Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:21199

Cdan1 congenital dyserythropoietic anemia, type I (human) ( MGI:1916218)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21199 EMAGE:21199 EMAGE:21199 EMAGE:21199 EMAGE:21199
euxassay_009002_01 euxassay_009002_02 euxassay_009002_03 euxassay_009002_04 euxassay_009002_05
EMAGE:21199 EMAGE:21199 EMAGE:21199 EMAGE:21199 EMAGE:21199
euxassay_009002_06 euxassay_009002_07 euxassay_009002_08 euxassay_009002_09 euxassay_009002_10
EMAGE:21199 EMAGE:21199 EMAGE:21199 EMAGE:21199 EMAGE:21199
euxassay_009002_11 euxassay_009002_12 euxassay_009002_13 euxassay_009002_14 euxassay_009002_15
EMAGE:21199 EMAGE:21199 EMAGE:21199 EMAGE:21199 EMAGE:21199
euxassay_009002_16 euxassay_009002_17 euxassay_009002_18 euxassay_009002_19 euxassay_009002_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21199Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21199_wholemount_strong.wlz
21199_wholemount_moderate.wlz
21199_wholemount_weak.wlz
21199_wholemount_possible.wlz
21199_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21199_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
diencephalon lateral wall mantle layer
strong strong
regionalstrong expression: see section 8 4 5 6 7 moderate expression: see section 6 7 9 0 2 3
olfactory cortex marginal layer
moderate moderate
single cellmoderate expression: see section 1 2 3 5 6
medulla oblongata basal plate mantle layer
moderate moderate
single cellmoderate expression: see section 6 7 8 9 0 2 3 4 5 6
midbrain mantle layer
strong strong
regionalstrong expression: see section 8 3 4 5 6 moderate expression: see section 5 6 7 9 0 2
ventral grey horn
moderate moderate
single cellmoderate expression: see section 7 8 9 0 1 2 3
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36136
Entity Detected:Cdan1, congenital dyserythropoietic anemia, type I (human) ( MGI:1916218)
Sequence:sense strand is shown

>T36136
TGGAGATAGAAGCCTGCTTAGGCCGCCTTAATGAGGCCCAGTGGCCAGGGGACTTCTCAGAAGAATTGTC
AACACTGTTTCGCTTGTTCCTAGCTGAGCCCCATCTGCTAGAACCCCAGCTAAGAGCTTGTGAACTTATG
CAACCAAACCGAGGGACAGTACTGGCCCAAAGCTAGGGCTAGGAAGCAGCCCTCTTGGGCATGTCACCAG
AACCTGCCTCCTGTGCCTCAGGAGGAGCCTCAAGACCCCACTGCACGCCTTGTTGGGGCAAGAGTGTCCA
GGTGTGTAACCGGGCTTGCTGCTCCTAAGTGAAGGAAGTGTGTTGCTTCAAGCATCCAGACACCGGCCCG
CCTCAACACCTGAGATCTAGAGGAAGGAGAACCTGATTCAGAGTCACAGTGGAAAAGCCCAGAGACTCCG
ATCTTGGAACAGAAGGGCTGGCGTTTTTAATCCAGCTAAAAGAAATGCGTGTGCTGATCAATGTATTTCG
AGCCTCAAGTTGCTATCCCAGCATCTCTTAAAACTGTGGTTCTGAGAAATCTTAATAGGCAAAGAACTTC
TGCTTGTGAGAAAGTTGACAAACATGGATTTGGAAGTCTACCTTTTCTTGTGTCCAGAAGCTGGACTATC
ACCCTTATCCCCAAATATTGACAGCCAATAAGGTAGGACTCGCCTAAGTGAAACTT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 76767. Forward Primer - name:076767_F_cDNA_Cdan1, sequence:TGGAGATAGAAGCCTGCTTAGG; Reverse Primer - name:076767_N_SP6_cDNA_Cdan1, sequence:AAGTTTCACTTAGGCGAGTCCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_009002 same experiment
 EMAGE:21198 same embryo
 EMAGE:21200 same embryo
 EMAGE:21201 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS