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EMAGE:19694

Pitpna phosphatidylinositol transfer protein, alpha ( MGI:99887)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19694 EMAGE:19694 EMAGE:19694 EMAGE:19694 EMAGE:19694
"Pseudo-wholemount" of euxassay_006148. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006148_01 euxassay_006148_02 euxassay_006148_03 euxassay_006148_04
EMAGE:19694 EMAGE:19694 EMAGE:19694 EMAGE:19694 EMAGE:19694
euxassay_006148_05 euxassay_006148_06 euxassay_006148_07 euxassay_006148_08 euxassay_006148_09
EMAGE:19694 EMAGE:19694 EMAGE:19694 EMAGE:19694 EMAGE:19694
euxassay_006148_10 euxassay_006148_11 euxassay_006148_12 euxassay_006148_13 euxassay_006148_14
EMAGE:19694 EMAGE:19694 EMAGE:19694 EMAGE:19694 EMAGE:19694
euxassay_006148_15 euxassay_006148_16 euxassay_006148_17 euxassay_006148_18 euxassay_006148_19
EMAGE:19694 EMAGE:19694 EMAGE:19694 EMAGE:19694 EMAGE:19694
euxassay_006148_20 euxassay_006148_21 euxassay_006148_22 euxassay_006148_23 euxassay_006148_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19694Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19694_wholemount_strong.wlz
19694_wholemount_moderate.wlz
19694_wholemount_weak.wlz
19694_wholemount_possible.wlz
19694_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19694_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8566
Entity Detected:Pitpna, phosphatidylinositol transfer protein, alpha ( MGI:99887)
Sequence:sense strand is shown

>T8566
GAGTCCAGGGCAGGGCGACAGGAGCCCGCGCGGCGACCGCAGCGAGAGCAGCGGGGACAGAGCAGACCAC
GACGAAGGCGCACAGGCAGCGGGGCCGGGCCGGGCCACCGGGAGAGCGGCCACCGGGTAGCGGGCGGGAG
GCCGGGCTGGCAGCTGGCAGCCGCCGAGCCGCGAAGCGACATGGTGCTGCTCAAGGAGTATCGGGTCATC
CTGCCTGTGTCTGTAGATGAGTATCAAGTGGGGCAGCTGTATTCTGTGGCTGAAGCCAGTAAAAATGAAA
CTGGTGGTGGGGAAGGTGTGGAGGTCCTGGTGAATGAGCCCTACGAGAAGGACGATGGCGAGAAAGGCCA
GTACACACACAAGATCTACCACTTACAGAGCAAAGTACCCACCTTTGTTCGAATGCTGGCCCCAGAAGGC
GCCCTGAATATACACGAGAAAGCCTGGAATGCCTACCCTTACTGCAGAACCGTTATTACAAATGAGTACA
TGAAAGAAGATTTTCTGATTAAAATTGAAACCTGGCACAAGCCAGACCTTGGCACCCAGGAGAATGTGCA
TAAACTGGAGCCTGAGGCATGGAAACATGTGGAAGCTATATATATAGACATCGCTGATCGAAGCCAAGTA
CTTAGCAAGGATTACAAGGCAGAGGAAGACCCAGCAAAATTTAAATCTGTCAAAACAGGACGCGGACCTT
TGGGCCCGAATTGGAAGCAAGAACTTGTCAATCAGAAAGACTGCCCATATATGTGTGCATACAAACTGGT
TACTGTCAAGTTCAAGTGGTGGGGCTTGCAGAACAAAGTGGAAAACTTTATACATAAGCAAGAGAAGCGT
CTGTTTACAAACTTTCACAGGCAGCTGTTCTGTTGGCTCGATAAATGGGTTGATCTGACTATGGATGACA
TTCGGAGGATGGAAGAAGAGACGAAGAGACAGCTGGATGAGATGAGACAAAAGGACCCAGTGAAAGGAAT
GACAGCAGACGACTAGCGCCACCGCCCTTTCTGCACTTTTTGCAAGACAGTGGTCAACAGGAAGGCCCAG
CAGCTCCACCCTCCTGAGTGGCAGGCCATCTTCGGACGCAGCCTTTCTGTGCTCATTCTTCAGGCAACTT
TCAATCCCTTACTATAGCTATTTCTAGATGCCCTTTAACATTGTGAACAAATAGACCGTCTGGTATTATA
AAGCCTGTGTGTGCGGGAGCCTGATCCTCTAAGATACATGGTGTAGCTGCTGTGTTTACAGCTCATCCTC
TCTCCACTGTGCTCTGACCCATTTCTGTATGCCCTCCCCTTTTATTTCCAAGTGATGGTCTCAGTCTTCC
CAATGAGCTGCCTTTTGTGATGTGATTATTTAAGCTCATTTATTTCAGCCTTGGGATAAACTGAGCTATT
TTGCTTCCTGGGCAGATCTTTGGCAATGCTGAATATTCACTTGGGGAGAGAGGAGGAGTTAAAAATACAG
AGACACCTACCCCCAGCTCCTCACAATAGGAATGTGGCTTCAGACCCCTGACCTCTGCTCCTAGTAACCT
CTTGTTTGCGTGCTCTAAGCACCAAGAAGGCACCCAAGGCCCAGCCAGTCTCAGAAAATTTAGCAAATAA
TAAATAACCCCTACTCGAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5710926 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5710926 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19696 same embryo
 EMAGE:19692 same embryo
 EMAGE:19693 same embryo
 EMAGE:19695 same embryo
 EurExpress:euxassay_006148 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS