EMAGE mouse gene expression spatial database

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EMAGE:21481

Gtf2h3 general transcription factor IIH, polypeptide 3 ( MGI:1277143)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002982_01	euxassay_002982_02	euxassay_002982_03	euxassay_002982_04	euxassay_002982_05

euxassay_002982_06	euxassay_002982_07	euxassay_002982_08	euxassay_002982_09	euxassay_002982_10

euxassay_002982_11	euxassay_002982_12	euxassay_002982_13	euxassay_002982_14	euxassay_002982_15

euxassay_002982_16	euxassay_002982_17	euxassay_002982_18	euxassay_002982_19	euxassay_002982_20

euxassay_002982_21	euxassay_002982_22	euxassay_002982_23	euxassay_002982_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21481_wholemount_strong.wlz

21481_wholemount_moderate.wlz

21481_wholemount_weak.wlz

21481_wholemount_possible.wlz

21481_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21481_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 7 8 9 0 7 8 moderate expression: see section 5 6

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4266
Entity Detected:	Gtf2h3, general transcription factor IIH, polypeptide 3 ( MGI:1277143)
Sequence:	sense strand is shown >T4266 CGGAGGCGTAGGGAAGCAATGGCCGCTGACGAAGATGAGTTGAACCTTCTGGTGATCATAGTTGACACCA ACCCGATTTGGTGGGGAAAGCAAGCATTAAAGGAATCTCAGTTTACTTTATCCAAATGCATGGATGCAGT GATGGTGCTGGCGAATTCTCACCTGTTCATGAACCGCTCCAACCAGCTGGCTGTCATCGCCAGTCACATT CAGGAAAGCCGGCTCTTATACCCGGGGAAGAACGGTGGACTTGGAGACTTCTTCGGAGACCCTGGCAATG CGCTCCCCGACTGTAACCCCTCTGGGAGTAAAGATGGGAAATACGAGCTGTTGACAGTTGCAAACGAGGT GATCGCTGAGGAGATCAAGGATCTGATGACCAAGAGTGACATCAAGGGCCAGCATACGGAGACACTACTG GCAGGATCCCTGGCCAAAGCTCTGTGCTACATTCACAGAGTGAACAAGGCAGTTAAAGATAATCAGGAGA TGAAATCAAGGATTTTGGTGATCAAGGCTGCAGAGGACAGCGCACTGCAGTACATGAACTTCATGAACGT CATCTTTGCTGCTCAGAAGCAGAATATCCTCATCGACGCCTGCGTGCTGGACTCGGATTCAGGGCTCCTC CAGCAGGCTTGTGACATCACTGGGGGACTGTACCTGAAGGTGCCTCAGATGCCTTCTCTCCTGCAGTACT TACTGTGGGTTTTTCTTCCGGACCAAGATCAGCGGTCTCAGCTAATCCTCCCACCCCCGATCCACGTGGA CTACAGGGCTGCCTGCTTCTGTCATCGCAGTCTCATTGAGATTGGCTATGTCTGCTCTGTGTGTCTGTCT ATTTTCTGCAATTTCAGCCCCATCTGCACCACGTGCGAGACAGCTTTTAAGATCTCCCTCCCTCCTGTGC TGAAGGCCAAGAAGAAGAAACAGAAGGTGTCCCTGTGACCAGCGCCACAGGCTTCCCCAGCTCGTCCCAC AGGCTGTAGGGAGCATGGAGGAATTCTTTGAAACACTGCTCTTGCCCTGTGCTTCCAGGGTGTGAAGCTG CAGAGGACCCACACCTCCGATTTCTCTGGGAAGGGGGACAGGTGTACTTATTTGTTGTTGTAGGGTAGCA GTTGCCTGGCAGATGCTAAGCTGTTGCTGTTTTGTTGTCGCTTTGATTTTGGTGCTGGGTTTTGAAGCCC AGCCTTGCAGGAGCCAGGCCGGCAGCCCCGCACTGAGCTGCAGTCAGCTCCCTGGGACTCTCTGTTCAGA TGATCCTCTGTCAGCGCTTATGGCGGATGCTGGGTCTTCTGCACTTCAGGATTGGTAGTGTGTGAAGTTG GATGCCATTTGGGAAGATGACCAGCATTGGGTCACGGCAGCAGTCATAACAGAGCATCTTAGTTGCTCAT GGTACACTGCTAGAACCTTGAACCTGGGAGGCTGAGACAGGAAAGTTTTGGGTTCAGATCCTGCCTGGGA TGTGTAGAAGATCCTGGCTTTTTTTTTTTTTTTTAAAGAACATTTTACTTAAAATAAATTGTTTCCTTTA ACTATACTCAACTGTAACCTGAGCTAAAGGCACAAGCCTTCAGTCTCAGCACTCAGGGAGCAGAGACAGG TGTATCTCTGAGTTTGAGGCCAGCCTGGTCTACAGAGGGAGTTCAAACCAACCAGGGTTGCATCTTGCCA CCTTTTTTACAAAAATATAAAAACTAGTAACATTAACATTGGAGAACTTTTGAATACTAGACATGTAAAT GAGAAACATTTGTAATCTCAACAATAAAAATCCTATTTTTATATTTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4504077 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4504077 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002982 same experiment
	EMAGE:21485 same embryo
	EMAGE:21483 same embryo
	EMAGE:21484 same embryo
	EMAGE:21480 same embryo
	EMAGE:21482 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source