EMAGE mouse gene expression spatial database

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EMAGE:19673

Golga3 golgi autoantigen, golgin subfamily a, 3 ( MGI:96958)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006124_01	euxassay_006124_02	euxassay_006124_03	euxassay_006124_04	euxassay_006124_05

euxassay_006124_06	euxassay_006124_07	euxassay_006124_08	euxassay_006124_09	euxassay_006124_10

euxassay_006124_11	euxassay_006124_12	euxassay_006124_13	euxassay_006124_14	euxassay_006124_15

euxassay_006124_16	euxassay_006124_17	euxassay_006124_18	euxassay_006124_19	euxassay_006124_20

euxassay_006124_21	euxassay_006124_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19673_wholemount_strong.wlz

19673_wholemount_moderate.wlz

19673_wholemount_weak.wlz

19673_wholemount_possible.wlz

19673_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19673_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8550
Entity Detected:	Golga3, golgi autoantigen, golgin subfamily a, 3 ( MGI:96958)
Sequence:	sense strand is shown >T8550 AAAATTTTAAGTCCACTTTCTGAAGGATATCTCAATCTGTCCGGAGATCCATACCAACCCCAATTTGAAA GTGGCTACTGGCCGCTGAGGGCAGCCTCTCTTCCGGAACGAAGACCTCGCGGACCGTTTGTTTCCGGGGG GTTGCCCGAGACTGGTACCCTGTTGCGCCCCGCCCCTTGCACAGCCACGTCCTACCGGAGCCGCAGGGTT GGGCCGGAACCGGAATCATGGTTGCTTGGTTTGACTTCAAGCCATTTATGAGACATTAAACCTGAAAATG GAAAACAGAGTAAGATCCTCCTTAGAAGCTCTGAGCTGTTGCTAATGGCAAAAAACAAACTTAATCCAGA GTTACTTGTAGCTCCTCAGAGTCTTCTTAGTCTGGGCTGCCCTGACCATGGATGGAGCATCAGCCAAACA GGATGGCCTCTGGGAAAGCAAGTCCAGCAGTGATGTTTCATCTTGCCCTGAAGCCTCGCTGGAGATTGTG GGCTCTCTGGCCCGACTGCCTGATCAACAGGATACAGCTCAGGATGCCAGTGTTGAGGTAAACAGAGGTT TTAAGGAAGAAGGAAGCCCAGATAGATCCAGCCAGGTGGCCATCTGTCAGAATGGGCAGATCCCAGACCT TCAGTTGTCCCTTGATCCCACAACAAGCCCAGTGGGACCTGATGCCTCTACAGGCTCCACTGCATCTTCA TTGCCTTTGGAAAAGGAGGAGCAGGTTCGACTGCAGGCCCGGAAGCGCCTGGAAGAGCAGCTGATGCAGT ACCGAGTTAAGCGCCATCGGGAAAGGTCCAGTCAACCTGCAACTAAAATGAAACTTTTTAGTACACTTGA TCCTGAGCTCATGTTAAATCCAGAAAATTTGCCAAGGGCCAGTACTGTAGCTGTGACAAAAGAATATTCC TTCCTGCGTACCAGTGTTCCTCGAGGGCCCAAGGTAGGCAGCTTAGGGCTGCTGGCACATTCTAAAGAGA AAAAGAACTCCAAATCAAGCAAGATTCGGTCTCTGGCTGACTATAGAACTGAAGATCCAAGTGACTCTGG TGGCCTTGGTTCTACTGCTGATGCTGTTGGAAGTTCCCTGAAACAAAGTAGAAGTAGCACTTCAGTTGTG TCTGAGGTTAGCCCATCATCTGAGACTGATAACCGTGTAGAGAGTGCCTCCATGACTGGGGATAGTGTGT CAGAGGCTGATGGAAATGAGAGTGACAGTTCCTCACACAGCAGCCTCTCTGCCCGAGGGGCCTGTGGTGT CCTGGGGAACGTGGGCATGCCAGGAACAGCTTACATGGTTGATGGCCAGGAGATTTCTGCTGAGGCCCTG GGCCAGTTTCCTTCAATTAAAGACGTACTACAGGCTGCAGCAGCCCAGCACCAAGATCAGAACCAGGAAG CCAACGGAGAGGTGCGGAGCCGTAGAGACAGCATCTGCAGCAGTGTGTCCATGGAGAGCTCCTTGGCTGA ACCACAGGATGAATTGTTGCAGATTCTTAAAGATAAAAGGAGGCTTGAAGGACAGGTGGAGGCTTTGTCA TTAGAAGCCAGTCAGGCACTTCAAGAGAAGGCTGAGCTACAGGCCCAGCTTGCTGCCCTGAGCACAAGGC TACAGGCACAGGTGGAACACAGCCACAGCAGCCAGCAGAAGCAAGACTCCCTTAGTTCTGAAGTGGACAC TTTGAAGCAGTCTTGCTGGGATCTAGAGCGGGCCATGACTGACCTGCAGAGCATGCTAGAAGCTAAAAAT GCCAGCCTGGCATCCTCAAACAATGACCTACAAGTGGCAGAGGAGCAGTATCAGAGACTGATGGCCAAAG TGGAGGACATGCAGAGAAACATCCTCAGCAAGGACAACACAGTGCATGACCTGCGGCAGCAGATGACAGC GCTACAGAGCCAGCTCCAGCAAGTACAGCTGGAGCGCACAACCCTGACCAGCAAACTACAAGCATCACAG GCTGAGATCACGTCTCTGCAGCATGCCCGGCAATGGTACCAACAGCAGCTTACTTTGGCCCAGGAGGCCA GGGTCAGGCTGCAAGGAGAGATGGCCCATATCCAGGTTGGACAAATGACCCAGGCAGGCCTTTTGGAGCA CCTGAAGCTTGAGAATGTGTCCCTATCGCATCAGCTGACAGAAACTCAGCATCGATCCATCAAGGAGAAA GAGCGCATAGCTGTTCAGCTCCAGAGCATTGAGGCTGACATGTTAGATCAGGAGGCAGCATTTGTACAGA TTCGGGAGGCGAAGACAATGGTGGAGGAGGACCTCCAAAGGAGGCTGGAAGAATTTGAAGGTGAACGGGA GCAGTTACAGAAAGTGGCAGATGCAGCAGCATCCCTGGAGCAACAGTTGGAACAGGTGAAGCTGACATTG TTCCAGCGAGATCAGCAGCTTGCAGCACTTCAGCAGGAGCACCTAGATGTGATAAAGCAGCTTACGTCGA CGCAGGAGGCTCTGCAGGCCAAAGGGCAGTCTCTTGATGACCTGCATACACGTTACGATGAGCTACAAGC AAGGCTGGAGGAGCTGCAGAGAGAGGCCGACTCCAGGGAGGATGCAATTCACTTCTTGCAGAATGAGAAG ATTGTCTTGGAAGTGGCTCTACAATCGGCCAAGAGTGACAAGGAGGAACTTGACAGAGGAGCACGGCGTT TGGAAGAAGATACTGAGGAGACATCAGGACTTTTGGAACAATTGAGACAAGATTTGGCTGTCAAGTCCAA CCAGGTGGAGCACTTGCAGCAGGAGACAGCCACGTTGAGAAAACAGATGCAGAAAGTAAAGGAGCAGTTT GTTCAGCAAAAGGTGATGGTAGAGGCATACCGGCGAGATGCTACTTCCAAAGATCAACTCATCAATGAAC TGAAAGCCACCAAAAAACGGCTGGACTCTGAGATGAAGGAACTGAGGCAAGAGCTAATCAAGCTGCAGGG AGAGAAGAAGACTGTGGAAGTAGAGCACTCACGACTGCAGAAGGACATGTCCCTGGTTCACCAGCAGATG GCAGAGCTTGAGGGGCATCTTCAGTCAGTGCAGAAAGAACGAGATGAAATGGAGATTCACCTGCAGTCTT TGAAGTTTGACAAAGAGCAGATGATAGCCCTTACCGAGGCCAATGAGACACTGAAGAAACAAATAGAAGA GCTGCAACAAGAGGCCAAGAAGGCCATCACAGAACAGAAGCAGAAGATGAAGAGGCTGGGCTCAGATCTG TCCAGCGCCCAGAAGGAGATGAAGACTAAGCACAAAGCTTATGAGAATGCTGTGAGCATTCTCAGCCGTC GCCTGCAGGAGGCTCTGGCTTCTAAGGAGGCTACAGATGCAGAGCTGAACCAGCTCAGAGCTCAGAGCAC AGGTGGCAGCAGTGACCCTGTCCTACATGAGAAGATTCGTGCTCTGGAGGTGGAACTGCAGAATGTTGGC CAAAGCAAGATACTACTGGAGAAGGAGTTACAGGAGGTGATCACAATGACTAGCCAGGAGCTGGAAGAGT CCCGGGAGAAGGTGCTGGAGCTAGAAGATGAGCTTCAAGAATCCAGAGGCTTCAGAAGGAAGATAAAACG TCTTGAAGAGTCAAATAAGAAACTAGCTCTGGAGTTAGAGCATGAGCGAGGGAAGCTCACTGGCCTTGGA CAATCTAATGCAGCGCTGCGGGAGCACAACAGCATTCTGGAGACTGCACTAGCCAAGAGGGAAGCTGACC TGGTACAGCTGAATCTGCAGGTGCAGGCAGTTTTACAGCGCAAAGAGGAGGAGGATCGCCAGATGAAGCA ACTTGTCCAAGCCTTACAAGTCTCACTAGAGAAAGAAAAGATGGAAGTGAACAGCCTGAAGGAACAGATG GCTGCTGCCAGGATAGAAGCTGGGCACAACCGCCGCCACTTTAAGGCAGCTACTTTGGAGCTGAGTGAGG TGAAGAAAGAATTGCAGGCCAAAGAACACCTGGTACAGACATTGCAGGCCGAGGTGGATGAGCTCCAGAT TCAGGATGGAAAGCATTCTCAAGAAATAGCACAATTCCAGACTGAGCTGGCAGAAGCTAGAACCCAGCTC CAGCTCCTACAGAAGAAGCTAGATGAGCAGATGAGCCAGCAACCGACAGGGAGCCAAGAGATGGAAGATC TCAAATGGGAATTGGATCAGAAAGAACGAGAGATTCAGTCCCTGAAGCAGCAGCTTGACTTGACAGAGCA ACAGGGCAAGAAAGAACTGGAGGGGACACAGCAGACACTTCAGACCATCAAGTCTGAGTTGGAAATGGTA CAAGAAGATCTGTCTGAGACGCAGAAGGATAAGTTTATGCTTCAAGCCAAAGTGTCTGAACTGAAGAACA ACATGAAGACTCTGCTGCAGCAGAACCAACAGCTCAAGCTGGATCTCCGCCGCGGTGCAGCCAAGAAGAA AGAGCCAAAAGGTGAGTCCAACTCATCCAGTCCTGCAACACCCATCAAGATACCAGATTGCCCTGTCCCA GCCTCATTGCTAGAGGAGCTGCTGAGGCCCCCACCCGCTGTGAGCAAGGAACCCCTCAAGAATCTCAACA ACTGTTTGCAGCAGCTCAAGCAGGAAATGGACAGTCTCCAGCGCCAGATGGAGGAGCATACCATCACTGT GCATGAGTCCCTGTCCTCGTGGGCTCAGGTGGAAGCTGCCCCAGCAGAGCATGCCCATCCCCGGGGAGAC ACAAAACTGCATAATCAAAACAGTGTTCCCAGAGACGGGCTGGGCCAATGACCACTGTTGTCCTCATCAC ACAAGTATTTGTGACTGTTGAAGGAATTCTCTTGTCAATATTATTTATTTGATCGAAAAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:5709074 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5709074 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19672 same embryo
	EMAGE:19674 same embryo
	EMAGE:19676 same embryo
	EMAGE:19675 same embryo
	EurExpress:euxassay_006124 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source