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EMAGE:21343

Crygs crystallin, gamma S ( MGI:1298216)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21343 EMAGE:21343 EMAGE:21343 EMAGE:21343 EMAGE:21343
"Pseudo-wholemount" of euxassay_000138. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_000138_01 euxassay_000138_02 euxassay_000138_03 euxassay_000138_04
EMAGE:21343 EMAGE:21343 EMAGE:21343 EMAGE:21343 EMAGE:21343
euxassay_000138_05 euxassay_000138_06 euxassay_000138_07 euxassay_000138_08 euxassay_000138_09
EMAGE:21343 EMAGE:21343 EMAGE:21343 EMAGE:21343 EMAGE:21343
euxassay_000138_10 euxassay_000138_11 euxassay_000138_12 euxassay_000138_13 euxassay_000138_14
EMAGE:21343 EMAGE:21343 EMAGE:21343 EMAGE:21343 EMAGE:21343
euxassay_000138_15 euxassay_000138_16 euxassay_000138_17 euxassay_000138_18 euxassay_000138_19
EMAGE:21343 EMAGE:21343 EMAGE:21343 EMAGE:21343 EMAGE:21343
euxassay_000138_20 euxassay_000138_21 euxassay_000138_22 euxassay_000138_23 euxassay_000138_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21343Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21343_wholemount_strong.wlz
21343_wholemount_moderate.wlz
21343_wholemount_weak.wlz
21343_wholemount_possible.wlz
21343_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21343_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1761
Entity Detected:Crygs, crystallin, gamma S ( MGI:1298216)
Sequence:sense strand is shown

>T1761
CAACCTTTGTTCNACAATGTCTAAAACTGGAGGCAAGATTAGTTTCTACGAAGACCGAAATTTTCAAGGC
CGCCGCTATGACTGCGACTGCGACTGCGCAGACTTCCGCTCGTACCTAAGTCGCTGCAACTCCATTAGAG
TAGAGGGAGGCACCTGGGCTGTGTATGAAAGGCCCAACTTCTCCGGGCACATGTACATCTTACCCCAGGG
CGAGTACCCTGAATACCAGCGTTGGATGGGCCTTAATGACCGCCTGGGCTCTTGCCGAGCTGTTCATCTG
TCTAGCGGAGGCCAGGCTAAGATTCAGGTCTTTGAAAAGGGCGACTTCAACGGTCAGATGTACGAAACCA
CGGAAGACTGTCCTTCTATCATGGAGCAGTTTCACCTGCGAGAGATTCATTCCTGTAAGGTGGNAGAAGG
CACCTGGATTTTCTATGAGCTACCCAACTACCGTGGCAGACAGTATCTTCTGG
Notes:The probe template was PCR amplified from IMAGE:483498 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:483498 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_000138 same experiment
 EMAGE:21345 same embryo
 EMAGE:21344 same embryo
 EMAGE:21346 same embryo
 EMAGE:21342 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS