EMAGE mouse gene expression spatial database

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EMAGE:19647

Zfp341 zinc finger protein 341 ( MGI:2682937)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005136. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005136_01	euxassay_005136_02	euxassay_005136_03	euxassay_005136_04

euxassay_005136_05	euxassay_005136_06	euxassay_005136_07	euxassay_005136_08	euxassay_005136_09

euxassay_005136_10	euxassay_005136_11	euxassay_005136_12	euxassay_005136_13	euxassay_005136_14

euxassay_005136_15	euxassay_005136_16	euxassay_005136_17	euxassay_005136_18	euxassay_005136_19

euxassay_005136_20	euxassay_005136_21	euxassay_005136_22	euxassay_005136_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19647_wholemount_strong.wlz

19647_wholemount_moderate.wlz

19647_wholemount_weak.wlz

19647_wholemount_possible.wlz

19647_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19647_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8586
Entity Detected:	Zfp341, zinc finger protein 341 ( MGI:2682937)
Sequence:	sense strand is shown >T8586 CAAGATGGCGCAGGCGATCTTCGAGGCCCTGGAGGGAATGGACAATCAAACGGTTCTGGCCGTCCAGTCC TTACTGGATGGCCAAGGAGCAGTCCCTGACCCCACAGGACAAAGTGTAAATGCGCCACCTGCCATCCAGC CACTGGATGACGAGGATGTCTTTCTCTGCGGGAAGTGTAAAAAGCAGTTCAACTCACTGCCAGCCTTCAT GACCCACAAGCGTGAACAGTGCCAGGCAAACGCCCCTGCCCTGGCCACAGTCTCGCTGGCTACCAACAGC ATCTATACACCGTCAGCAGCACCCGCTGCCGTCCAGCAGGCCCCACCTCCTGCCAACCGCCAGATCTCCA CGTACATCACAGTGCCCCCCTCCCCACTGATCCAGACCCTGGTGCAAGGGAACATCTTGGTGAGCGATGA TGTGCTTATGTCTGCCATGTCTGCCTTCACATCCCTGGACCAGCCAATGCCTCCAGGGCCCCCACCTGTG CAGAACAGCTTGAACATGCATCCTGTGCCCAATTACCTCACCCAGCCTCCACCCCCTCCTCCACCCCCTC CGCCTCTCCCCCCACCCCCCCAGCCTCCTCCTCCCCCACCCCAGACTCTGGGCCCTTCTGGGCGCTCCAA CCCTGTTGGGAACAGTGTGGTGGAGGTGTACAATGCTGCTGCTCCCATGGCTGGGAACGGAACGGTGGAG ATCCAGGCGCTGGGGATGCAGCCGTACCCGCCCCTGGAGGTACCAAGCCAGTGTGTGGAGGCTCCAGTGT ACCCCACTCCCCCAGTGTACAGCCCTGGCAAGCAGGAATTCAAACCCAAAGGACCCAGTTCCACTGCCCC CATGACCAATGCAACTGGGAGCACGGTGGCCACCTTTGACTCCCCACCAACGTTGAAGACCAGACGTGCT AAAGGTGCCAGTGGACTCCCAGAAGCTGCAGGGAAGCCAAAAGCTCAGAAACTCAAGTGCTCCTACTGTG ACAAGTCCTTCACCAAGAACTTTGATCTTCAGCAGCATATCCGAAGCCACACAGGGGAGAAGCCCTTCCA GTGCATTGCGTGTGGCCGTGCCTTTGCCCAGAAGTCTAACGTCAAGAAGCACATGCAGACTCATAAGGTG TGGCCTCCAGGGCGAAGTGGCGGCACAGTCTCCCGAAACTCTGTGACCGTACAAGTCATGGCTTTGAACC CCAACAGGCAGGAAGAAGAAGACACGGGGTTGGGTCAGTCACTGTCCAGTACCACACAGCCCCAGGCCTT GCCCACGGCAGGTGAGGATGAAGGTGACAAGCCAGAGGCCAAGCAGGTGGTCCTGATCGACAGCTCTTAC CTGTGTCAGTTCTGCCCCAGCAAGTTCAGCACCTACTTCCAGCTCAAGTCCCACATGATTCAGCACAAGA AGGAGCAGGTGTACAAGTGCGTGGTCAAAAGCTGTGCCCAGATGTTCCCCAAGCTGGACACCTTTCTGGA GCACATCAGAAGCCACCAGGAGGAGCTGAGCTATCGCTGCCATCTCTGTAGCAAGGACTTCCCCTCACTG TATGACCTGGGCGTACACCAGTACTCCCACAGCCTCCTGCCGCAGCACAGCCCCAAGAAGGACAGCACCG TCTACAAGTGTGTCAAGTGTGTCAACAAATACTCCACTCCCGAGGCCTTGGAGCACCACGTGCAGACGGC CACTCATAGCTTCCCCTGCCCGCACTGCCAGAAGGTCTTCCCCTGTGAACGCTACCTGCGGCGTCACTTA CCCACCCATGGCAGTGGTGGCCGATTCAGGTGCCAGATCTGTAAGAAGTTCTTCCGGAAGGAGCACTACC TCAAGCTACACGCCCACATCCACTCAGGTGAGAAGCCCTACAAATGCTCAGTGTGTGAGTCTGCCTTCAA CCGCAAAGACAAGCTGAAGAGACACATGCTGATCCACGAGCCCTTCAAGAAATACAAATGCCCCTTCTCG ATGCACACGGGCTGCAGCAAGGAGTTCAACCGGCAGGACAAGCTGAAAGCCCATATCCTCTCCCACGCTG GCCTGAAGCTCCACAAGTGTGGGCTGTGCAGCAAGTCCTTCAGCCGTCGAGCTCACCTGGCAGAGCACCA ACGAGCCCACACAGGCAACTACAAGTTCCGCTGCGCGGGCTGCGCCAAGGGCTTCTCACGACACAAATAC CTCAAGGATCACCGCTGCCGCCTGGGCCCCACCAAGGACAAGGACCTGCAAGCCCGGCGGCCTCCCCGGA GGAGGGCAACGGCGCGTGGTGGTGGCAGCATCGCCAGCGGGCACAAGGAGGAGAACCCCATGCCAGACCC GCTGGGGCTGGAGGAGCTGAAGGCCGCAGGACCCTCGCCTGAGGCTGCCCCTGGCAAGCCACCCTTTGAG CAGGACGCTGTGTTGTCCATTGTGGTGGGCGGGGACCCGGAGCTGGTGGTGCCTGGGCACACAGAGGGTC TGGGCTCCAACCTGGCACTTGCCGAGCTGCAGGCTGGAACCGAGGGAGGGTGTGCCATGCTCGCCGTCCC AGTCTATATCCAAGCCTCAGAGTAACCAGGCTAGACTTCCCGGAGGTGTGTGTGTTGGGGGCGGGGAGGG TGCACTAGCACAATCCCTTTTGGAAGTTACTGTATCAGCCTTGCCCCACCGCTGTTCTTCCATGCCCCGA GATTATCTGGGGAAACCTGTAGCACTCACAGGGTCTGAGGCAGGCACCATAGCTCCAGGGCATAGCAGCG GTAAGAGAGATGGCCAGAGCATGAAGTGCTCAGGGCCTAGCTGATGGAGGCTGGTACTTGGGCATCCTTT AAGACACGGTAGGGTGGCCCCACCCACCTATCCAGGTTACCTGGGGTGGCCTCAGGTTGTGCTGCTCTTC AGGATGCTAGCTGGATGTGGCTGGAGTCCTTGTGTGTTAAACACTTCTTGATGAGGGATTCCTCAGACGA CTGTATGAGGTGAGCATGTAAGAGGCACTTATCCTGGGGCGTAGACTATAGCTGCCAGCCAGCCCTTGCT TCAGATTCACCACTGTGACCTGAGCAAGCACATCACCTCTCTCTGTTCCACGTTCCTCACCTGTGCAACA GGACTTCGTATAGCACTACCTCCTAGAGTGTTCCGAGGACATAGTATACGAAAATGTGCAAAGGACTGGG CACAGTACCCATGGCCTGGCAAGTGCTCAATAAATCTCAATAAATGTTTTTACCAACCTCATGGGTCCAA ACCAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5715767 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5715767 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19644 same embryo
	EMAGE:19642 same embryo
	EMAGE:19645 same embryo
	EMAGE:19643 same embryo
	EMAGE:19646 same embryo
	EurExpress:euxassay_005136 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source