EMAGE mouse gene expression spatial database

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EMAGE:19644

Gtdc1 glycosyltransferase-like domain containing 1 ( MGI:2444269)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005120. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005120_01	euxassay_005120_02	euxassay_005120_03	euxassay_005120_04

euxassay_005120_05	euxassay_005120_06	euxassay_005120_07	euxassay_005120_08	euxassay_005120_09

euxassay_005120_10	euxassay_005120_11	euxassay_005120_12	euxassay_005120_13	euxassay_005120_14

euxassay_005120_15	euxassay_005120_16	euxassay_005120_17	euxassay_005120_18	euxassay_005120_19

euxassay_005120_20	euxassay_005120_21	euxassay_005120_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19644_wholemount_strong.wlz

19644_wholemount_moderate.wlz

19644_wholemount_weak.wlz

19644_wholemount_possible.wlz

19644_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19644_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

telencephalon marginal layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

spinal cord

moderate

regional

moderate expression: see section 08 09 10 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8588
Entity Detected:	Gtdc1, glycosyltransferase-like domain containing 1 ( MGI:2444269)
Sequence:	sense strand is shown >T8588 GAAAAAGATCTTATACTTCCATGAAAACCAGTTGGTGTATCCTGTCAAGAAATATCAGGAGAGAGATTTT CAATATGGATACAACCAAATTCTCTCGTGTTTGGTGGCTGATGTTGTGGTATTCAATTCCAGTTTTAATA TGGAATCATTTCTTACTTCTATTGGAAAATTCCTGAAGCTGATTCCTGATCACAGGCCTAAGGATCTGGA AAGCATTATCAGACCCAAGTGTCAAGTTATTTATTTTCCCATCAGATTTCCTGATGTGAGCAGATTCATG CCCAAGCACAAAATAGCCCACTTAAGGAGGATGCTCAGCCTTATTGGAGATGCTGCTGCATCTCAGTCTG TGGCGCCTTGTCCCCAGCCAGGGCAGAGGGTTTCTGAGAAGTCACCGGAGAACTGTGAATCAAAGTCTGA TGAACACCCTGACCTTGATGCTGAACAAGAAGCCCTGGACAACCCATCAGTCCATAAATCAGGCTCTCTT CCTGTGAGTAAAGAAAACCTGCCTCTTGATCCCAGCACCCTTCTGTGTGGAGCTGAAGATCCGCAAAGAC CGCTGCACATTACATGGCCGCACAGGTGGGAGCATGACAAAGATCCAGAGACGTTCTTAAAGATACTAAT GAGTCTAAAGCAGTTAAACTTGAACTTCCATGTCTCTGTCCTCGGAGAGACTTTTACAGACACACCAGAT ATATTTTCAGAAGCCAAAAAGGCACTGGGCTCTTCAGTCTTGCACTGGGGCTACTTACCCCGTAAAGAAG ACTATTTCCGCGTACTGTGCATGGCTGACGTTGTCATCTCAACAGCCAAGCATGAATTCTTTGGAGTAGC AATGTTGGAAGCTGTGTACTGTGGCTGCTACCCACTCTGTCCCAAAGCCTTGGTCTATCCTGAGATCTTT CCAGCTGAGTATCTGTATTCCACACCTGAACAGCTTTCAAAACGGCTCAAGAGTTTCTGCAAGAGGCCAG ATATTATAAGAAAACATCTTTATAAGGGTGAAGTGGCTCCATTTTCTTGGGCAGCCCTACATGGTAAATT CAGGTCTCTGCTCACAACAGAACCCAGGGAAGATTTGTGACAGATGGGGCTAAGTCGCAAACTTGCAGCC TAAGGCAGAATCTGAAGAACTTTCCAGAGTGTGCCCATATTTACCTGATCAGAGAGAAAAGAAAATCTGC AGAGGAAGCCGAGCCTGGCTGCTTGTCATAGCTGACACAGAGCCATCTGCCACAAACCTGTGGCGGCTTC AGATCTCCAATCCCTGCCACCACCCCAACTCAAATTAAATACAGATTCCTAGAGACGTTATGATGGTTAC ACATGTCCTCGGCATCACATGTAGGAGACTGTTCAAAAAAAAATCTGTGGCCTGTTGTATAACCGCACTC ATGTATCCCATATGTGGTGCCACATTGAATTTCCGGTTGAATCCGTTTTTATCCTTTGTACTGGATGACA CGGAGCCTGAATTCTTTCTTTCTGCCGACACGATGGCAGCCAAACTGCAGCTTCAAACACTCACACTTGG CTGAGTTTCTACCTGGGTTGCCAGGTTATCATCGGAGCCTTCTTGTGTCCTCAAAGGGCCACGGGGCCTA GAAAGAGGATCAGAATGCTACTGAACTAATTGGGCAGCCATCTCAAAGCTGCTGTAGGCAAAGGGCTGCT GTAGCACTTTTCTTTTAGCAAATTAATGACTCTCTGGCAAAGGAGTGTTTAAGTGAAGGCATTAATAAGG GCTCTGGCAGGTATTCCCATGATTCTCAGAGTTCCATTTGCATTGAATTAATCTTAAAGAAACTTGCAAG ATAAACAGCTGGAATTCGGACAAACATGGCAGATTCAGTGAGTGAAGTCCTCTCATCCATAAAATGAACA CGAGATACTTGTTTTAAAATTTTTTCCCTGTGGGTAAAAATAGAAAAATCAGAATGCTTATAACAAAACA TAATTTGGTACAAAATTCTTCAAAATACTTGTATCCAACTATAAATATATTCTTCTTGAGATAAGTCACT TACGATTCTTGCTCTGTTTGTTGGTGTAAAGATGTTTTAAATGGCTGGATTGTAAAATACATTTTTAATA AACTGCCCGCTGCAATTTTAATTAGCATAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5715781 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5715781 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19647 same embryo
	EMAGE:19642 same embryo
	EMAGE:19645 same embryo
	EMAGE:19643 same embryo
	EMAGE:19646 same embryo
	EurExpress:euxassay_005120 same experiment
	MGI:4825270 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source