EMAGE mouse gene expression spatial database

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EMAGE:19766

Trmt2b TRM2 tRNA methyltransferase 2 homolog B (S. cerevisiae) ( MGI:2442530)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006284. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006284_01	euxassay_006284_02	euxassay_006284_03	euxassay_006284_04

euxassay_006284_05	euxassay_006284_06	euxassay_006284_07	euxassay_006284_08	euxassay_006284_09

euxassay_006284_10	euxassay_006284_11	euxassay_006284_12	euxassay_006284_13	euxassay_006284_14

euxassay_006284_15	euxassay_006284_16	euxassay_006284_17	euxassay_006284_18	euxassay_006284_19

euxassay_006284_20	euxassay_006284_21	euxassay_006284_22	euxassay_006284_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19766_wholemount_strong.wlz

19766_wholemount_moderate.wlz

19766_wholemount_weak.wlz

19766_wholemount_possible.wlz

19766_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19766_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 10 11 15 16 17

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8592
Entity Detected:	Trmt2b, TRM2 tRNA methyltransferase 2 homolog B (S. cerevisiae) ( MGI:2442530)
Sequence:	sense strand is shown >T8592 CCCCTTCGCGCCATCCGCTGGTATTTTAGTTTGCCACTGCACGTCCGTCCAAGGCCTTAGTGACTCCCAA GGAGTCCCGGGACATCTGGCTTGTTTCCAGCTTCTGGCTATTACAAATAAGGCTGCTATGAACACAGTGG AGCATGTGTCCTTGTGGCATCAAGAGTCCTAAATGCACAACCCAAGACTATTCCTTTCCAGAGCGGGTTT CTTCTCCAAACCAGGACTGCTTCCCTGGGATACTGGAAGACCACCAGATTTGTCACAGTCCTTATTGGGC AGAGCATATAAAGGGAGTTTCACCAGCGTGATAGCTAAGAAGCACCCAAGCAGACAAAAATATCAGAAGA AACAAAGGCATTGTAGAGATGACTCCTGGCAGGAGAGGCTGGCTGATGTTGTGACTCCACTCTGGAGGCT GAGTTATGAAGCACAACTCAAGGTGAAATTTGAAGCTCAGAAGAAACTTTTACAAAGCCTAGAGTCTCAC CTAAAAGTGCTCCATGGAGTCAGTGACACAGTGGCTGCACATCAGTCTGAGGGGCTCCGCTGTCTCCTCC ATCCCATTATACCCTCTCCTACCACCACTGGCTACCGGAACAAGTCCACCTTTTCTGTGTACAGGAGCCC AGATGGCAATCCAAAGACTGTGGGCTACTACCTAGGAACTTGGAAAGATGGAAATGTCGTCTGTCTACCA TGTAATCATCTGAAAAACATCCCAGAGAAACACAGTCAAGTGGCTCAGTACTATGAAGTATTCCTTCGAC AGTCTTCAGTGGAACCCTGCCTTCTCTTTCATGAAGGTGGACACTGGCGTGAACTCGTAGTTCGTACCAA TAGGCAAGGACACACAATGGCTATTGTCACTTTTCATCCCCAGGGATTAAGTGAGGAGGAGGTCTGTGTT CAGAAGGTGACCTTGAAGGATTTTTTCACTAAAGGCCCTGGAGCGATCTGTGAATTGACCTCGCTCTATT TCCAGGAAAGCACCATGACCCGTTGCAGCCATCAGCAGTCCCCCTACCAGCTCCTGTTTGGGGAGCCCCA CATCTTTGAGGATCTTCTGGGTTTGAAGATTCGAATTTCTCCAGATGCCTTCTTCCAGATTAATACTGCT GGGGCAGAGATGCTGTATCGGATCATTGGGGAGCTGAGTGGAGTGAACTCTGAGAGCCTCCTCCTAGACA TTTGCTGTGGCACGGGTGTGATTGGCCTCTCTGTGGCTCAGCGTGCCTCCCAGGTTCATGGCATTGAGCT AGTGGAGCAGGCAGTAGAGGATGCCAGGTGGACTGCAGCCTTCAATGGTGTCACCAACTGTGAGTTTCAC GCTGGCAGAGCAGAGACGATTTTGCCACAGCTGTTAAAGTCACAGAAAGATGAGAAGTTGACTGTTGCTG TGGTGAACCCTGCCCGAGCTGGATTGCATTACAGAGTGGTGCGAGCCATTCGGAACTGCAGGACCATCCA CACTCTAGTGTTTGTTTCCTGCAAGCCCCATGGTGAAAGCACAAGGAACTTCATTGAACTATGCTGTCCT CCAAATTCTGCAAAGCAGCTCCTAGGTGATCCATTCGTCCTGAGAGAAGCTGTACCTGTGGATTTGTTTC CCCACACCCCACACTGTGAGCTGGTTCTCCTCTTCACTCGGTAAGACCTGGACGCTGCAGGCTGATAAGG CTGAAGTTTCCGAGACACCCAGGTTGCCATAGGAGACAGTGGAAGCATCACCAGAAAGACCAATTTTGAA TCCTGGCCAAAAGAATTTGACTGGCCTCCTTCTCTGAGGCAGATTGGACTGTAGCTTGTCCTAGTCCTTT GGGGTTATCCATATTCCCTTAGCTTTGATTGCTCCTAACTCCTTGAACCTGAATTTGTGTTGCTCCATCC TTTTGCTGTCTATACATCATTCAATCTACAGTCTTGTCCCCGCCATGGGATTCTGCTTTTGTTGTGGGGA CAACTGCTTTTTTGAGCAGCCGGCAGTAATTTCATATTGACTGGGACCTGTATATTTACTCTGAGATTTT ACCATTTGCATTTTTTGTTGTTTTGTTTTGTTTTGTTTTGAAACAAGGTCTCACATAGTGCACACTAGCC TTGCTCTCACAATATAACTGAGGTTGACTTCGAACTTGTAAGCCTTCTGCCTACACTTTGTGAGTGTTGG GCTTACAGGCATGTGCCACCACACATGGCTTTATGCAGTGATGGGGATCAAAGCCAGGGAAAGATGCGTG TGAGGGAAACCCGTTTACTAACTGAGCTACAGCCCTAGCCCCTTGCAAATATTTTTAGGAAGTAGAACCA CATTGAGCTTTTTACAAAGCAGAATAAGAGGCTAGGGAGATGGCTAACTGGGTAAAGTGCTTATCGCACA TACACACAAGAAGCTGAATTTGGATCTAGCACATAATAACAAGGCATAGTGGTACCTGCCTGTTCTTGTG CTGAGACGTGTATGCAGTGGGATCCTGAGGGCTTTCCTGGACAGTCAATTTAGCTAATAGTGGTTTAATG AGAGACCCTATCTCAAAAAAAGTTGAAAAATGATAAGACACCTGATAGCGACATCTGTCCTCCACAAGTA TAATCACACACACATACTACACACATGCAATGCAGACAAAGTTATAGAATGATTACAGGGTTGTTGGGCT TTCTAGAATTTGACACTTCTAGAATGTCCTTTCATGATGGGGTTATCTCTGGGGCTAGAGAGATGGCTCA GTGTTCACAAGCATATAATGCTCTTGCAGAGGCCCTGAGTTTGATTGTTAGTATCCATGTCAAGTTGCTC ATTACTGCCTATGACTCTAGTTCCAGGGTATCTGATACCCTTGGCTGGTCTCCATGGACATGGTACTTAT ATTCACACACATGCAAATACACATAGTTTAAAAATAACTTTTAAAAAGCTTAAAGAGAAAATGTGGTTAT CTCTAGGGACAATAATGCCATTCATCTCTTACCTGTAGAAGGAGAATCAGTCATTAGAGATCCCCAAAAG GGTTAAAAACTCGGAAAGGAAGGACTGAATACTTAGATTGTCCTGTGTATTCTGGAGGAAGGAAGCACCA GTGGTCCTATAGTAATCCAGTATGTGTTATAATAAAGTGAATGTTGAAATTAAAAAAAAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:5716144 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5716144 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19771 same embryo
	EMAGE:19767 same embryo
	EMAGE:19769 same embryo
	EMAGE:19768 same embryo
	EMAGE:19770 same embryo
	EurExpress:euxassay_006284 same experiment
	MGI:4828929 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source