EMAGE mouse gene expression spatial database

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EMAGE:21142

Fam134b family with sequence similarity 134, member B ( MGI:1913520)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004023. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004023_01	euxassay_004023_02	euxassay_004023_03	euxassay_004023_04

euxassay_004023_05	euxassay_004023_06	euxassay_004023_07	euxassay_004023_08	euxassay_004023_09

euxassay_004023_10	euxassay_004023_11	euxassay_004023_12	euxassay_004023_13	euxassay_004023_14

euxassay_004023_15	euxassay_004023_16	euxassay_004023_17	euxassay_004023_18	euxassay_004023_19

euxassay_004023_20	euxassay_004023_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21142_wholemount_strong.wlz

21142_wholemount_moderate.wlz

21142_wholemount_weak.wlz

21142_wholemount_possible.wlz

21142_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21142_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 5 6 1

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 9 0 1 2 3 4 5 6 7 8

heart

weak

regional

weak expression: see section 5 6 7 8 9 0 1 2 3 4 5

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4126
Entity Detected:	Fam134b, family with sequence similarity 134, member B ( MGI:1913520)
Sequence:	sense strand is shown >T4126 GGAAAATGCTGGGCGACTGGGCAGCGCTCCTGCCCAGCTGCCTGTCACGGTGCTGGAGTGAGAGAGCCTG TGCTGTGGACAAGACAGCATTCTCTGGCCATCAGCACAAGCAGAGGCGGCTCAAGTTCTAATTCTCAAAG GGCAGAAATGCCTGCAGGCGGCGGCTGTGGACCAGGCAGAAGCTGGGAAGTCATCAATTCCAAGCCAGAT GAAAGAGCCAGACTGAGCCAGTGCATTGCAGAATCATGGATGAATTTCAGCATGTTTCTTCAAGAAATGT CTCTTTTTAAACAGCAGAGTCCTGGCAAGTTTTGCCTCCTGGTCTGCAGTGTGTGCACATTTTTTACAAT CTTGGGAAGTTACATTCCTGGGGTCATACTCAGCTACCTTCTGTTACTGTTTGCTTTTTTGTGTCCACTG TTTAAATGTAATGATATTGGACAAAAAATATACAGCAAAGTCAAGTCCATTCTATTAAAACTAGATTTTG GAATTGGAGAATATATTAACCAGAAGAAACGTGAGAGATCTGAAGCAGATAAAGAAAAAAGTCACAAGGA TGACAGTGAATTAGACTTTTCAGCTCTTTGTCCTAAGATTAGCCTCACAGTTGCTGCCAAAGAGTTATCT GTGTCAGACACAGATGTGTCAGAAGTCTCTTGGACTGACAATGGGACCTTCAACCTTTCAGAGGGGTACA CTCCGCAGACAGACACTTCTGACGATCTTGACCGGCCCAGTGAGGAAGTTTTCTCTCGAGATCTTTCAGA TTTTCCATCTCTGGAAAATGGGACGGGAACAAATGATGAAGATGAATTAAGCCTGGGCTTACCCACAGAG CTCAAGAGAAAGAAGCAGCAGTTGGATAGTGCTCACAGACCAAGCAAAGAACGGCAGTCGGCAGCTGGCC TCTCACTTCCTCTCAAGAGCGACCAAGCCCTTCACTTGATGAGCAACCTGGCTGGGGATGTCATCACAGC CGCCATGACGGCTGCCATCAAAGACCAGCTAGAAGGAGCCCGGCAGGCACTCACTCAGGTGGCGCCCACT GCGGGAGAGGACACAGACACTGAGGAGGGGGATGACTTTGAACTACTTGACCAGGCAGAGCTAGATCAAA TTGAGAGTGAGCTGGGACTAACACAAGACCAAGGGGCAGAAGCCCAGCAAAGTAAGAAATCCTCAGGCTT CCTCTCCAATCTGCTTGGAGGCCATTAACCGGGAATCACCTGGCAGCCAGCGAAGCACAGATAAACCACC CAAAAAGTTCAAACAAGCAGGGGGAAAAAATGGAAAAAAAATGGAATTGTAAGCTTTAATTACTTTAGTT TTTTTTTCTTTTGTTTTGTTTTCTTTTCCCTTCTATAGTGAGTTGGATGTGTATCAGTTGATAATGATAG ACTGACATTTCTGATAGTTATTTTTCTGTAATAAGCATGGAAATGAACTTTATAGTTACATATATACATA CTGTGGTGTCCTAAGTGCTTAGGGGCTGTTTGTAAAGTGAAGAGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4460992 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4460992 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004023 same experiment
	EMAGE:21143 same embryo
	EMAGE:21144 same embryo
	EMAGE:21145 same embryo
	EMAGE:21146 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source