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EMAGE:19743

Pcdhb22 protocadherin beta 22 ( MGI:2136760)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19743 EMAGE:19743 EMAGE:19743 EMAGE:19743 EMAGE:19743
"Pseudo-wholemount" of euxassay_006195. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006195_01 euxassay_006195_02 euxassay_006195_03 euxassay_006195_04
EMAGE:19743 EMAGE:19743 EMAGE:19743 EMAGE:19743 EMAGE:19743
euxassay_006195_05 euxassay_006195_06 euxassay_006195_07 euxassay_006195_08 euxassay_006195_09
EMAGE:19743 EMAGE:19743 EMAGE:19743 EMAGE:19743 EMAGE:19743
euxassay_006195_10 euxassay_006195_11 euxassay_006195_12 euxassay_006195_13 euxassay_006195_14
EMAGE:19743 EMAGE:19743 EMAGE:19743 EMAGE:19743 EMAGE:19743
euxassay_006195_15 euxassay_006195_16 euxassay_006195_17 euxassay_006195_18 euxassay_006195_19
EMAGE:19743 EMAGE:19743
euxassay_006195_20 euxassay_006195_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19743Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19743_wholemount_strong.wlz
19743_wholemount_moderate.wlz
19743_wholemount_weak.wlz
19743_wholemount_possible.wlz
19743_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19743_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T545
Entity Detected:Pcdhb22, protocadherin beta 22 ( MGI:2136760)
Sequence:sense strand is shown

>T545
TCCTCNAGNCTGTTGGCCTACTGGAGATCCCAGGCAGAGCTCAGACCTTTGAGACTCCAGAGAATGCTGC
TAATCTACACTTGGGAGCAGGCTATTAACTGACAGGTCGACCACTGTCTGGATACCCTTGTACCCTGGGT
GCAGAAGCAAAGATGAAGATTGGAAGGGAACACAGAAAAAGGCAAGTTCTGTTGATCTTTCTCTTGCTGG
GAGTGGTTGGGGCGGGCTCGGAACCCCGCCGCTACTTTGTGATGGAGGAAACACCCAGTGGCACTGTTTT
GGCAGATCTAGTCCAGGACCTAGGGCTGGGAGTTGCGGAGCTAGCTGCTCGAGGAGCCCAGGTAGTCTCT
GAGGAAAAGGAATCCCGCTTGCAGCTGGATCTACAGACTGGGAAGCTAATCTTAAATGAAAAACTGGACC
GCGAGGAGCTGTGCGGCTCCACTGAGCCCTGTGTCACTCATTTCCAAGTGTTACTGAAAAAACCACTGGA
AATATTTCAAGCTGAGCTACGAGTAGGAGACATTAATGATCATTCTCCTGAGTTTCCTGAAAGA
Notes:The probe template was PCR amplified from IMAGE:1499022 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1499022 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19739 same embryo
 EMAGE:19741 same embryo
 EMAGE:19740 same embryo
 EMAGE:19742 same embryo
 EMAGE:19738 same embryo
 EurExpress:euxassay_006195 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS