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EMAGE:20517

1700019D03Rik RIKEN cDNA 1700019D03 gene ( MGI:1914330)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20517
euxassay_013849_01

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20517Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20517_wholemount_possible.wlz
20517_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20517_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38261
Entity Detected:1700019D03Rik, RIKEN cDNA 1700019D03 gene ( MGI:1914330)
Sequence:sense strand is shown

>T38261
CTACTCCAGGGGAGACGTTGTCTCTGCTGCCCGGCCATGCCGCACCTTTTCAGGCTTCGGCTCCCTTCCT
GAGGCCCCGCCCTGGGGGGCAGCACCCACCCTGGGAGCCCGGGCTGCGGCCAGGGGCATGAGAACAGCCA
AGCGCCCGCGCCTCCTCGGCCGAGGCCGCGCAGACTTGCGATGGCGGTGCAGCTGGCCCGCTGCAGTGTA
GGGTAAACACAAGACGTGAAGAGTAGTGAAAGGGACTAGTGCTTGCCAAAGGGGCCTGAGAGGGAAGCCT
GGTCAGCACCGTGTGGACAGCCCTGCACTGTTTTCCAAAGATGCTCTTGAAGTAGTCCTCATTAACACAG
CTTGCCTTCACCTGCCAAAGCTCTTCATCATGGGCTGCATGAAGTCAAAGGAAACGTTCCCATTTCCTAC
CACATTGGACATTGACAAGCTGCATGAGAGTGAGGAGGCCTTTATTCCAGATGACAGCAGTCAATATAGG
ACACCTTCTCCAGGTGAACAACAGCAAGTCCAGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 142380. Forward Primer - name:142380_F_cDNA_1700019D03Rik, sequence:CTACTCCAGGGGAGACGTTGT; Reverse Primer - name:142380_N_SP6_cDNA_1700019D03Rik, sequence:CCTGGACTTGCTGTTGTTCAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20515 same embryo
 EMAGE:20516 same embryo
 EMAGE:20519 same embryo
 EMAGE:20518 same embryo
 EurExpress:euxassay_013849 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS