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EMAGE:20516

Ssu72 Ssu72 RNA polymerase II CTD phosphatase homolog (yeast) ( MGI:1916241)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20516 EMAGE:20516 EMAGE:20516 EMAGE:20516 EMAGE:20516
"Pseudo-wholemount" of euxassay_012786. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012786_01 euxassay_012786_02 euxassay_012786_03 euxassay_012786_04
EMAGE:20516 EMAGE:20516 EMAGE:20516 EMAGE:20516 EMAGE:20516
euxassay_012786_05 euxassay_012786_06 euxassay_012786_07 euxassay_012786_08 euxassay_012786_09
EMAGE:20516 EMAGE:20516 EMAGE:20516 EMAGE:20516 EMAGE:20516
euxassay_012786_10 euxassay_012786_11 euxassay_012786_12 euxassay_012786_13 euxassay_012786_14
EMAGE:20516 EMAGE:20516 EMAGE:20516 EMAGE:20516 EMAGE:20516
euxassay_012786_15 euxassay_012786_16 euxassay_012786_17 euxassay_012786_18 euxassay_012786_19
EMAGE:20516 EMAGE:20516
euxassay_012786_20 euxassay_012786_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20516Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20516_wholemount_strong.wlz
20516_wholemount_moderate.wlz
20516_wholemount_weak.wlz
20516_wholemount_possible.wlz
20516_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20516_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38259
Entity Detected:Ssu72, Ssu72 RNA polymerase II CTD phosphatase homolog (yeast) ( MGI:1916241)
Sequence:sense strand is shown

>T38259
GTAGCGAGAGCAATGGGTAGTTCGGTCGGTGCCATGCCGTCATCGCCGCTGCGGGTGGCGGTGGTGTGCT
CGAGTAACCAGAATCGGAGCATGGAAGCACACAACATCCTCAGCAAGCGAGGTTTCAGTGTCCGCTCCTT
TGGAACAGGAACTCATGTGAAGCTCCCAGGACCAGCACCTGACAAGCCCAATGTTTATGACTTCAAACCC
ACATATGACCAGATGTACAATGACCTCCTCAGGAAAGACAAAGAACTCTATACACAAAATGGAATTTTAC
ATATGTTGGACAGAAATAAAAGGATCAAGCCCCGTCCAGAAAGGTTCCAGAACTGCACGGACCTGTTTGA
CTTGATCCTCACTTGTGAAGAGAGAGTCTATGACCAGGTTGTAGAAGATCTGAACTCCAGAGAACAGGAG
ACCTGCCAGCCAGTGCATGTGGTCAATGTGGACATCCAGGACAACCATGAAGAGGCCACTCTGGGAGCAT
TCCTTATCTGCGAGCTCTGCCAGTGTATCCAGCACACTGAGGACATGGAGAATGAAATCGACGAGCTGCT
GCAGGAGTTCGAGGAGAAGAGTGGCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 142000. Forward Primer - name:142000_F_cDNA_1500011L16Rik, sequence:GTAGCGAGAGCAATGGGTAGTT; Reverse Primer - name:142000_N_SP6_cDNA_1500011L16Rik, sequence:GGCCACTCTTCTCCTCGAACT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20515 same embryo
 EMAGE:20519 same embryo
 EMAGE:20518 same embryo
 EMAGE:20517 same embryo
 EurExpress:euxassay_012786 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS