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EMAGE:20153

Ctu2 cytosolic thiouridylase subunit 2 homolog (S. pombe) ( MGI:1914215)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20153 EMAGE:20153 EMAGE:20153 EMAGE:20153 EMAGE:20153
"Pseudo-wholemount" of euxassay_012443. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012443_01 euxassay_012443_02 euxassay_012443_03 euxassay_012443_04
EMAGE:20153 EMAGE:20153 EMAGE:20153 EMAGE:20153 EMAGE:20153
euxassay_012443_05 euxassay_012443_06 euxassay_012443_07 euxassay_012443_08 euxassay_012443_09
EMAGE:20153 EMAGE:20153 EMAGE:20153 EMAGE:20153 EMAGE:20153
euxassay_012443_10 euxassay_012443_11 euxassay_012443_12 euxassay_012443_13 euxassay_012443_14
EMAGE:20153 EMAGE:20153 EMAGE:20153 EMAGE:20153 EMAGE:20153
euxassay_012443_15 euxassay_012443_16 euxassay_012443_17 euxassay_012443_18 euxassay_012443_19
EMAGE:20153
euxassay_012443_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20153Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20153_wholemount_strong.wlz
20153_wholemount_moderate.wlz
20153_wholemount_weak.wlz
20153_wholemount_possible.wlz
20153_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20153_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31329
Entity Detected:Ctu2, cytosolic thiouridylase subunit 2 homolog (S. pombe) ( MGI:1914215)
Sequence:sense strand is shown

>T31329
CCCCCATCAGTGCTATGCTGCACTTCCCAGGAGTCAGCAGGGACAGAGGAGGCCTACAAGGCTGCCGTGG
ACCGCTTCCTGCAGCAGCAGCAGCAGCAGCAGCAGCGTGTGCTGGGAGCTGAGGCCGGTGCCAGCCCAGC
TCAGGGAGAGGCTCGGCTGCATCCATCCCACGGCCGGGAACCCTCAGGCACGGCCGGGTACCCCACAGCT
GCTCAGACAGAGGCCTTGTCTAGGTTGTTCAGCTCCATAAAGACACTGACAGCCAAAGAGGAGCTTCTGC
AGACCCTGAGGACCCATCTGATTGTGCACATAGCCCGAGTCCATGGCTATTGCAAGGTAATGACAGGGGA
GACCTGCACCCGCCTGGCCATCAAGCTTATGACCAACCTGGCCCTGGGGAGAGGCGCCTTCCTCGCCTGG
GACACGGGCTTCTCAGATGAGCGACACGGGGATGTGGTGTTGGTGAGGCCCATGCGGGACCACACGCTGA
AGGAAGTGGCCTTCTACAACCACCTGTTTAGAGTCCCCTCAGTCTTCACACCAGCCATCGATACCAAGGC
TCCAGAAAAGGCTAGCATCCACCGGCTGATGGAGGCCTTTATCCTGAGGCTACAGACTCTGTTCCCGTCT
ACAGTCAGCACCGTGTACAGGACGAGTGAGAAGCTGGTCAAAGCTCCCCGGGAGGGTTGTGCCGCTGGCC
CGTCAGGCCCCAGCTGCCTCCTGTGCATGTGTGCCCTGGACATTGACACTGCTGACAGTGCCACGGCCTT
TGGGGCTCAGTCCTCCTCACATCTCTCCCAGATGCCGAGTGCTGAGGCTGGGATGCCTACCCAGCCCTGC
TGTGCTGCAGGGGAGGGTCAGGCCCAGAGCTGCCATAGGGAAGTTGGCAAGAGGGGGGATGCACGGGCCT
GCATCACAGAGCAGTTATGTTATAGCTGCCGAGTGAACATGAAGGACCTGCCCTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3156706), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 21170. Forward Primer - name:021170_F_IRAV51-54_I10_2310061F22Rik, sequence:CCCCCATCAGTGCTATGC; Reverse Primer - name:021170_R_SP6_IRAV51-54_I10_2310061F22Rik, sequence:GGAGGGCAGGTCCTTCAT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20149 same embryo
 EMAGE:20152 same embryo
 EMAGE:20151 same embryo
 EMAGE:20154 same embryo
 EMAGE:20150 same embryo
 EurExpress:euxassay_012443 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS