EMAGE mouse gene expression spatial database

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EMAGE:21139

Lpar6 lysophosphatidic acid receptor 6 ( MGI:1914418)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005586. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005586_01	euxassay_005586_02	euxassay_005586_03	euxassay_005586_04

euxassay_005586_05	euxassay_005586_06	euxassay_005586_07	euxassay_005586_08	euxassay_005586_09

euxassay_005586_10	euxassay_005586_11	euxassay_005586_12	euxassay_005586_13	euxassay_005586_14

euxassay_005586_15	euxassay_005586_16	euxassay_005586_17	euxassay_005586_18	euxassay_005586_19

euxassay_005586_20	euxassay_005586_21	euxassay_005586_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21139_wholemount_strong.wlz

21139_wholemount_moderate.wlz

21139_wholemount_weak.wlz

21139_wholemount_possible.wlz

21139_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21139_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5016
Entity Detected:	Lpar6, lysophosphatidic acid receptor 6 ( MGI:1914418)
Sequence:	sense strand is shown >T5016 CACAATACAGCTGGCAACCTCCGAAAGGCCTCTCCATTCAGCAAGCGCGAACATGCTTAGGAATTTATCT GGGATCCCTTAAACGACTGCCTATCGCCGTCCGGAATCAATGTAGAAATACAAAGTTTGAGAATAAAAAG AAGGAAGAAGTACCCGAGGACGACGGGCGGACGGACGCACGGCGAGTGTTTGTGACTGAAGTAAAGCTGG TTTGGACCCTGGCGGCTGAAGCACAAGTTTCCACGCGGACTGGTCTGGTCCGACTTGGAACAGTTTTTCC TTACACTTTCAGCTTTATGGGTTGGCTTCCTTGACTGCATTTTCTGTCAGTTAACTAAACTCCAGACTCA TGGATTTTCTCGACCAGAAAATCAGACTATTTTCCTGAATAATCTACTAGAAACTTTTACGGAACACATT TCATGTTTCCTTTGAAGAGTTAAGAGAAGAAAGTATTTGTAAGAACAGGAAAAGAAACAAATACTTTGCA AATAAACTGGCTGCTGCTGTGACCACATCTGAATAGCAAAGGCGATCGATCAAGCGCTGCGGACAAAAGG CCTCCTGTAAGCTGCACTGCCTGACAATGGTAAGCTCCAATGGCTCCCAGTGCCCTTATGACGACTCCTT TAAGTACACTCTGTACGGGTGCATGTTCAGCATGGTCTTCGTGCTTGGGCTGATATCCAACTGTGTTGCG ATATACATTTTCATCTGTGCCCTCAAAGTGAGAAATGAAACTACAACGTACATGATTAACCTGGCAATGT CAGATTTACTTTTCGTCTTTACTTTGCCATTTCGGATTTTTTACTTTGCAACACGGAATTGGCCATTTGG AGATCTACTCTGTAAGATTTCAGTAATGCTGTTTTACACCAATATGTATGGAAGCATTCTGTTCTTAACC TGTATCAGTGTAGATCGATTTCTGGCAATTGTCTACCCATTTAAGTCAAAGACTTTAAGAACGAAACGAA ATGCAAAGATCGTTTGCATTGCTGTGTGGTTCACAGTGATGGGAGGAAGTGCGCCTGCAGTTTTCTTTCA GTCGACCCACTCTCAGGGGAACAATACCTCAGAAGCCTGCTTTGAGAACTTTCCAGCGGCCACATGGAAA ACTTATCTCTCCAGGATTGTGATTTTCATTGAAATAGTGGGCTTTTTTATCCCTCTCATTTTGAACGTAA CTTGTTCTAGTATGGTGCTAAGAACTTTAAATAAACCTGTTACATTAAGTAGAAGCAAAATGAACAAAAC TAAGGTTTTAAAAATGATTTTTGTCCACTTGGTCATCTTCTGTTTCTGTTTTGTGCCCTACAACATCAAC CTCATTTTGTACTCGCTCATGAGGACACAGACCTTTGTTAACTGCTCTGTGGTGGCGGCAGTGAGGACCA TGTACCCGATCACTCTCTGCATCGCTGTTTCCAACTGCTGCTTTGACCCTATTGTTTACTACTTCACCTC AGACACAATTCAGAACTCAATAAAAATGAAAAACTGGTCGGTTAGAAGAAGTGACTCCAGGTTCTCTGAA GTTCAGGGCACTGAGAATTTTATCCAACACAACCTACAGACCTTAAAAAATAAGATATTTGATAATGAAT CTGCAATATAAGCTGCCTGACTAAGCCACTGGGACTGCTCCGTGTTCAACTGTGAAAACTGTGTTCTTGG GAACTATCTCTCCGGCTCCAACAGAAAATATTTTTAAAGGAAGTTTGTGTCTGATGTGTTAAACATTAAA ATATATTCTATTCTTGTATGCACGCCATTTTACTTTCTTGAACCACTTTAACGTGTTTTTTCCTCATTAA AAAAAAAAAACTCCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5251236 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5251236 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005586 same experiment
	EMAGE:21136 same embryo
	EMAGE:21137 same embryo
	EMAGE:21138 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source