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EMAGE:21201

Prdm1 PR domain containing 1, with ZNF domain ( MGI:99655)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21201 EMAGE:21201 EMAGE:21201 EMAGE:21201 EMAGE:21201
euxassay_009010_01 euxassay_009010_02 euxassay_009010_03 euxassay_009010_04 euxassay_009010_05
EMAGE:21201 EMAGE:21201 EMAGE:21201 EMAGE:21201 EMAGE:21201
euxassay_009010_06 euxassay_009010_07 euxassay_009010_08 euxassay_009010_09 euxassay_009010_10
EMAGE:21201 EMAGE:21201 EMAGE:21201 EMAGE:21201 EMAGE:21201
euxassay_009010_11 euxassay_009010_12 euxassay_009010_13 euxassay_009010_14 euxassay_009010_15
EMAGE:21201 EMAGE:21201 EMAGE:21201 EMAGE:21201 EMAGE:21201
euxassay_009010_16 euxassay_009010_17 euxassay_009010_18 euxassay_009010_19 euxassay_009010_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21201Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21201_wholemount_strong.wlz
21201_wholemount_moderate.wlz
21201_wholemount_weak.wlz
21201_wholemount_possible.wlz
21201_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21201_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
vibrissa
weak weak
regionalweak expression: see section 4 5 6 7 9 0
hindgut
weak weak
regionalweak expression: see section 0 1 2 3
midgut
weak weak
regionalweak expression: see section 6 7 8 9 0 1 2 3 4 5
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35519
Entity Detected:Prdm1, PR domain containing 1, with ZNF domain ( MGI:99655)
Sequence:sense strand is shown

>T35519
TTGGTTTTCCTCCTCCTAGTCACAATTTTACCAAACAGTGACAGGAAGGCTTTGCCAACCTGTCTCCCAA
TGTCACATGACCATTCTGAGTGGCCATATGACTTTGGCATCCCTGGGTGTTATCTGAAAATGTGAAGAAG
ATAAAAAAGCCGTGTTCAGAAGATCTGTCGTAAAGCACAGATGTTGTGTGTGTGTGTGTGTGGGTTGGGG
GGTTTGAGTCTGGCTGTCATTTTGCTGTTGGCTTGTTTTTGTTTTTTTAATATCAAAATTGCACAAAGCT
GGTGCCCTACCAAGAAGGATTTGATATAGAAAGGCTCAGGCCACACTTAAAATACAAGCAAGCAAAGAGA
ACAGAAAAAAATAAAAGTAAAAACGGGTATTCTTATCATCTTAGGTTAAGCGGGTAATGAACATTCCTGT
CCCCAACGCATCAACTGTATTGTATCTGTAAAACTCAGCTTTTCTCAGTATTTGTGTTTTTGCATTGTAT
AATTAACTTAATTAAAGATGAAAGGGCATTGCAAAAGTGTTCAACAATTACCTCATTGAGTGTATCCAGT
AGAAGTGCAGGAATTAATGTCGTATCTCATGAGTTGCTACCCAGCTGAGCGTGTGTGCTTCCAAATGGTA
GGCTGGGTGGTTCGGTCCTGTATTCTCCTAAGCCCAAAGGTTACCTGTTGGTGTTCAAGGTGTAATAAAG
AATGCTGTATATTTATGAACCTATTTATACCAGTATACCATGTGTATATATGATATATTTATAACCACTT
AAATTGTGAGCCAAGCCATGTAAAAGAACCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 97414. Forward Primer - name:097414_F_cDNA_Prdm1, sequence:TTGGTTTTCCTCCTCCTAGTCA; Reverse Primer - name:097414_N_SP6_cDNA_Prdm1, sequence:AGGTTCTTTTACATGGCTTGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_009010 same experiment
 EMAGE:21198 same embryo
 EMAGE:21199 same embryo
 EMAGE:21200 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS