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EMAGE:19693

Hist1h2ae histone cluster 1, H2ae ( MGI:2448290)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19693 EMAGE:19693 EMAGE:19693 EMAGE:19693 EMAGE:19693
"Pseudo-wholemount" of euxassay_006147. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006147_01 euxassay_006147_02 euxassay_006147_03 euxassay_006147_04
EMAGE:19693 EMAGE:19693 EMAGE:19693 EMAGE:19693 EMAGE:19693
euxassay_006147_05 euxassay_006147_06 euxassay_006147_07 euxassay_006147_08 euxassay_006147_09
EMAGE:19693 EMAGE:19693 EMAGE:19693 EMAGE:19693 EMAGE:19693
euxassay_006147_10 euxassay_006147_11 euxassay_006147_12 euxassay_006147_13 euxassay_006147_14
EMAGE:19693 EMAGE:19693 EMAGE:19693 EMAGE:19693 EMAGE:19693
euxassay_006147_15 euxassay_006147_16 euxassay_006147_17 euxassay_006147_18 euxassay_006147_19
EMAGE:19693 EMAGE:19693 EMAGE:19693 EMAGE:19693 EMAGE:19693
euxassay_006147_20 euxassay_006147_21 euxassay_006147_22 euxassay_006147_23 euxassay_006147_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19693Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19693_wholemount_strong.wlz
19693_wholemount_moderate.wlz
19693_wholemount_weak.wlz
19693_wholemount_possible.wlz
19693_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19693_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8570
Entity Detected:Hist1h2ae, histone cluster 1, H2ae ( MGI:2448290)
Sequence:sense strand is shown

>T8570
GCAATTATAATTTCCTACCTTCTTGCGTCAATGTCTGGACGTGGCAAACAGGGTGGCAAGGCTCGCGCCA
AGGCCAAGACCCGCTCCTCCCGGGCCGGCCTGCAGTTTCCCGTGGGCCGCGTGCACCGGCTGCTCCGCAA
AGGCAACTACTCGGAGCGCGTGGGCGCCGGCGCCCCGGTGTACCTGGCGGCCGTGCTGGAGTACCTGACG
GCCGAGATCCTGGAGCTGGCGGGCAACGCGGCCCGCGACAACAAGAAGACGCGCATCATCCCGCGCCACC
TGCAGCTGGCCATCCGCAACGACGAGGAGCTCAACAAGCTGCTGGGCCGCGTGACCATCGCGCAGGGCGG
CGTCCTGCCCAACATCCAGGCCGTGCTGCTGCCCAAGAAGACCGAGAGCCACCACAAGGCCAAGGGGAAA
TAAGATCTTTCTTCTAATCTCGTAAAAAAAACAAAGGCTCTTTTCAGAGCCATTCAAAAAAAAAAAAAAA
AA
Notes:The probe template was PCR amplified from IMAGE:5713252 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5713252 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19696 same embryo
 EMAGE:19692 same embryo
 EMAGE:19694 same embryo
 EMAGE:19695 same embryo
 EurExpress:euxassay_006147 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS