EMAGE mouse gene expression spatial database

EMAGE:20747

Olfr2 olfactory receptor 2 ( MGI:97432)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013943. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013943_01	euxassay_013943_02	euxassay_013943_03	euxassay_013943_04

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euxassay_013943_10	euxassay_013943_11	euxassay_013943_12	euxassay_013943_13	euxassay_013943_14

euxassay_013943_15	euxassay_013943_16	euxassay_013943_17	euxassay_013943_18	euxassay_013943_19

euxassay_013943_20	euxassay_013943_21	euxassay_013943_22	euxassay_013943_23	euxassay_013943_24

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Expression pattern clarity:
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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

strong

single cell

strong expression: see section 09 10 14 17

vomeronasal organ

strong

single cell

strong expression: see section 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39209
Entity Detected:	Olfr2, olfactory receptor 2 ( MGI:97432)
Sequence:	sense strand is shown >T39209 CTCTCTGTCACTGGGGCTTCCTATATGGCCATCACAGGTGCAGTGATGCGCATCCCCTCAGCTGCTGGCC GCCATAAGGCCTTTTCAACCTGTGCCTCCCACCTCACTGTTGTGATTATCTTCTATGCAGCCAGTATTTT CATCTATGCCAGGCCTAAGGCACTCTCAGCTTTTGACACCAACAAGCTGGTCTCTGTACTCTACGCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 279079. Forward Primer - name:279079_F_cDNA_Olfr2, sequence:CTCTCTGTCACTGGGGCTTC; Reverse Primer - name:279079_N_SP6_cDNA_Olfr2, sequence:AGCGTAGAGTACAGAGACCAGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20749 same embryo
	EMAGE:20748 same embryo
	EMAGE:20746 same embryo
	EurExpress:euxassay_013943 same experiment
	MGI:4826905 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source