EMAGE mouse gene expression spatial database

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EMAGE:21276

Tlr3 toll-like receptor 3 ( MGI:2156367)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001994. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001994_01	euxassay_001994_02	euxassay_001994_03	euxassay_001994_04

euxassay_001994_05	euxassay_001994_06	euxassay_001994_07	euxassay_001994_08	euxassay_001994_09

euxassay_001994_10	euxassay_001994_11	euxassay_001994_12	euxassay_001994_13	euxassay_001994_14

euxassay_001994_15	euxassay_001994_16	euxassay_001994_17	euxassay_001994_18	euxassay_001994_19

euxassay_001994_20	euxassay_001994_21	euxassay_001994_22	euxassay_001994_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21276_wholemount_strong.wlz

21276_wholemount_moderate.wlz

21276_wholemount_weak.wlz

21276_wholemount_possible.wlz

21276_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21276_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 8 9 0 7 8 9 0

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1153
Entity Detected:	Tlr3, toll-like receptor 3 ( MGI:2156367)
Sequence:	sense strand is shown >T1153 CCTCGAGNCTGTTGGCCTACTGGCATCTACAAAGTTGGGAACGGGGGTCCAACTGGAGAACCTCCAAGAA CTGCTCTTAGCAAAAAATAAAATCCTTGCGTTGCGAAGTGAAGAACTTGAGTTTCTTGGCAATTCTTCTT TACGAAAGTTGGACTTGTCATCAAATCCACTTTAAGAGTTCTCCCCGGGGTGTTTCCAGACAATTGGCAA GTTATTCGCCCTCCTCTTGAACAACGCCCAACTGAACCCCCACCTCACAGAGAAGCTTTGCTGGGAACTT TCAAACACAAGCATCCAGAATCTCTCTCTGGCTAACAACCAGCTGCTGGCCACCAGCGAGAGCACTTTCT CTGGGCTGAAGTGGACAAATCTCACCCAGCTCGATCTTTCCTACAACAACCTCCATGATGTCGGCAACGG TTCCTTCTCCTATCTCCCAAGCCTGAGGTATCTGTCTCTGGAGTACAACAATATACAGCGTCTGTCCCCT CGCTCTTTTTATGGACTCTCCAACCTGAGGTACCTGAGTTTGAAGCGAGCATTTACTAAGCAAAGTGTTT CACTTGCTTCACA
Notes:	The probe template was PCR amplified from IMAGE:2136509 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2136509 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001994 same experiment
	EMAGE:21275 same embryo
	EMAGE:21277 same embryo
	EMAGE:21278 same embryo
	EMAGE:21279 same embryo
	EMAGE:21280 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source