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EMAGE:20190

Gpbp1 GC-rich promoter binding protein 1 ( MGI:1920524)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20190 EMAGE:20190 EMAGE:20190 EMAGE:20190 EMAGE:20190
"Pseudo-wholemount" of euxassay_012490. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012490_01 euxassay_012490_02 euxassay_012490_03 euxassay_012490_04
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euxassay_012490_05 euxassay_012490_06 euxassay_012490_07 euxassay_012490_08 euxassay_012490_09
EMAGE:20190 EMAGE:20190 EMAGE:20190 EMAGE:20190 EMAGE:20190
euxassay_012490_10 euxassay_012490_11 euxassay_012490_12 euxassay_012490_13 euxassay_012490_14
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euxassay_012490_15 euxassay_012490_16 euxassay_012490_17 euxassay_012490_18 euxassay_012490_19
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euxassay_012490_20 euxassay_012490_21 euxassay_012490_22 euxassay_012490_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20190Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20190_wholemount_strong.wlz
20190_wholemount_moderate.wlz
20190_wholemount_weak.wlz
20190_wholemount_possible.wlz
20190_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20190_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31372
Entity Detected:Gpbp1, GC-rich promoter binding protein 1 ( MGI:1920524)
Sequence:sense strand is shown

>T31372
GCCTACACGCCCAGACACACACATACCCAACCAAAAAAATCTCCCAAGCTCCTCTCTTAGACTATCCCCC
GAATCCTAAATCTAGAACTCCAAGAATGCTGGTCATTAAGAAAGGTAATACAAAAGACTTACAGCTATCT
GGATTCCCAGTAGCAGGAAACCTCCAGTCACAGCCAGTTAAGAATGGAACTGGTCCAAGTGTTTATAAAG
GCTTAGTCCCCAAACCTGCTGTTCCACCTACAAAACCTACACAATGGAAAAGCCAAACTAAAGAAAACAA
AGTCGGGACTTCTTTTTCTCATGAATCTACATATGGTGTTGGCAACTTTAATACTTTTAAGTCAACGGCC
AAGAATATTAGTCCATCAACAAATTCAGTGAAAGAGTGTAATCGTTCAAATTCTTCTTCGCCTGTTGACA
AACTTAATCAGCAGCCTCGTTTAACTAAACTGACACGAATGCGCAGCGATAAAAAGAGTGAATTTTTGAA
AGCATTGAAAAGGGACAGAGTGGAGGAGGAACATGAAGATGAAAGTCATGCTGGCTCAGAGAAGGACGAC
GACTCATTTAATTTGCATAACAGCAATACTACTCACCAAGAAAGAGATATAAACAGAAACTTTGATGAAA
ATGAAATTCCACAGGAGAACGGCAATGCCTCGATGATTTCTCAACAGATCATTCGTTCTTCGACTTTTCC
ACAAACTGATGTTCTTTCCAGTTCACTAGAGGCAGAACACAGATTATTAAAAGAAATGGGCTGGCAGGAA
GACAGTGAAAATGATGAAACATGTGCTCCCTTAACTGAGGATGAAATGAGAGAATTCCAAGTTATTAGTG
AACAGTTACAGAAGAATGGTCTGAGAAAAAATGGTATTTTGAAAAATGGCCTGATCTGTGACTTCAAGTT
TGGACCCTGGAAAAACAGCACTTTCAAACCCACAATTGAGAATGATGACACAGAGACAAGTAGCAGCGAC
ACGTCGGATGATGACG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4501399), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 20555. Forward Primer - name:020555_F_IRAV55-58_E02_1700034P14Rik, sequence:GCCTACACGCCCAGACAC; Reverse Primer - name:020555_R_SP6_IRAV55-58_E02_1700034P14Rik, sequence:TCGTCATCATCCGACGTG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20187 same embryo
 EMAGE:20188 same embryo
 EMAGE:20186 same embryo
 EMAGE:20189 same embryo
 EurExpress:euxassay_012490 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS