EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:20438

Ugt1a7c UDP glucuronosyltransferase 1 family, polypeptide A7C ( MGI:3032636)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013792. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013792_01	euxassay_013792_02	euxassay_013792_03	euxassay_013792_04

euxassay_013792_05	euxassay_013792_06	euxassay_013792_07	euxassay_013792_08	euxassay_013792_09

euxassay_013792_10	euxassay_013792_11	euxassay_013792_12	euxassay_013792_13	euxassay_013792_14

euxassay_013792_15	euxassay_013792_16	euxassay_013792_17	euxassay_013792_18	euxassay_013792_19

euxassay_013792_20	euxassay_013792_21	euxassay_013792_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20438_wholemount_strong.wlz

20438_wholemount_moderate.wlz

20438_wholemount_weak.wlz

20438_wholemount_possible.wlz

20438_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20438_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 06 07 08 09 16 17 18 moderate expression: see section 02 03 04 05 10 11 12 13 14 15 19 20

choroid invagination

strong

regional

strong expression: see section 06 07 08 09 16 17 18 moderate expression: see section 05 10 15

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 06 07 08 09 16 17 18 moderate expression: see section 02 03 04 05 10 11 12 13 14 15 19 20

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05

kidney calyx

strong

regional

strong expression: see section 14 15 16 moderate expression: see section 06 07 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38079
Entity Detected:	Ugt1a7c, UDP glucuronosyltransferase 1 family, polypeptide A7C ( MGI:3032636)
Sequence:	sense strand is shown >T38079 TTCTCCAGACCCCAGTGACTATGACAGACCTCTTCAGCCCAGTGTCCATTTGGTTGTTACGCACAGACTT TGTCTTGGAGTTCCCCAGACCTGTGATGCCCAATGTGATCTACATTGGTGGAATCAACTGCCACCAGGGA AAGCCACTTTCCAAGGAATTTGAAGCCTATGTCAACGCCTCTGGGGAGCATGGCATCGTTGTTTTCTCTT TGGGATCCATGGTCTCAGAGATTCCGGAGAAGAAAGCCATGGAAATTGCTGAGGCTTTGGGCAGAATTCC TCAGACGGTCCTGTGGCGCTACACCGGAACTAGACCATCGAATCTTGCAAAGAACACAATTCTTGTCAAA TGGCTACCCCAAAATGATCTGCTTGGTCATCCAAAGACTCGGGCATTCATCACACACTCTGGCTCCCATG GTATTTATGAAGGAATATGCAATGGAGTTCCGATGGTGATGATGCCCCTATTTGGCGATCAGATGGACAA TGCCAAGCGCATGGAAACTCGGGGAGCTGGGGTGACCCTGAATGTCCTTGAAATGACTGCTGATGATTTG GAAAATGCCCTTAAAACTGTCATCAACAACAAGAGCTACAAGGAGAACATCATGCGCCTCTCCAGCCTTC ACAAGGACCGTCCTATAGAGCCTCTGGACCTGGCTGTGTTCTGGGTGGAATACGTGATGAGGCACAAGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 102109. Forward Primer - name:102109_F_cDNA_Ugt1a10, sequence:TTCTCCAGACCCCAGTGACTAT; Reverse Primer - name:102109_N_SP6_cDNA_Ugt1a10, sequence:CCTTGTGCCTCATCACGTATT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20435 same embryo
	EMAGE:20437 same embryo
	EMAGE:20436 same embryo
	EMAGE:20439 same embryo
	EMAGE:20434 same embryo
	EurExpress:euxassay_013792 same experiment
	MGI:4829086 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source