EMAGE mouse gene expression spatial database

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EMAGE:19713

Rgl2 ral guanine nucleotide dissociation stimulator-like 2 ( MGI:107483)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006228. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006228_01	euxassay_006228_02	euxassay_006228_03	euxassay_006228_04

euxassay_006228_05	euxassay_006228_06	euxassay_006228_07	euxassay_006228_08	euxassay_006228_09

euxassay_006228_10	euxassay_006228_11	euxassay_006228_12	euxassay_006228_13	euxassay_006228_14

euxassay_006228_15	euxassay_006228_16	euxassay_006228_17	euxassay_006228_18	euxassay_006228_19

euxassay_006228_20	euxassay_006228_21	euxassay_006228_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19713_wholemount_strong.wlz

19713_wholemount_moderate.wlz

19713_wholemount_weak.wlz

19713_wholemount_possible.wlz

19713_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19713_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 06 weak expression: see section 07 08 09 20 21 22

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 06 16 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 16 17 18 19 20

vagus x ganglion

moderate

regional

moderate expression: see section 07 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 16

cervico-thoracic ganglion

weak

regional

weak expression: see section 08 14

cervical ganglion

weak

regional

weak expression: see section 08 15

thoracic ganglion

weak

regional

weak expression: see section 09 10

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8898
Entity Detected:	Rgl2, ral guanine nucleotide dissociation stimulator-like 2 ( MGI:107483)
Sequence:	sense strand is shown >T8898 GGAAGCGGAAAGGGCGGGAGGGCCCGGGAGCCCGGCGGGGTGCAGGAGGGGGGCATCGCGGAAAAGCCGG GGAGGGGACTCGGTAGAGGGCCAGAGACCGACAAGAACAGAGGCCCGGGACCCGCGCGCAGCCCTCGCCC TTCCCGAGCAGGCGCCCCCATGGCCCGACCCCGCTGATTGCTTCTCTCGGCCATGCTCCCGCGGCCCCTG CGGCTGCTTTTGGACACGACCCCCCCCGGGGGAGTCGTGCTGAGCAGCTTCCGGAGCCGGGACCCCGAAG AGGGTGGGGACCCAGGTGGCCGGGCCGTGGGCGGGGGGCAGGAGGAAGAGGATGAGGAAGAAGAAGAGGC TTCTGTGTCAGTCTGGGACGAGGAGGAGGATGGTGCGACCTTTACTGTCACAAGCCGCCAGTACAGGCCT CTTGACCCCTTGGCTCCCTTGCCTCCACCTCGCTCCTCCCGACGGCTCCGCGCTGGCACTCTGGAGGCCC TGGTCAGACACCTCTTGGATGCCAGGACAGCAGGGGCTGACATGATGTTCACTCCGGCCTTGCTGGCCAC CCACCGGGCCTTCACCTCCACTCCTGCCCTGTTTGGGCTTGTGGCTGACAGGCTGGAAGCCCTTGAATCT CATCCTCCCGGTGAGCTAGAGAGGACCACAGGGGTAGCCATCTCTGTACTTTCAACTTGGCTGGCCTCTC ACCCTGAGGATTTTGGCTCTGAGGTCAAGGGTCAACTTGACCGGCTTGAGAGCTTCTTGCTTCGGACAGG GTATGCAGCACGGGAGGGTGTTGTGGGGGGCAGTGCTGACCTCATCCGAAACCTCCGGGCCCGGGTGGAC CCCCGGGCCCCCGACCTTCCTAAGCCCCTGGCCCTTCCTGGCGATTCCCCTGCTGACCCCACGGATGTCC TGGTGTTCCTCGCTGACCACTTGGCCGAACAGCTGACCCTGCTAGATGCGGAACTGTTTCTTAATCTGAT CCCCTCTCAGTGTTTGGGAGGCCTCTGGGGTCACAGAGACCGGCCAGGACATTCTCACCTCTGCCCGTCT GTCCGAGCTACCGTCACACAGTTCAACAAGGTGGCGGGGGCGGTAGTTAGCTCTGTCTTGGGGGCCACCT CAATTGGAGAGGGGCCAAGAGAGGTGACTGTGAGACCACTGCGGCCCCCACAGAGGGCCCGGCTCCTAGA GAAGTGGATCCGTGTGGCCGAGGAGTGCCGCCTGCTTCGGAACTTCTCCTCAGTGTATGCTGTTGTGTCC GCTCTGCAGTCCAGCCCTATCCACAGGCTTCGGGCAGCCTGGGGGGAGACAACCAGGGACAGCCTCCGAG TCTTTTCCAGCCTGTGCCAGATTTTCTCAGAGGAGGATAATTATTCCCAGAGCAGAGAGCTCCTCATGCA GGAAGTGAAGCCGCAGCCCCCTGTGGAGCCACACTCCAAGAAGGCCCCAAGGTCTGGCTTCAGGGGTGGG GGTGTGGTTCCCTACCTGGGAACCTTCCTGAAGGACCTCGTGATGCTGGATGCGGCCTCCAAGGATGAGC TGGAGAATGGCTACATCAATTTTGACAAGCGGAGGAAGGAGTTTGCTATCCTTTCGGAGCTGTTGCGCCT CCAGAAAGAATGTCGTGGCTACGACCTCCGACCTAACTCTGATATCCAGCAATGGCTCCAGGGCCTCCAG CCTTTAACTGAAGCTCAGAGTCACCGTGTATCCTGTGAAGTGGAGCCACCAGGGACCAGTGACTCCCCCG CTGCAAGGACACCTCGGCCAACACTAGTGATCACACAGTGGACGGAAGTTCTGGGCTCTGTTGGAGGCCC CACTCCGCTTGTGTCCTGGGATCGGCCCAGTGTCGGGGGAGATGAGGTGCCTGGAACCCCAGCACCTCTG CTGACTCGCCTCGCCCAGCACATGAAGTGGCCATCAGTCTCATCTCTGGACTCTGCCCTGGAAAGCAGCC CCTCCTTGCACAGCCCTGCTGACCCTGGCCACCTCTCTCCTCCAGCCTCCTCCCCTAGGCCTTCCCGGGG TCACCGTCGCTCAGCCTCCTGTGGGTCTCCGTTGAGTGGAAACACAGGAGAAGGGACCTCTAGGAGTGCT GGATGTGGGGGCGGGGTATCTGGGCCAGGGTCCTCTGATTGCCGAATCATCCGAGTCCAGATGGAGCTGG GGGAGGATGGCAGCGTCTACAAGAGCATCCTGGTGACAAGCCAGGACAAAGCTCCAAGTGTTATTAGTCG TGTCCTTAAGAAAAACAATCGTGATTCTGCTGTGGCTTCAGAGTTCGAGCTGGTGCAGCTGCTACCTGGG GATCGAGAGCTGACCATCCCACACTCAGCTAACGTCTTCTATGCCATGGATGGTGCATCTCATGACTTCC TCCTGCGGCAGCGCAGAAGACCCTCTGCTGCCACCCCGGGTTCCCACAGCGGCCCCTCTGCCTCAGGAAC TCCTCCGAGCGAGGGGGGAGGGGGCTCCTTTCCCAGGATCAAGGCCACGGGGAGGAAGATTGCACGGGCA CTGTTCTGAGGATGAAGTCCTATTGGTTTACATAGCAACTCATACCAGGAGTGAGCAGCCACACATGGTG GCCACAATATTAGAGCAGAAACCCTGAAAGGTGGCCAGCCACCCTGGGACAGTGAAGTCACCTGTTTACG AAACCACTGCGGAAGCCAGCCCCAGCCCCAGCCCCGTGGGACTGGTGACCACGTAACCATGTTGGATACC TGCATCTAGCTTCCACCTCTGCGGAATGTGGCATTATCATCTGTGCTGGGAAGAGTACTGGTCCCTGCCA CATGCCAGCCTGTGACAGGCCAAGGGAAGGCCTAGAGGCTGAGCCTGGGGCAGAGGGCAGTGACAAGAGG TTCTTGTCCCACTCTGGGGATTCCTGTCACTGTGACAACACTAAGAAAATAAACCAAAACACTACCTGAA AAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6415928 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6415928 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19716 same embryo
	EMAGE:19711 same embryo
	EMAGE:19715 same embryo
	EMAGE:19714 same embryo
	EMAGE:19712 same embryo
	EurExpress:euxassay_006228 same experiment
	MGI:4827711 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source