGCCAGAGACCCGGAGCTGGGAGCCAGGAGAGAGGCTGTTTATCACAGATGCTACTATTTGT GGTACTGACTACCACCTTGCTCAGTCTTTCCTTGATTCCCACCCCACCCCCCACCCCCTCCTGACCCTTA CTCCCTGATGCACATTCCTGAGACACTAAAGCTCAGACAGTCCCCAGGGCCCTGGGGACTGTGAAGAGCA AGAGGTTGCCTGTTCTGAGCAGCTCAGGGGAACAGTTGGTCCAGGACTTGAGGGGGCATCTTGAACATCC TGGGAGCTTATGAACCTCAGAGGGAAGTCTGGCATGTTCGTGTCAGTGTTCAGTGTTTGGTAGGTGAGGC CTAGCTGTATGTTTAGTTATATGGAGTGTTGTGTGGTGGGTGTTATGGGGGCTCCGGTCACAGATCTACG TATGTATGGACTCTGAGGCACTAGTTGACCTTACTGTCATAGGGGTCATATGCTTACTGTCTTAGGGTCA AGATACCTGATTTTAGGGTTCACTGTTTTTGTTGTTGTTGTTTTACTTTGTTCGTTACTCGTGCTCCTTT TGAGGGCTTTTGTTGAATAAAGTTGGTGTTTTAAATTTTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30024269 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30024269 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19602 same embryo
	EMAGE:19599 same embryo
	EMAGE:19598 same embryo
	EMAGE:19600 same embryo
	EurExpress:euxassay_004973 same experiment
	MGI:4828667 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source