EMAGE mouse gene expression spatial database

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EMAGE:20152

Lig1 ligase I, DNA, ATP-dependent ( MGI:101789)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012425. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012425_01	euxassay_012425_02	euxassay_012425_03	euxassay_012425_04

euxassay_012425_05	euxassay_012425_06	euxassay_012425_07	euxassay_012425_08	euxassay_012425_09

euxassay_012425_10	euxassay_012425_11	euxassay_012425_12	euxassay_012425_13	euxassay_012425_14

euxassay_012425_15	euxassay_012425_16	euxassay_012425_17	euxassay_012425_18	euxassay_012425_19

euxassay_012425_20	euxassay_012425_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20152_wholemount_strong.wlz

20152_wholemount_moderate.wlz

20152_wholemount_weak.wlz

20152_wholemount_possible.wlz

20152_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20152_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 10 11 12 13 14

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 13 14 weak expression: see section 10 11 12

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12 13 14

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 08 09 13 14

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 moderate expression: see section 03 04 05

metencephalon rest of alar plate marginal layer

moderate

regional

moderate expression: see section 05 06 09 17 18 19 20 weak expression: see section 07 08 10 11 12 13 15 16

midbrain ventricular layer

strong

regional

strong expression: see section 11 15 16 17 moderate expression: see section 10 12 13 14 18 weak expression: see section 09

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31302
Entity Detected:	Lig1, ligase I, DNA, ATP-dependent ( MGI:101789)
Sequence:	sense strand is shown >T31302 CCCTGTCCCCTCCAGACTTGCTCCCTGTTCTCTATCTCAGCCTCAACCGCCTCGGGCCACCTCAGCAGGG ACTAGAGCTGGGTGTTGGTGATGGTGTCCTCCTCAAGGCCGTTGCCCAGGCCACAGGTCGTCAGCTGGAG TCCATCCGGGCTGAGGTAGCTGAGAAGGGGGATGTGGGGCTGGTGGCCGAGAACAGCCGCAGCACTCAGA GACTCATGCTGCCTCCACCTCCACTTACTATTTCTGGAGTCTTCACCAAATTCTGTGACATTGCCCGGCT CACTGGCAGTGCTTCCATGGCCAAGAAGATGGACATTATCAAGGGCCTTTTTGTTGCCTGCCGCCACTCA GAAGCCAGGTACATTGCCAGGTCCCTAAGCGGGCGGCTGCGCCTTGGCCTAGCTGAGCAGTCGGTACTGG CTGCTCTTGCCCAGGCTGTGAGCCTCACACCCCCTGGCCAAGAATTTCCCACGGCGGTGGTGGATGCTGG GAAGGGCAAGACAGCAGAGGCCAGAAAGATGTGGTTGGAAGAACAAGGCATGATCTTGAAGCAGACCTTC TGTGAGGTTCCTGACCTGGATCGAATCATCCCTGTGCTGCTGGAACATGGCCTGGAACGTCTCCCAGAGC ACTGCAAGCTGAGCCCAGGGGTCCCTCTTAAACCAATGCTGGCTCATCCCACTCGGGGTGTCAGTGAGGT CCTAAAACGCTTTGAGGAGGTCGACTTCACCTGTGAATATAAATACGATGGGCAGCGAGCACAGATACAT GTTCTGGAAGGTGGAGAAGTGAAGATCTTCAGCAGGAACCAGGAAGACAACACAGGAAAGTATCCTGACA TTATCAGCCGCATCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4911261), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 20085. Forward Primer - name:020085_F_IRAV51-54_C22_Lig1, sequence:CCCTGTCCCCTCCAGACT; Reverse Primer - name:020085_R_SP6_IRAV51-54_C22_Lig1, sequence:GGGATGCGGCTGATAATG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20149 same embryo
	EMAGE:20153 same embryo
	EMAGE:20151 same embryo
	EMAGE:20154 same embryo
	EMAGE:20150 same embryo
	EurExpress:euxassay_012425 same experiment
	MGI:4825923 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source