EMAGE mouse gene expression spatial database

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EMAGE:19742

Serpina3m serine (or cysteine) peptidase inhibitor, clade A, member 3M ( MGI:98378)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006231. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006231_01	euxassay_006231_02	euxassay_006231_03	euxassay_006231_04

euxassay_006231_05	euxassay_006231_06	euxassay_006231_07	euxassay_006231_08	euxassay_006231_09

euxassay_006231_10	euxassay_006231_11	euxassay_006231_12	euxassay_006231_13	euxassay_006231_14

euxassay_006231_15	euxassay_006231_16	euxassay_006231_17	euxassay_006231_18	euxassay_006231_19

euxassay_006231_20	euxassay_006231_21	euxassay_006231_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19742_wholemount_strong.wlz

19742_wholemount_moderate.wlz

19742_wholemount_weak.wlz

19742_wholemount_possible.wlz

19742_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19742_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

single cell

strong expression: see section 06 07 08 09

medulla oblongata basal plate mantle layer

strong

single cell

strong expression: see section 05 06 07 11 12

pons mantle layer

strong

single cell

strong expression: see section 04 14

facial vii ganglion

moderate

single cell

moderate expression: see section 02 03 17

trigeminal v ganglion

moderate

single cell

moderate expression: see section 01 02 03 04 05 06 15 16 17 18 19

dorsal root ganglion

strong

single cell

strong expression: see section 06 07 10 11 12 13 14

axial skeleton tail region

strong

regional

strong expression: see section 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T677
Entity Detected:	Serpina3m, serine (or cysteine) peptidase inhibitor, clade A, member 3M ( MGI:98378)
Sequence:	sense strand is shown >T677 TCCTCGAGNCTGTTGGCCTACTGGATGCCAGGCAAGCCAACAACCCTGAACATCAGGAGTCAGCTATCAC AGAGGCTCTGCAGACTGCACAGAAGATGGCCTTCATTGCAGCTCTAGGGATCTTAATGGCTGGGATCTGT CCTACTGTCCTCTGCTTCTCAGATGACACATGGGGAATAGATATTCTATTACATAAAAACCAAGAAAGTG GGACACCAGATGACAGTCTCACATTGGCGTCCATCAACACTGACTTTGCCTTCAGCCTGTACAAGAAGAT GGCTTTGAAGAATCCAGATAAAAATATTGTCTTCTCCCCACTTAGCATCTCAGCTGCCTTGGCCCTCGTG TCCCTGGGAGCAAAGGGCAACACCCTGGAAGAGATTTTAGAAGGTCTCAAGTTCAATCTCACAGAGACCT CTGAAGCAGACATCCACCAGGGCTTTGGGCACCTCCTACAGAGGCTCAGCCAGCCAGAAGACCAGGATCA GATAAACATAGGCAATGCCATGTTTATTGAAAAGGACCTGCAGATCCTGGCAGAATTCC
Notes:	The probe template was PCR amplified from IMAGE:1888597 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1888597 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19739 same embryo
	EMAGE:19741 same embryo
	EMAGE:19743 same embryo
	EMAGE:19740 same embryo
	EMAGE:19738 same embryo
	EurExpress:euxassay_006231 same experiment
	MGI:4827981 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source