EMAGE mouse gene expression spatial database

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EMAGE:21293

Abca9 ATP-binding cassette, sub-family A (ABC1), member 9 ( MGI:2386796)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009386. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009386_01	euxassay_009386_02	euxassay_009386_03	euxassay_009386_04

euxassay_009386_05	euxassay_009386_06	euxassay_009386_07	euxassay_009386_08	euxassay_009386_09

euxassay_009386_10	euxassay_009386_11	euxassay_009386_12	euxassay_009386_13	euxassay_009386_14

euxassay_009386_15	euxassay_009386_16	euxassay_009386_17	euxassay_009386_18	euxassay_009386_19

euxassay_009386_20	euxassay_009386_21	euxassay_009386_22	euxassay_009386_23	euxassay_009386_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21293_wholemount_strong.wlz

21293_wholemount_moderate.wlz

21293_wholemount_weak.wlz

21293_wholemount_possible.wlz

21293_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21293_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 9 0 3 4 5 6 7 8 9 0 1 2 3 weak expression: see section 8

diencephalon meninges

moderate

regional

moderate expression: see section 3 4 5 6 7 8 weak expression: see section 2

telencephalon meninges

moderate

regional

moderate expression: see section 3 4 5 6 7 8 weak expression: see section 2

hindbrain meninges

moderate

regional

moderate expression: see section 3 4 5 6 7 8 weak expression: see section 2

midbrain meninges

moderate

regional

moderate expression: see section 3 4 5 6 7 8 weak expression: see section 2

tail paraxial mesenchyme

strong

regional

strong expression: see section 7 8 9 0 1 2 3 4 5 moderate expression: see section 6 7 8

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35859
Entity Detected:	Abca9, ATP-binding cassette, sub-family A (ABC1), member 9 ( MGI:2386796)
Sequence:	sense strand is shown >T35859 GAGCATCACTGTGCCTTACTTGGTCGGTGTGCAAACAGCTTAGAACACGCAGCCAGACTACGCTCAATCA AGTCACGTTGCACACAGTGTGGAGTTTTAAACTGCATTTACAATAGTATTGATGCTTCTGAGCATGCTAA AGAGACTGATTGATTTTCTTCGCCTGAACTTCAAAACATTAACTTTTTTCTTTCCTCCGTGTCTAGGTAA GGTATAATGTAAACAATATTGATTTTAGGGAGAAGAGGATCAATTGATTTCCCAACAGCACAGTATTTGT TTTTTTGTGTAACTCACCCTGACCTGTCTGTCATTCACTAGTTCTTTACTCACAGTTTGCCCACTCTGAG TTACGGAGAAATGTTTTGTCCACTGTGACTGACTGCGGAAGCAACTTTAAACTGGTGGACGTAAATACTT GCAACCATTAAATAACTGTGTGCATGTTTACATACGAATCTGAAACATTTCTGAAATTTTGATTTGGGAA AAAGTAATGCAGAGGAGGGGTACTCTTAGGTCCAAGGACTGGCTTTGTATGTATTTATTTACCAAGTCTG TGAATGCTACAACCTCTCTACTGCAACATCAGTACAGTTACCTGGACTCTTATCTAGGAGATTTTTTTGT TGTTACTACTAGTATTGTTACTAATTAACCAAACATCTATATGAAATTATAAGTTAATATGAAAAAATTA TATGAAAAAAAATTCCCTTGATGCTAAGATTGTAACTTCATAGTGTATGTGAATATCAACTGCCCTCAAC TTACAAGGAGACTTCTCAAGTAGACTTTGAGCAAAGGCTCTAAGCCTTTAAAGTTAAAAAGAGACATTAA TGCTGGACTTGCTGATGTACCTGCTTTATG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 92789. Forward Primer - name:092789_F_cDNA_Abca9, sequence:GAGCATCACTGTGCCTTACTTG; Reverse Primer - name:092789_N_SP6_cDNA_Abca9, sequence:CATAAAGCAGGTACATCAGCAAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_009386 same experiment
	EMAGE:21290 same embryo
	EMAGE:21291 same embryo
	EMAGE:21292 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source