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EMAGE:21462

Fbxl6 F-box and leucine-rich repeat protein 6 ( MGI:1354705)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21462 EMAGE:21462 EMAGE:21462 EMAGE:21462 EMAGE:21462
euxassay_002832_01 euxassay_002832_02 euxassay_002832_03 euxassay_002832_04 euxassay_002832_05
EMAGE:21462 EMAGE:21462 EMAGE:21462 EMAGE:21462 EMAGE:21462
euxassay_002832_06 euxassay_002832_07 euxassay_002832_08 euxassay_002832_09 euxassay_002832_10
EMAGE:21462 EMAGE:21462 EMAGE:21462 EMAGE:21462 EMAGE:21462
euxassay_002832_11 euxassay_002832_12 euxassay_002832_13 euxassay_002832_14 euxassay_002832_15
EMAGE:21462 EMAGE:21462 EMAGE:21462 EMAGE:21462 EMAGE:21462
euxassay_002832_16 euxassay_002832_17 euxassay_002832_18 euxassay_002832_19 euxassay_002832_20
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euxassay_002832_21 euxassay_002832_22 euxassay_002832_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21462Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21462_wholemount_strong.wlz
21462_wholemount_moderate.wlz
21462_wholemount_weak.wlz
21462_wholemount_possible.wlz
21462_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21462_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T359
Entity Detected:Fbxl6, F-box and leucine-rich repeat protein 6 ( MGI:1354705)
Sequence:sense strand is shown

>T359
CGTGGCCTCTAGGCGAGTTAGACGTCGAGTTCGAAGCTCCAAGCGGCCCGATGCGCGGGGCCGCTCGGCG
GAGGACTGGTGGTGGGACCGCCTGGCACCTCGCGGCTCTGGGTACCACCTGCTGCAAGCGGACAGCATGT
TGCTCGTGCTGCCGGACCTGGAGCCTCCCCGCGCTCGTGCGCACCGGCGCGCCCGTCGCCGCGCTCCGAG
GTCACTGGCTCGTGGCCCCACGGCTGTCGCCAAGCCCAGGACCAAACCCCGACCGGAGCCGTCGCTCGAT
CAGGGCCTGGACTCTGGTTGGGGAGACCGTATTCCCTTAGAAGTCCTGGTGCATATTTTCGGGTTGTTGG
TTGCGGCTCATGGGCCCATGCCGTTTCTTGGCAGGGCTGCACGCGTTTGCCGTCACTGGCACGAAGCCAC
CTCCCATCCTTCACTCTGGCATACTGTGACCCTGTCACCCTCGCTG
Notes:The probe template was PCR amplified from IMAGE:3155607 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3155607 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_002832 same experiment
 EMAGE:21460 same embryo
 EMAGE:21464 same embryo
 EMAGE:21459 same embryo
 EMAGE:21463 same embryo
 EMAGE:21461 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS