EMAGE mouse gene expression spatial database

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EMAGE:19674

Ap2b1 adaptor-related protein complex 2, beta 1 subunit ( MGI:1919020)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006109. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006109_01	euxassay_006109_02	euxassay_006109_03	euxassay_006109_04

euxassay_006109_05	euxassay_006109_06	euxassay_006109_07	euxassay_006109_08	euxassay_006109_09

euxassay_006109_10	euxassay_006109_11	euxassay_006109_12	euxassay_006109_13	euxassay_006109_14

euxassay_006109_15	euxassay_006109_16	euxassay_006109_17	euxassay_006109_18	euxassay_006109_19

euxassay_006109_20	euxassay_006109_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19674_wholemount_strong.wlz

19674_wholemount_moderate.wlz

19674_wholemount_weak.wlz

19674_wholemount_possible.wlz

19674_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19674_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8543
Entity Detected:	Ap2b1, adaptor-related protein complex 2, beta 1 subunit ( MGI:1919020)
Sequence:	sense strand is shown >T8543 CGCAGTGGCTTAGATCTAGAGAGAATCGTGACGGGCAGGAAACCATTACACCACCACCCCGGCTGTGCTC TCCGACTGCCGCCGCCGCCGCCGCCACCCCGCCCTTGCCTCCGGTTCACGCTAAAGATCCAGAATCATGA CTGACTCCAAGTACTTCACAACCAATAAGAAGGGAGAAATTTTTGAATTAAAAGCTGAACTCAACAATGA AAAGAAAGAAAAGAGGAAGGAGGCTGTGAAGAAAGTGATTGCTGCTATGACCGTGGGGAAGGACGTGAGC TCTCTCTTCCCAGATGTGGTGAACTGCATGCAGACTGACAACCTGGAACTAAAGAAGCTCGTGTACCTCT ATCTGATGAACTATGCCAAGAGTCAGCCAGACATGGCCATCATGGCTGTCAACAGCTTTGTGAAGGATTG TGAAGATCCCAATCCTTTGATTCGAGCCTTGGCAGTTAGAACCATGGGATGCATCCGGGTGGACAAGATT ACAGAGTATCTCTGTGAACCCCTCCGCAAGTGCTTGAAGGATGAAGACCCCTATGTTCGGAAAACGGCAG CAGTATGCGTGGCAAAACTCCATGATATCAATGCCCAGATGGTGGAAGATCAGGGATTTCTGGATTCTCT GCGGGATCTCATAGCAGATTCAAACCCAATGGTGGTGGCTAATGCTGTAGCAGCATTGTCTGAGATCAGT GAGTCTCACCCAAACAGCAACTTACTTGATCTGAACCCTCAGAATATCAATAAGCTGCTCACAGCCCTGA ATGAATGCACTGAATGGGGCCAGATTTTCATCCTGGACTGCCTGTCTAATTACAACCCTAAAGATGACCG GGAAGCTCAGAGCATCTGTGAGCGAGTAACGCCTCGGCTCTCCCACGCCAACTCTGCAGTGGTGCTTTCA GCCGTAAAAGTCCTAATGAAGTTTCTAGAATTGTTGCCCAAGGACTCTGACTACTACAATATGCTGCTAA AGAAGTTAGCACCTCCACTTGTCACGTTGCTGTCTGGGGAGCCAGAAGTACAGTACGTCGCCCTGAGGAA CATCAACCTAATTGTCCAGAAAAGGCCTGAAATCTTGAAGCAGGAAATCAAGGTCTTCTTTGTGAAGTAC AATGATCCTATCTATGTTAAACTAGAGAAGTTAGACATCATGATTCGTCTTGCATCCCAAGCCAACATTG CTCAGGTTCTGGCGGAGCTGAAGGAATATGCCACTGAAGTTGATGTGGACTTTGTTCGCAAAGCTGTGAG GGCCATTGGACGGTGTGCCATCAAAGTGGAGCAATCAGCAGAACGCTGTGTAAGCACACTGCTTGATCTA ATCCAGACCAAAGTAAATTATGTGGTCCAAGAGGCAATTGTTGTCATCAGGGACATCTTCCGAAAATACC CCAACAAGTATGAAAGTATTATTGCCACCCTCTGTGAAAACTTGGACTCCCTGGATGAACCCGATGCCCG AGCAGCTATGATTTGGATTGTAGGAGAATATGCTGAAAGAATCGATAACGCAGATGAATTACTAGAGAGT TTCCTGGAAGGTTTTCATGACGAAAGCACCCAGGTGCAGCTCACACTGCTCACCGCCATAGTGAAGCTGT TTCTCAAGAAGCCATCAGAAACACAGGAGCTGGTCCAACAGGTCTTGAGCTTAGCCACACAGGACTCCGA TAACCCTGACCTTCGAGATCGGGGTTATATATATTGGCGCCTTCTTTCAACTGACCCTGTGACAGCCAAA GAAGTGGTGTTGTCTGAGAAGCCATTGATCTCTGAGGAGACAGACCTCATTGAGCCTACCCTCCTGGATG AGCTCATCTGCCACATTGGTTCTTTGGCCTCTGTGTACCACAAACCTCCAAATGCTTTTGTGGAAGGGAG TCATGGGATTCATCGCAAACACTTGCCAATTCATCATGGGAGCACTGATGCAGGTGATAGCCCCGTTGGC ACCACCACTACAACCAACCTGGAGCAGCCTCAGGTCATTCCCTCTCAAGGTGACCTTCTGGGGGATCTTT TGAATCTTGACCTGGGTCCCCCAGTGAATGTCCCGCAAGTGTCCTCCATGCAGATGGGAGCGGTGGATCT TTTAGGAGGAGGACTGGATAGCCTGCTTGGCAGTGACCTTGGCGGGGGCATTGGAGGAAGTCCGGCAGTA GGACAGTCCTTCATCCCGTCATCAGTGCCTGCGACCTTCGCTCCTTCACCTACTCCTGCTGTGGTCAGCA GTGGTCTGAATGACCTGTTTGAGCTTTCCACTGGGATAGGCATGGCACCTGGCGGATATGTGGCTCCTAA GGCGGTCTGGCTACCTGCAGTAAAGGCTAAAGGCTTGGAGATTTCGGGGACGTTTACTCACCGCCAAGGG CATATCTATATGGAAATGAACTTCACCAACAAAGCTCTGCAGCATATGACAGACTTTGCCATCCAGTTTA ACAAGAATAGCTTCGGTGTCATCCCAAGCACTCCCTTGGCCATACATACTCCACTGATGCCAAACCAGAG CATTGACGTCTCTCTGCCGCTCAACACCTTGGGCCCAGTCATGAAGATGGAGCCCCTGAATAACCTGCAG GTGGCTGTTAAAAACAATATTGATGTCTTCTACTTCAGCTGCCTCATCCCACTCAATGTGCTTTTTGTAG AAGATGGCAAAATGGAGCGCCAGGTCTTCCTTGCAACGTGGAAGGATATTCCCAATGAAAATGAACTCCA GTTTCAGATTAAGGAATGTCATTTAAATGCTGACACAGTTTCCAGCAAGTTGCAAAACAACAATGTTTAC ACTATTGCCAAGAGGAACGTGGAAGGGCAGGACATGCTGTACCAGTCCCTGAAGCTCACTAATGGCATTT GGATTTTGGCAGAGCTGCGGATCCAGCCAGGAAACCCCAATTATACGCTGTCGCTGAAGTGTAGAGCCCC TGAAGTCTCTCAGTACATCTATCAGGTCTACGACAGCATTTTGAAAAACTAATAAATGGGTCCAGTCAGC CTGTAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5708062 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5708062 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19672 same embryo
	EMAGE:19676 same embryo
	EMAGE:19673 same embryo
	EMAGE:19675 same embryo
	EurExpress:euxassay_006109 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source