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EMAGE:20178

B230354K17Rik RIKEN cDNA B230354K17 gene ( MGI:2443272)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20178 EMAGE:20178 EMAGE:20178 EMAGE:20178 EMAGE:20178
"Pseudo-wholemount" of euxassay_012447. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012447_01 euxassay_012447_02 euxassay_012447_03 euxassay_012447_04
EMAGE:20178 EMAGE:20178 EMAGE:20178 EMAGE:20178 EMAGE:20178
euxassay_012447_05 euxassay_012447_06 euxassay_012447_07 euxassay_012447_08 euxassay_012447_09
EMAGE:20178 EMAGE:20178 EMAGE:20178 EMAGE:20178 EMAGE:20178
euxassay_012447_10 euxassay_012447_11 euxassay_012447_12 euxassay_012447_13 euxassay_012447_14
EMAGE:20178 EMAGE:20178 EMAGE:20178 EMAGE:20178 EMAGE:20178
euxassay_012447_15 euxassay_012447_16 euxassay_012447_17 euxassay_012447_18 euxassay_012447_19
EMAGE:20178 EMAGE:20178 EMAGE:20178 EMAGE:20178 EMAGE:20178
euxassay_012447_20 euxassay_012447_21 euxassay_012447_22 euxassay_012447_23 euxassay_012447_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20178Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20178_wholemount_strong.wlz
20178_wholemount_moderate.wlz
20178_wholemount_weak.wlz
20178_wholemount_possible.wlz
20178_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20178_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
submandibular gland primordium
weak weak
regionalweak expression: see section 08 09 10 11 18 19 20
diencephalon floor plate
weak weak
regionalweak expression: see section 13
telencephalon ventricular layer
weak weak
homogeneousweak expression: see section 02 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22 23 24
medulla oblongata basal plate mantle layer
weak weak
regionalweak expression: see section 10 11 17 18
metencephalon floor plate
weak weak
regionalweak expression: see section 13 14
pons mantle layer
weak weak
regionalweak expression: see section 09 19
midbrain floor plate
weak weak
regionalweak expression: see section 13 14
facial vii ganglion
weak weak
regionalweak expression: see section 06 07
glossopharyngeal ix ganglion
weak weak
regionalweak expression: see section 08 09
trigeminal v ganglion
weak weak
regionalweak expression: see section 04 05 06 07 08 09 18 19 20 21 22 23
vestibulocochlear viii ganglion
weak weak
regionalweak expression: see section 08
ventral grey horn
moderate moderate
single cellmoderate expression: see section 11 weak expression: see section 12 16
dorsal root ganglion
weak weak
regionalweak expression: see section 07 08 09 10 11 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31322
Entity Detected:B230354K17Rik, RIKEN cDNA B230354K17 gene ( MGI:2443272)
Sequence:sense strand is shown

>T31322
GCTGCTGGTTGGTTGCTTTTAGCATCTCTGGATAAACTGATAAAGGATAGGAATGAGCTCCGGGATAAAA
TTAACCAACTGCAGAATATGGTGAAGGACAACAAAGAACTTAATGATAAAATTGACCATCTTCAGATGCT
CATAAACAATCTAAAGGTTTCTAAGTGTACCCTGGAAAAGAATCTTCTCTCCAGCAGCCACAAAGCTCAG
TTTATGGAAAACCAAACTGAAGCTCTCATTATTCGGTTAGCCGAGTTACAGGGAAAATTCAAGTCCCAGC
CTCGGAAAGTGTCAGCAGAACCTGTTCGTAAGGACTACCAGATGCAGTTCCCTTTCAGATGGAAGGCCAG
CAGTATACCTTTACAGTTTTACCTCAAGGATATATAGACTCTCCTGTTCTGTGTCATAACTTAGTTAGAA
GGGAACTTGATTGTCTGACTCTTCCACAAAATATCACATTGGTGCACTATATTGATGATATTATGCTGAC
TGGACCAAGTGAACTGGACGTAGCTACTCATTGGTAACAACAGATATGAATCAGAAGATAGGAAAAATTC
AAGAACTGTCTACAACAGTGAAATCCTTAGGAGTCCAGTGGTGTGGGGAATGCAGAGATATTCCTTGTAA
GGTGAAGAATAAATAATTGCAGTTGACCCCACCCCCCACCAAGAAAGAAGCATAATGTTTAGTGGGCCTA
TTTGGCTTCTGGAAGCATTACAACTCTCACATGGGTTTGTTACTCCAGGCCATATACTAAGTGACTTGGA
AAGCTGTTAGTTTTGGGTGGGGCCTGGGACAGGAGAAGGCTCAACAGATCCAGGCTACTGTGCAGGCTGT
TCTACCACTTGGATGACATGATCCAGCAGACCCAATGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3989671), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 22046. Forward Primer - name:022046_F_IRAV51-54_H14_B230354K17Rik, sequence:GCTGCTGGTTGGTTGCTT; Reverse Primer - name:022046_R_SP6_IRAV51-54_H14_B230354K17Rik, sequence:ACCATTGGGTCTGCTGGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20179 same embryo
 EMAGE:20177 same embryo
 EMAGE:20175 same embryo
 EMAGE:20174 same embryo
 EMAGE:20176 same embryo
 EurExpress:euxassay_012447 same experiment
 MGI:4823369 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS