EMAGE mouse gene expression spatial database

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EMAGE:19716

1810032O08Rik RIKEN cDNA 1810032O08 gene ( MGI:1913543)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006153. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006153_01	euxassay_006153_02	euxassay_006153_03	euxassay_006153_04

euxassay_006153_05	euxassay_006153_06	euxassay_006153_07	euxassay_006153_08	euxassay_006153_09

euxassay_006153_10	euxassay_006153_11	euxassay_006153_12	euxassay_006153_13	euxassay_006153_14

euxassay_006153_15	euxassay_006153_16	euxassay_006153_17	euxassay_006153_18	euxassay_006153_19

euxassay_006153_20	euxassay_006153_21	euxassay_006153_22	euxassay_006153_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19716_wholemount_strong.wlz

19716_wholemount_moderate.wlz

19716_wholemount_weak.wlz

19716_wholemount_possible.wlz

19716_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19716_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8866
Entity Detected:	1810032O08Rik, RIKEN cDNA 1810032O08 gene ( MGI:1913543)
Sequence:	sense strand is shown >T8866 CGAGGTCCTCTGCCGCGTGTTTGGAAGTTATGGCCGCGCGGGTCTGTGGTGTTCTCCCTGAAAGGCCTAA GCTTTGGGTGATGGCATTGCCTTTTGGCACTCGGGAGACAAGATGCAGGTTCCGTCTCAGAAGTAATGGC TGGATCCCTGAAAGCCAACGGGCTCGGTGTGGTCCACCCGTCAGAAGGCATAGGTAGATGGTGGGCATCT GCCTCAGACATAGTTCTCAAGTTCAGCCAGAAGAATGCGCGTTGAGCCCTCTGCAGCCCGTGTCGACGGA GGTGTTGTCTCCAGGAAGCATCATGGAAAGGCGTGGTGGTGCACAAGGGCCTTCAGGATGCCTGCGCCTG GTGACGGCTGCGCCTCAGGTTCTGGGCCTGACTCGGAAGGGTGCCTGTGAGCAGAAGAGCTGGCACGCGC AGCTCAGTGGACCATCAGTGCCAACCCGTCCTGTACCCTGGCTGAGGGGTGTGCTAAGTGGCAGATTCCC GGTTGGCTCATCGTAAGGCGTGGCACAAAATCACCATCGAAAGAAAGGGCCTTCGGGATGCCTCTATCTG GTAAGAGCTTCACAGAATTGCAGGGGTTGTGGTTGCTGGGAAGGGTTAGTCTGTTAGATCAGGTGCTGCA GAATCGAGTGTAACCCTTCCTCCCTTTAGTCTTACTGTGTAGCCCGTGCTGGCCTGCAACTTGCTATGTA GACCTGGTTGACCTCAAACTCAGTTTGTGGCCTTTCAGGTGCTGGGATTTTCATGTTCTCATTTTGTGTT CCCGAAAAGCCCATAAGCCTATGATGGATTGGTTATCCCTGTCTGAAGATTTCAGCTGAGGGAAAATACT CTATTCTGAGGCTTAGGGGTGTGGCTCCATGCTTGAAGAAATTTGAGCTTTATAAGGGATCAAGTTAAAT AGACTCCATAGAGAGCTGTGTGCCAACCTCCCAAAACCTAAGGTCTAGTTGCCTGTTAATCCTCCTCCTT GGGTGCTCTGAGGGCAAAGTCCCTGAGTTAGCAGAAATGGGCGGGCTGCCAGCACCTGTCCTGTGCTCAC TGGAGTGAGACATGCTGGTCACATAGTTCTAGAAAATGAATGTCTTCCTTTAGAGGAGGCAGGGATGTAA GGCGTGCCATGAGAATGCTTGACGACTCTGTCGTTGCCTCTAGCGGTGCTGACATTTAGAAAAGAAGTTG TGGCCCTGGCAGCCTCCTAAGTATCGTCTCCTTGATGGCACAGAAACAAGTGGCGGGCTTGGGGGGGGGG GGGCTTTGCTCAGCCCAGCCTTAGTAATGCAGTGTACACTGTGTCCTTTTTCTCTATGGAATCTGGGGGG TGGATATGGGCTTACACTAAAGTAGCTGTACTTGTGGAATTCTCTGCAGGGTGGCTCTGCTTGCCTTCAC TTCTGAGTGGACTCAGGTTCAGGTCCTCAGGGATTTGGTTGAATATAAATACTCTGATGTTTCATGAGAA ACCTTTTTTTGGCCACTTACCAGGAACTTCTTAAAACTTTCTTTTAAAAAAAAAGTTAGCAGCCTGCTTC TGAACTTGGCTGGTGCCTGCCTGTGTGTAGCGCACACAGTGCCTTGTGATGGCGGCTGGAATCCCAGGAC TATCTGATAATGCTTGTTCCTAAAGTTTGTAGTTTTGATCACTTAGCTGCCTGTCTCCTTCAGGTTCTTT CCTTGTTTCTAGGTTATTGCCCTGTAGTGCCAGAGAGAACCACATAGCCTGCTGCTGCTCCTGCCTTTAA TTTCCCAAAGAGTCATCAGACGACCTTGGAGTTTGTTCTGCAAGAGGAGCCAGTAAGGCCTCGTGACTGT GGTCACCCTCGGAAGCCTTTGACAGCTAGCTGGTGAGGGCGCTCAGTCGGAGGCCCTGGGGAGGCCCCTG CTAAGCCGAGAAAAGCATTTCTCACAGTGGATTTCCTGGAAGTCAGGGTTGGCCTTTGTATTTTGAAGCA CAAATAAACTTTATAAAATTTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6414099 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6414099 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19711 same embryo
	EMAGE:19715 same embryo
	EMAGE:19714 same embryo
	EMAGE:19712 same embryo
	EMAGE:19713 same embryo
	EurExpress:euxassay_006153 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source