EMAGE mouse gene expression spatial database

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EMAGE:21253

Eaf2 ELL associated factor 2 ( MGI:2146616)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002118_01	euxassay_002118_02	euxassay_002118_03	euxassay_002118_04	euxassay_002118_05

euxassay_002118_06	euxassay_002118_07	euxassay_002118_08	euxassay_002118_09	euxassay_002118_10

euxassay_002118_11	euxassay_002118_12	euxassay_002118_13	euxassay_002118_14	euxassay_002118_15

euxassay_002118_16	euxassay_002118_17	euxassay_002118_18	euxassay_002118_19	euxassay_002118_20

euxassay_002118_21	euxassay_002118_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21253_wholemount_strong.wlz

21253_wholemount_moderate.wlz

21253_wholemount_weak.wlz

21253_wholemount_possible.wlz

21253_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21253_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4638
Entity Detected:	Eaf2, ELL associated factor 2 ( MGI:2146616)
Sequence:	sense strand is shown >T4638 GCTGTTCCCACTAAGGACTATGGCCTCGGCTTCTGAGGAGACTTTGGACTAGGGGCGCCAGCTGCTAGTA TTTGCGATTTTGTGAGAACCAACCCGGAGTAGAGCCTGAAACTTGGTCCTTCCGATGGGGGAGAAAGCGA TTTAGTAACGGAACTCCGATTTCAACCTGACGTCACAGGACATCACTCAGGATGTGAGGATGACTTCAAA CCTGCTTCTATTGATACTTCTTGTGAAGGAAATCTTGAGGTTGGCAAAGGTGAACAGGTGACAATAACTC TTCCAAATATAGAAGGTTCAACTCCACCAGTCACAGTTTTCAAAGGTTCCAAGAGACCTTACTTAAAAGA ATGCATTTTGATTATTAACCATGATACTGGGGAATGTCGCCTAGAAAAGCTCAGCAGCAACATCACTGTG AAAAAAACAAGAGTGGAAGGGAGTAGCAGAATCCAGTACAGACTAGAACAACAGCAACAGCAAATGTGGA ATCTGCCTAGGACTTCCAATCTTGTACAGCATTCTCCATCAGAAGAGAAGATGTCTCCAACGTCTCTAAT GGATGATATTGAAAGAGAACTGAAAGCAGAAGCTAGTCTTATGGACCAGATGAGTAGTTGTGATAGTTCA TCAGATTCCAAAAGTTCTTCATCTTCAAGTAGTGAGGATAGTTCTAGTGATTCTGAAGATGATGACCAAT TCTCTCCTTTGGGTCCAAGGAAATACAGCTCGGAGCACCCTAGCATGTCTGCTGGGCCACAGTACAGGAC TTCAGAGGCTGATGCTACTTGTCACCGACTTCAGGACCACAGTACCCTTCTGATGAGTACTTTACGAAGT GACTTGCAGCTGAGTGAGTCAGAAAGCGACAGTGAGGACTGAAGCAGTATCAAGCTACAGAGAAAACATT TGTGAGATGTGTAAGAATCTGTTTTGTATTGAGAATAAATATTCCTATGTTTATGGAAATTGTGCAACTT TTGCTGAAAAAATAAAGTTGGTTCAGAATTTTCAACTATTTGTTGGTATTCTCATGTGATATCTTCATTT AGTCATAACAAAATGTTATCACATTTGGTAGATGAAAAACAGATTCAGATATTAGGGAACTTAACTTGCC CCAGGAATTGCTAACAAATGAGCTGCAGATCCACTATTCCAGAGTCTCTGTCCACTGTTGCCCCAAATTG TGAAATGCTAAAATAATTGATTTATAGTAAGAGTTTATGGTTTTCATTGTAGTTGTGCCTTTCCTATCTG ATATGCCTTAAATTATGACTGAATATACTTTAATTCCTGCCAAATTTACACTTTATATAAAATTGAAGTT AAATAATTATCCTAATTGATGTTTTGTTATCTCTTATAATGAAAGATCCTGACAGAATGTAAAAGGACAC AGAAGCCCTGAAATTGGCAAGATAGATGTCAGTGTTAGCAGAACTAGCTTCACTGATTTTAAAAAATAGA GGTGCACAATGCTCTGGCCACTCCTCGAACCTGTGTGTCTGCCAATGTTTTGACCGTTCCTTGACCCATG TGTGTGCCCACTGATGAAGCCCATTGCCAGGTGTTTGTGATATCGGTTGCTGAGAAAGAGTCAGAAAATC TCCCCAGCAACCACAGCCCAGCCAATCCTGAGTTGCTACACCTGCCCCAGACTCTTTCCTTGTACCCGTC CCATACCCATTTCTTGAGAATAGACATTGTTTAGATCTGGAAATCCCCTACTCTCCCCCTTCTCCTTTCT CCCCTGAGGGCCTATAAAAACTGGGAACTCTTTCCTCTCGAGGTCAACTCCTCTACCCCTGTGTGGGATA TGAGTCGTCCCCAGAGCTCTGGCTTTCCCTGAATAAAGCCTCATGTGGTTTGCAACAAAAAAAAAAAAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5008372 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5008372 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002118 same experiment
	EMAGE:21252 same embryo
	EMAGE:21254 same embryo
	EMAGE:21255 same embryo
	EMAGE:21256 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source