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EMAGE:21206

Gpa33 glycoprotein A33 (transmembrane) ( MGI:1891703)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21206 EMAGE:21206 EMAGE:21206 EMAGE:21206 EMAGE:21206
euxassay_012120_01 euxassay_012120_02 euxassay_012120_03 euxassay_012120_04 euxassay_012120_05
EMAGE:21206 EMAGE:21206 EMAGE:21206 EMAGE:21206 EMAGE:21206
euxassay_012120_06 euxassay_012120_07 euxassay_012120_08 euxassay_012120_09 euxassay_012120_10
EMAGE:21206 EMAGE:21206 EMAGE:21206 EMAGE:21206 EMAGE:21206
euxassay_012120_11 euxassay_012120_12 euxassay_012120_13 euxassay_012120_14 euxassay_012120_15
EMAGE:21206 EMAGE:21206 EMAGE:21206 EMAGE:21206 EMAGE:21206
euxassay_012120_16 euxassay_012120_17 euxassay_012120_18 euxassay_012120_19 euxassay_012120_20
EMAGE:21206 EMAGE:21206 EMAGE:21206 EMAGE:21206
euxassay_012120_21 euxassay_012120_22 euxassay_012120_23 euxassay_012120_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21206Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21206_wholemount_strong.wlz
21206_wholemount_moderate.wlz
21206_wholemount_weak.wlz
21206_wholemount_possible.wlz
21206_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21206_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
regionalmoderate expression: see section 1 2 3 4 5 6 7 8 9 0 6 7 8 9 0 1 2 3 4
humerus
moderate moderate
regionalmoderate expression: see section 1 2 3 4 5 0 1 2 3 4
fibula
moderate moderate
regionalmoderate expression: see section 1 2
tibia
moderate moderate
regionalmoderate expression: see section 1 2
femur
moderate moderate
regionalmoderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4
otic capsule
moderate moderate
regionalmoderate expression: see section 8 9 5 6
naris
moderate moderate
regionalmoderate expression: see section 0
nose
moderate moderate
regionalmoderate expression: see section 7 8 9 7 8
meckel's cartilage
moderate moderate
regionalmoderate expression: see section 2 3 4 5 6 7 8 9 0 1 2 4 5 6 7 8 9 0 1
axial skeleton
moderate moderate
regionalmoderate expression: see section 7 8 9 0 1 2 3 4 5 6
basioccipital bone
moderate moderate
regionalmoderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 2
basisphenoid bone
moderate moderate
regionalmoderate expression: see section 8 9 0 1 2 3 4 5 6
temporal bone
moderate moderate
regionalmoderate expression: see section 2
temporal bone petrous part
moderate moderate
regionalmoderate expression: see section 1 2 4 9 0 1
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 1 2 6 7 7 8 9 0
scapula
moderate moderate
regionalmoderate expression: see section 2 3 4 5 0 1 2
pelvic girdle skeleton
moderate moderate
regionalmoderate expression: see section 8 9 6
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30727
Entity Detected:Gpa33, glycoprotein A33 (transmembrane) ( MGI:1891703)
Sequence:sense strand is shown

>T30727
TCCTTGTTCCACCCTCCAAGCCAGACTGCAGCATCCAAGGGGAGATGGTGATCGGGAACAACATCCAGCT
GACATGCCACTCTGCAGAGGGCTCTCCGAGCCCACAATACAGCTGGAAGAGTTACAACGCCCAGAACCAG
CAGCGGCCCCTCACCCAGCCAGTCTCAGGCGAGCCATTGTTGCTGAAGAACATCTCCACGGAGACAGCGG
GTTACTACATCTGCACCTCCAGCAATGACGTGGGGATAGAGTCCTGCAACATCACCGTGGCACCCAGACC
TCCCTCCATGAACATTGCGCTGTATGCGGGCATTGCGGGGGGTGTCTTTGTGGCCCTCATCATCATCGGT
GTCATTGTCTACTGCTGCTGCTGCCGGGAAAAGGACGACAAAGATCAAGACAGGGAGGATGCGCGGCCGA
ACCGAGCAGCTTACCAAGTGCCTAAAAAGGAGCAGAAAGAAATTTCCAGAGGGCGGGAAGACGAAGATGA
CCACAGACATGAGGATCGGTGGAGCTCGGGGCGTAGCACTCCAGACCAGCCTTTCCAATGAAGGAGCCTT
CTCCCCAGCCCCATAGTCCTGCTCCTCCCCCCAGGCATTGGTGGAGCACTTCTTTCTGTGTATCTGCCTC
AGGGGCGCATCACTGGCCTGGCTACTGACGTCCTACCTGATGACCACTGAAAGGACCCTTCCTCATTGCT
AAATCTGACTCAGGGTCTGGCCTTCTCCCTAGACCAGACAGCATCCTCTGCCTCTCTTACAGCTGGCAGG
GCCTCCTAGAATCTCGGCACGATGCAGGGCTGCTTCTGTTCCAAGCTCGCCATACAGGCAAGCGCCAGCT
TCAGCACCAGTTTCAGAAGGTGGGAGTCTGCCCTGCCTCACCATGCCCGACACTCAGAGGAGAACCAGTG
ATGGGCCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3593917), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 12974. Forward Primer - name:012974_F_IRAV12-15_L16_Gpa33, sequence:TCCTTGTTCCACCCTCCA; Reverse Primer - name:012974_R_SP6_IRAV12-15_L16_Gpa33, sequence:CAGGCCCATCACTGGTTC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_012120 same experiment
 EMAGE:21207 same embryo
 EMAGE:21208 same embryo
 EMAGE:21209 same embryo
 EMAGE:21210 same embryo
 EMAGE:21211 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS