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EMAGE:21292

Abca5 ATP-binding cassette, sub-family A (ABC1), member 5 ( MGI:2386607)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21292 EMAGE:21292 EMAGE:21292 EMAGE:21292 EMAGE:21292
"Pseudo-wholemount" of euxassay_009385. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009385_01 euxassay_009385_02 euxassay_009385_03 euxassay_009385_04
EMAGE:21292 EMAGE:21292 EMAGE:21292 EMAGE:21292 EMAGE:21292
euxassay_009385_05 euxassay_009385_06 euxassay_009385_07 euxassay_009385_08 euxassay_009385_09
EMAGE:21292 EMAGE:21292 EMAGE:21292 EMAGE:21292 EMAGE:21292
euxassay_009385_10 euxassay_009385_11 euxassay_009385_12 euxassay_009385_13 euxassay_009385_14
EMAGE:21292 EMAGE:21292 EMAGE:21292 EMAGE:21292 EMAGE:21292
euxassay_009385_15 euxassay_009385_16 euxassay_009385_17 euxassay_009385_18 euxassay_009385_19
EMAGE:21292 EMAGE:21292 EMAGE:21292
euxassay_009385_20 euxassay_009385_21 euxassay_009385_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21292Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21292_wholemount_strong.wlz
21292_wholemount_moderate.wlz
21292_wholemount_weak.wlz
21292_wholemount_possible.wlz
21292_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21292_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35858
Entity Detected:Abca5, ATP-binding cassette, sub-family A (ABC1), member 5 ( MGI:2386607)
Sequence:sense strand is shown

>T35858
CCTGGGTTGTCTGATTTCTTTCATAAAGGGTTCTTGGAAGAATATGCCAAAAAATGAGAATACCTACAAT
CCCTGGGATAGACTTTTAGTTGCTGTAATCATGCCCTACCTGCAGTGTATACTGTGGATTTTCCTCTTAC
AACACTATGAGAAAATACATGGAGGCAGATCTATAAGAAAGGATCCATTTTTCAGGGCCCTTTCACAAAA
GGCCAAAAATAAGAAGTTTCCAGAACCACCCATCAATGAGGATGAAGATGAAGATGTCAAAGCTGAAAGA
CTGAAGGTTAAAGAGCTGATGGGTTGCCAGTGTTGTGAGGAGAAGCCAGCCATTATGGTATGCAATTTGC
ATAAAGAGTATGATGACAAGAAAGATTTTCTTCATTCAAGAAAAACAACAAAAGTAGCAACAAAATACAT
CTCTTTCTGTGTGAAAAAAGGAGAGATCTTGGGACTGTTGGGTCCAAATGGAGCTGGCAAAAGCACAGTC
ATTAACACTCTGGTTGGGGACGTTGAGCCAACTTCAGGCAAGATCTTTCTAGGAGATTATGGTTCACATT
CAAGTGAAGATGACGAATCCATTAAGTGTATGGGGTACTGCCCCCAGACAAACCCACTGTGGCCAGATCT
CACTCTACAGGAACA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 92794. Forward Primer - name:092794_F_cDNA_Abca5, sequence:CCTGGGTTGTCTGATTTCTTTC; Reverse Primer - name:092794_N_SP6_cDNA_Abca5, sequence:TGTTCCTGTAGAGTGAGATCTGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_009385 same experiment
 EMAGE:21290 same embryo
 EMAGE:21291 same embryo
 EMAGE:21293 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS