EMAGE mouse gene expression spatial database

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EMAGE:20548

Lysmd2 LysM, putative peptidoglycan-binding, domain containing 2 ( MGI:1917332)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012778. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012778_01	euxassay_012778_02	euxassay_012778_03	euxassay_012778_04

euxassay_012778_05	euxassay_012778_06	euxassay_012778_07	euxassay_012778_08	euxassay_012778_09

euxassay_012778_10	euxassay_012778_11	euxassay_012778_12	euxassay_012778_13	euxassay_012778_14

euxassay_012778_15	euxassay_012778_16	euxassay_012778_17	euxassay_012778_18	euxassay_012778_19

euxassay_012778_20	euxassay_012778_21	euxassay_012778_22	euxassay_012778_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20548_wholemount_strong.wlz

20548_wholemount_moderate.wlz

20548_wholemount_weak.wlz

20548_wholemount_possible.wlz

20548_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20548_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

bladder

weak

regional

weak expression: see section 11 12 13 14

genital tubercle of male

moderate

regional

moderate expression: see section 11 12 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38265
Entity Detected:	Lysmd2, LysM, putative peptidoglycan-binding, domain containing 2 ( MGI:1917332)
Sequence:	sense strand is shown >T38265 GAGTCACGATGGAACAGATCAAAAGGGCCAACAAACTGTTTAACAATGAACGTATATTTCTGAAGAAGAC GCTGAGCATCCCAATCCTGTCGGAGAAGCCCTTGCTGTTTAATGGACTTAACTCCATTGATTCTCCGGAA AGTGAAACTGTCGACAGCAGCCTTTGTCAGGAAGAGGATCCCGTAGTGTCTGAGGAAGAACTGCCCCCTC CCAGTCCTCAGGACCCGGAGCCCAAGCCTGCGCAACTCGAGGAAGTGTCTGCCAGAGATTTCCTGCACAG ACTGGACTTACAGATTAAACTCTCAACACAGGCAGCCAGGGAACTCAAAGAAGAGAGCAGGGATGAAGAG AGCCCCTATGCTGCCTCTCTTTATCATAGTTAGTGACTGACCAGCGAACTGATCCAGATCACAGAATGGC TGGACCCTAAGAATCCCGCCGGCTCTGGTTCAGGGGCAGCGCTGCCCCTGGACTCCCGTAGCGCACCTTC GAGCTGCAGTTAAGTAGCCCTACTGCAGCTTGCCTGAAGTGATCAGCTCCCGGCTCTCTATAGTTAAGTC TTATTACGGCATGATAGCAAAACTGTA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 142035. Forward Primer - name:142035_F_cDNA_2210402C18Rik, sequence:GAGTCACGATGGAACAGATCAA; Reverse Primer - name:142035_N_SP6_cDNA_2210402C18Rik, sequence:TACAGTTTTGCTATCATGCCG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20546 same embryo
	EMAGE:20549 same embryo
	EMAGE:20550 same embryo
	EMAGE:20551 same embryo
	EMAGE:20547 same embryo
	EurExpress:euxassay_012778 same experiment
	MGI:4826041 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source