EMAGE mouse gene expression spatial database

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EMAGE:19653

Mllt1 myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 1 ( MGI:1927238)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005124. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005124_01	euxassay_005124_02	euxassay_005124_03	euxassay_005124_04

euxassay_005124_05	euxassay_005124_06	euxassay_005124_07	euxassay_005124_08	euxassay_005124_09

euxassay_005124_10	euxassay_005124_11	euxassay_005124_12	euxassay_005124_13	euxassay_005124_14

euxassay_005124_15	euxassay_005124_16	euxassay_005124_17	euxassay_005124_18	euxassay_005124_19

euxassay_005124_20	euxassay_005124_21	euxassay_005124_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19653_wholemount_strong.wlz

19653_wholemount_moderate.wlz

19653_wholemount_weak.wlz

19653_wholemount_possible.wlz

19653_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19653_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8589
Entity Detected:	Mllt1, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 1 ( MGI:1927238)
Sequence:	sense strand is shown >T8589 CCCGGAACGGCGGCGGCGGCGGTGCGGCCCCCGACAGACAATGAGAGCGGCGGGGCGTTGCTGAGCGGCG GCAGCGAGAGCGACGCGGGCCCGGGGGCGGGGCGGGCGCCAGTCATGGACAATCAGTGCACTGTCCAGGT GAAGTTAGAGCTGGGGCACCGTGCCCAGCTACGGAAGAAGCCCACCACCGAGGGCTTCACCCATGACTGG ATGGTCTTCGTGCGTGGCCCTGAGCAGTGTGACATCCAGCACTTTGTGGAGAAGGTCATCTTCCGGCTGC ATGACAGCTTCCCTAAACCCAAGCGTGTGTGCAAGGAGCCCCCCTACAAAGTGGAGGAGTCGGGCTATGC AGGCTTCATCATGCTCATTGAAGTGTACTTCAAAAACAAGGAGGAGCCGAGGAAGGTCTGCTTCACCTAC GACCTGTTTCTCAACCTGGAGGGCAACCCTCCTGTCAACCACCTGCGCTGTGAGAAGCTCACCTTCAATA ACCCCACCACCGAGTTTCGCTGCAAGCTGCTCATGGCTGGAGGGGTGATGGTAATGCCCGAAGGAGCGGA CACGGTGTCCAGGCCCAGCCCTGACTACCCCATGTTACCCACAATTCCACTCTCTGCCTTCTCTGACCCC AAGAAGAACAAACCATCCCACGGCTCCAAGGATGCCAACAAGGAGAGTGGCAAGGCTTCCAAACCACACA AGGTGGCCAGGGAGCACCGTGAGCGGCCGCGCAAGGACTCAGAGAGCAGGAGTTGCTCCAAGGAGCCGGA GCGTGAGCCCAAGAGTGCCAAGGATGCTGCACGCAAGCTGCCCAAGGAGGAGAAGGCCCCAGTGCCCAAG GCTGCCTTCAAGGAGCCCAAGATGGCCCTAAAGGAAACCAAGCTGGAGAGCCTGTCCCCCAAGGGGGTGC CCCAGCCTCCTACCCTGCCCAAGGCCTCCAGCAAGAGGCCAGCTGCCACGGACTCACCGAAGCTCAGCGC CAAAAAGCCTAAAAAGAGTGGGTCAAAGGGGACACGGCGTGCACCAGGCACCTCACCCCGAGCTTCCTCC TTTCCTGACAAGAAGGTGGCCAGGGACAAGAACAGCAAAGGTGACAAGGGGAAGGCAGAGAGTGAGCCTC GGGAGGCCAAGCGGACTCTGCAGACTGAAGACTCAAACTCAGAGGATGAGGCCTCCTTCCGGTCAGAGTC GGCCCAGTCAAGCCCAGCTAATGCCAGCTCCAGCTCTGACTCCAGCTCTGACTCAGACTTTGAGCCGTCT CAGAACCACAGCCAAGGACCCCTGCGCTCCATGGTGGAGGACCTGCAGTCTGAGGGCTCTGATGAGGATG ACTCATCGTCAGGAGAGGAGAACACCGGCAAGGCCAACCCAGGCCGGGAGTCCAGGCTAAGCTTCAGTGA CAGCGAGAGTGACAACAGCGCAGACTCCTGCCTGCCTGGCCGTGAGCCCCTGCCTCCCCAGAAGCCGCCC CCTCCCAGCAGCAAGGTGTCTGGTCGGAGGAGCCCCGAGCCCTGCAGCAAGCCCGAAAAGATGCTGAAGA AGGCTACCTATGACAAGGCTTACACGGATGAGCTGGTGGAGCTGCACCGGAGACTGATGGCTCTGCGCGA GCGCAATGTGCTGCAGCAGATTGTCAATCTGATCGAGGAGACTGGCCACTTCAATGTCACCAACACCACC TTCGACTTTGACCTTTTCTCCTTGGATGAAAGCACTGTTCGCAAGCTGCAGAGCTGCCTGGAGGCCGTGG CCACATGACCCCGGCTGCCACTGTGGAGCCACGGTGGGCATGGTGGGCACGGGAGGGGCTGGGCATGAGG GCTGGCCCGCCATGGCTTCCGCTCCCGGTACCACCCCCAACTAACCATGGTGCCCCAGTACCCCTCAGTG GTTGGTGATGAAGGGGTGCTGCAGGCCACACACAGGGTGGGGTGACATAGGACACACGTGGCCTGCAGCC CCTCACTTAGTCCCTGCCAGTTGTCCATTGTGGGTGGGACTGAATGGCAGATTACTGCCTGGGCCTCTCT GGGTGGCCAGGATGACCACAGTCTTCCACACAGTTCACAGGCCTCTCTCAGCTGGCCTGCCTCCTAGACT TGAGGGTCCGTTAGCCTGGCCAGAGCCAGCTATGTGCACCTGCTTCTGCCATGCAGCCTTGTGCATGTGT GTGCTCCAGGTGCAGCCAGCAGCCGGGGGGCTCAGGCCACCCCTCTGCTGGAGGCACAGAGGATCTCCCC TCTAGATCCTAGGATGCCACTGCCTCTCGAGGCCCCAGGCCAGAGAACACTACAATGGGCACCAATCTTG GGACCCCAAGGGCCACCACTGCAGCCATGTGCCAGCTAAGCACCCACAGTTCCAAGGCTAGCTGGACTGG CAGCACTCCTGGGCCACCATTCCCCGTGGGCCTCCTCCTCTGCTCTCTCACTGATCAAGTCTTTGAACTT TGAAAACATGAGCTTTTGCCAAATATGATAGGATGGTGTGTGTGAGGCCGGCCCAACCCTAGACTGCCTC TGTTCCTGGCCACATGTGCGCCTGGAGAGGCTTTGGACCTGTGCCCTGCAAACTGCCAGGGGTCAAGCCA GCCCCCTTCCCCTGTCTGAGCCTGGAGTGCCACCCCTTCCCCAACTTCAGTTGTCGGTGTTTGTGCCCCA ACCAAGGTGTTTGAAAGAGGCCTGAGCTGAAGCCAGCTCACAGTTGCCTCGATGTGTTGCTTCCTCCCCA TTGTGGGTTCACCCCTGGAGGCCTGGCCTCCCAGCTCCCTCTCCCTGTCTGTCCCACTCCCAAACTATCT GTCAGGAAGATCCTGAACACTCCTGGGGACCCCAGCCTCTAAGGCTTCCTTTACCCCCAGAGAAAGGAAA AGGCCTCTGCCTGTAGCCCCTGTTCACACTCCCTATAGCTGGGAGGAAACAGGGCTCGTGCCCCAACCTC TTTCCACACAGGCCGACAATACTCTCCATCAGGTGTTCTGCAGCCTCTAAGCCTTTTCATAGAAGGCCCT GGCATCGTGGCAGCTGTTGGCCTCCACATGGAGTGCGCCAGGGTCCCAAACAGCCTCAGCCCAGGGCACC ATTCTTCCTCTCATGGTCGCTAGCGGCCCAGCCCTTCTGCTGCCCTGGGCCTTGGTGTCTAGTCCTTCCA GATGCTTCCAGGGTCGTCCTGAAAACACCCACCCATCTCTTCAGTTACCTCCTCTGGCCAGAAGGTCACA CCACTGTCCTCCAGAGCCACTCTGGGGCCCATACTGGCTGTCTGGAAGCCTAAAGCAGCAGCCTGCAGCC AGAGCCGAGGGTTCTAGGGACTTGTCCATAGGAAGGGGTGGCACTATGTATACCCTCAGTGAGTCCCTTT GCTGCAGCCTCCTGAACCAGTTCATGACACTGAGTGCAAAAGGTATTGGATACCTATCCCTCCCCAGGGC TTCACAAGACATGTCTCCATGAATAGACACGTCACTGGCCCAGACGAGCCCTGGACAGAGGGACCAGGAG AGCTGTGTTTGTAACAAGCTGCAGGGCCATTTGTCCCGCATTTTAAACTTTTTTGTTAAATTGTGAAATG ATGGAGGAGTTTTATAGAAAATAAAGTGAGATGGGAGCAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5716396 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5716396 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19651 same embryo
	EMAGE:19649 same embryo
	EMAGE:19652 same embryo
	EMAGE:19648 same embryo
	EMAGE:19650 same embryo
	EurExpress:euxassay_005124 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source