EMAGE mouse gene expression spatial database

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EMAGE:21209

Nans N-acetylneuraminic acid synthase (sialic acid synthase) ( MGI:2149820)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_012123_01	euxassay_012123_02	euxassay_012123_03	euxassay_012123_04	euxassay_012123_05

euxassay_012123_06	euxassay_012123_07	euxassay_012123_08	euxassay_012123_09	euxassay_012123_10

euxassay_012123_11	euxassay_012123_12	euxassay_012123_13	euxassay_012123_14	euxassay_012123_15

euxassay_012123_16	euxassay_012123_17	euxassay_012123_18	euxassay_012123_19	euxassay_012123_20

euxassay_012123_21	euxassay_012123_22	euxassay_012123_23	euxassay_012123_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21209_wholemount_strong.wlz

21209_wholemount_moderate.wlz

21209_wholemount_weak.wlz

21209_wholemount_possible.wlz

21209_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21209_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 4 5 6 7 8 9 0 1 2

radius

moderate

regional

moderate expression: see section 1

humerus

moderate

regional

moderate expression: see section 1 2 3 4

hindlimb digit 1 phalanx

moderate

regional

moderate expression: see section 5

hindlimb digit 2 metatarsal

moderate

regional

moderate expression: see section 4

hindlimb digit 2 phalanx

moderate

regional

moderate expression: see section 4 5

hindlimb digit 3 metatarsal

moderate

regional

moderate expression: see section 4

hindlimb digit 3 phalanx

moderate

regional

moderate expression: see section 4 5

hindlimb digit 4 phalanx

moderate

regional

moderate expression: see section 5

hindlimb digit 5 phalanx

moderate

regional

moderate expression: see section 5

tarsus

moderate

regional

moderate expression: see section 4

fibula

moderate

regional

moderate expression: see section 1

tibia

moderate

regional

moderate expression: see section 1 2 3

femur

moderate

regional

moderate expression: see section 1 2 3 4 5 6

skin

moderate

regional

moderate expression: see section 5

otic capsule

moderate

regional

moderate expression: see section 7 8 5 6

naris

moderate

regional

moderate expression: see section 0 1 2 3 5 6

nose

moderate

regional

moderate expression: see section 6 7 8 9 7 8

hyoid

moderate

regional

moderate expression: see section 3

meckel's cartilage

moderate

regional

moderate expression: see section 2 3 4 5 6 7 8 9 0 1 2 4 5 6

axial skeleton

moderate

regional

moderate expression: see section 7 8 9 0 1 2 3 4 5 6

basioccipital bone

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3

basisphenoid bone

moderate

regional

moderate expression: see section 8 9 0 1 2 3 4 5 6

temporal bone petrous part

moderate

regional

moderate expression: see section 1 2 3 0 1 2

vault of skull

moderate

regional

moderate expression: see section 5 6 8 9 0 7 8 9 0 1 2 3

orbito-sphenoid

moderate

regional

moderate expression: see section 1 2 3 5 6 7 7 8 9 0 1 2

clavicle

moderate

regional

moderate expression: see section 5 6 7 8 9

scapula

moderate

regional

moderate expression: see section 2 3 4

pelvic girdle skeleton

moderate

regional

moderate expression: see section 8 9 6

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30731
Entity Detected:	Nans, N-acetylneuraminic acid synthase (sialic acid synthase) ( MGI:2149820)
Sequence:	sense strand is shown >T30731 CGCTGGAACTGGAGCTGTGTCCCGGGCGCTGGGTGGGTGGAAAGCACCCGTGCTTCATCATCGCGGAGAT CGGCCAGAACCACCAAGGAGACATAGATGTGGCCAAGCGCATGATCCGCACTGCCAAGGAGTGTGGGGCC GACTGCGCTAAGTTTCAGAAGAGCGAGTTGGAGTTCAAGTTTAACCGGAAGGCCCTGGAGAGACCATATA CTTCGAAGCATTCATGGGGGAAGACGTATGGGGAGCACAAGCGGCATCTGGAATTCAGCCACGACCAGTA CAAGGAGCTGCAGAGCTATGCGCAGGAGATCGGCATCTTCTTCACTGCCTCTGGCATGGACGAGATGGCA GTTGAGTTTCTGCACGAACTGAATGTTCCCTTTTTCAAAGTTGGATCTGGGGACACTAACAATTTTCCCT ACCTGGAAAAGACAGCCAAGAAAGGTCGTCCTATGGTGATCTCCAGCGGGATGCAGTCAATGGACACCAT GAAGCAAGTCTATCAGATCGTGAAGCCGCTGAATCCCAACTTCTGCTTCCTCCAATGCACCAGCGCGTAC CCACTACAGCCCGAGGATGCCAACCTGCGCGTCATCTCGGAATACCAGAAGCTCTTTCCCGACATTCCCA TCGGGTATTCCGGGCACGAGACGGGCATCGCCATATCTGTGGCCGCCGTGGCTCTGGGGGCCAAGGTGTT GGAACGTCACATAACGTTGGACAAGACCTGGAAGGGGAGTGACCACTCAGCCTCGCTGGAGCCTGGGGAG CTGGCAGAGCTGGTGCGGTCTGTGCGCCTGGTGGAGCGGGCCCTGGGCTCCCCAACCAAGCAGCTGCTGC CCTGTGAGATGGCCTGCAATGAGAAGCTCGGCAAGTCTGTGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3154822), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 13394. Forward Primer - name:013394_F_IRAV01-03_A10_Nans, sequence:CGCTGGAACTGGAGCTGT; Reverse Primer - name:013394_R_SP6_IRAV01-03_A10_Nans, sequence:ACCACAGACTTGCCGAGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_012123 same experiment
	EMAGE:21206 same embryo
	EMAGE:21207 same embryo
	EMAGE:21208 same embryo
	EMAGE:21210 same embryo
	EMAGE:21211 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source